#	Pagina
attuale pagina	/open-h2020/projects/222289/index.html

Opendata, web and dolomites

H2AH2B_Propagation SIGNED

Elucidating the propagation and function of H2A and H2B modifications across DNA replication

Total Cost €

EC-Contrib. €

Partnership

Views

H2AH2B_Propagation project word cloud

Explore the words cloud of the H2AH2B_Propagation project. It provides you a very rough idea of what is the project "H2AH2B_Propagation" about.

dimers mouse transmitted division gain identity modifications unveil accurately insights replication symmetric employing ubiquitin opportunity avenues histones depletion stem cellular cell replicated maintained mutants dna propagation h2b h2a h3 inheritance mechanisms h4 crosstalk chromatin ubiquitination kinetics correct restoring symmetrically degron strands hence principles first histone propagated reveal gene marks mechanistic accompanying epigenetic pioneering me researcher expression compromised missing cells seminal strand daughter spectrometry genomic combination h2aub ligases fundamental ubiquitinated independent reestablishment mass mark dependent reestablished give h2bub follow landscape parental embryonic sequencing

Project "H2AH2B_Propagation" data sheet

The following table provides information about the project.

Coordinator	KOBENHAVNS UNIVERSITET Organization address address: NORREGADE 10 city: KOBENHAVN postcode: 1165 website: www.ku.dk contact info title: n.a. name: n.a. surname: n.a. function: n.a. email: n.a. telephone: n.a. fax: n.a.
Coordinator Country	Denmark [DK]
Total cost	207˙312 €
EC max contribution	207˙312 € (100%)
Programme	1. H2020-EU.1.3.2. (Nurturing excellence by means of cross-border and cross-sector mobility)
Code Call	H2020-MSCA-IF-2018
Funding Scheme	MSCA-IF-EF-ST
Starting year	2020
Duration (year-month-day)	from 2020-07-01 to 2022-06-30

Partnership

Take a look of project's partnership.

#	participants	country	role	EC contrib. [€]
1	KOBENHAVNS UNIVERSITET KOBENHAVNS UNIVERSITET Organization address address: NORREGADE 10 city: KOBENHAVN postcode: 1165 website: www.ku.dk contact info title: n.a. name: n.a. surname: n.a. function: n.a. email: n.a. telephone: n.a. fax: n.a.	DK (KOBENHAVN)	coordinator	207˙312.00

Map

Project objective

Chromatin and its accompanying histone marks are fundamental for correct, cell type-specific gene expression and thus important to ensure cellular identity of each cell. Importantly, this requires that the chromatin landscape is accurately propagated and maintained across DNA replication, a process which is still not well understood. While there have been insights into how histone H3 and H4 marks are transmitted to daughter cells, information on H2A and H2B marks are still missing. Using mouse embryonic stem cells, I will gain first insights into how modifications on H2A and H2B are maintained during DNA replication, and their impact on restoring the parental chromatin landscape on replicated DNA, with the following three specific objectives:

(1) I will investigate the propagation principles and kinetics of H2A/H2B modifications during DNA replication using a combination of advanced mass spectrometry and genomic approaches with a focus on ubiquitinated H2A/H2B. (2) I will gain mechanistic insights into the importance of H2A/H2B ubiquitination on chromatin reestablishment with a degron-based depletion approach targeting H2A/H2B ubiquitin ligases. This will allow me to study if, where and how the H2Aub and H2Bub-dependent histone marks are reestablished and hence reveal important features of histone mark crosstalk during DNA replication. (3) I will determine whether H2A-H2B dimers are propagated symmetrically to the two daughter strands by employing a strand-specific sequencing approach, and test mutants wherein symmetric H3-H4 propagation is compromised.

This proposal will provide pioneering insights into how histones H2A and H2B are propagated and unveil the epigenetic inheritance of their marks, and also bring seminal understanding of histone mark crosstalk during DNA replication and across cell division. This will open new research avenues in chromatin research and give me an unique opportunity to follow up on these mechanisms as future, independent researcher.

Are you the coordinator (or a participant) of this project? Plaese send me more information about the "H2AH2B_PROPAGATION" project.

For instance: the website url (it has not provided by EU-opendata yet), the logo, a more detailed description of the project (in plain text as a rtf file or a word file), some pictures (as picture files, not embedded into any word file), twitter account, linkedin page, etc.

Send me an email (fabio@fabiodisconzi.com) and I put them in your project's page as son as possible.

Thanks. And then put a link of this page into your project's website.

The information about "H2AH2B_PROPAGATION" are provided by the European Opendata Portal: CORDIS opendata.