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UTPE PEB SIGNED
Ultrahigh-throughput protein evolution for polyethylene biodegradation
Total Cost €
0EC-Contrib. €
0Partnership
0Views
0UTPE PEB project word cloud
Explore the words cloud of the UTPE PEB project. It provides you a very rough idea of what is the project "UTPE PEB" about.
water
evolution
flow
ebi
chassis
environmental
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microbial
droplet
first
differential
plastic
century
degradation
measuring
multidisciplinary
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degraded
polyethylene
strains
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microbiology
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ultrahigh
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evolutionarily
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dr
library
assay
pressing
isolate
pe
ways
polymer
scattering
university
sequencing
effort
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skills
host
standard
throughput
sme
random
assays
convey
space
abundantly
bioinformatics
nature
mutagenesis
undertaken
personal
biotechnology
inefficient
secondments
ucl
persist
hollfelder
droplets
cambridge
picoliter
modern
waste
genotypes
microfluidics
enzymes
solution
sequence
facilities
pollution
biochemistry
protein
practical
genetically
techniques
molecular
efficiency
optimise
express
screens
group
oil
extensive
associate
light
sufficiently
degrading
generation
functionally
cover
sized
synthetic
genotype
drop
cytometry
Project "UTPE PEB" data sheet
The following table provides information about the project.
| Coordinator |
THE CHANCELLOR MASTERS AND SCHOLARSOF THE UNIVERSITY OF CAMBRIDGE
Organization address contact info |
| Coordinator Country | United Kingdom [UK] |
| Total cost | 224˙933 € |
| EC max contribution | 224˙933 € (100%) |
| Programme |
1. H2020-EU.1.3.2. (Nurturing excellence by means of cross-border and cross-sector mobility) |
| Code Call | H2020-MSCA-IF-2019 |
| Funding Scheme | MSCA-IF-EF-RI |
| Starting year | 2020 |
| Duration (year-month-day) | from 2020-08-01 to 2022-07-31 |
Partnership
Take a look of project's partnership.
| # | ||||
|---|---|---|---|---|
| 1 | THE CHANCELLOR MASTERS AND SCHOLARSOF THE UNIVERSITY OF CAMBRIDGE | UK (CAMBRIDGE) | coordinator | 224˙933.00 |
Map
Project objective
The build-up of plastic pollution is one of the most pressing environmental concerns. Polyethylene (PE), the most abundantly produced plastic polymer, can persist in nature for over a century. The microbial biodegradation of PE that has been observed is slow and inefficient. So far no effort has been undertaken to improve the efficiency of enzymes involved in the biodegradation of PE through directed protein evolution. Standard assays for measuring degradation rates are not sufficiently high-throughput to cover the sequence space required. I propose to use state-of-the-art protein evolution technology to overcome this problem in two ways. First, an ultrahigh-throughput microfluidics based approach, that can associate a given genotype with its phenotype in picoliter sized water-in-oil droplets, will be used to isolate the desired genotypes from a random mutagenesis library. Second, a novel assay for measuring polymer concentration within each droplet based on differential light scattering as the polymer is degraded will assay the PE degradation rate for a given enzyme. These techniques were developed in the research group of the proposed host, Dr. Hollfelder in the Department of Biochemistry at the University of Cambridge. Using these techniques, I will functionally express and evolutionarily optimise a range of PE degrading enzymes in genetically tractable host strains, creating a chassis to investigate the potential of microbial biodegradation as a solution to plastic waste. Secondments at the EBI, UCL and the SME Drop-Tech will convey practical skills in bioinformatics screens and droplet formation. The host group’s experience in enzyme biotechnology and directed protein evolution as well as its extensive modern facilities for microfluidics, next generation sequencing and flow cytometry will synergize with my personal research experience in synthetic, molecular and microbiology to find a multidisciplinary solution to the growing problem of plastic degradation.
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The information about "UTPE PEB" are provided by the European Opendata Portal: CORDIS opendata.
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