STRESS-PROTEOSTASIS

System-wide analysis of intrinsic protein properties affecting proteome homeostasis upon abiotic stress in green algae

Coordinatore	TECHNISCHE UNIVERSITAET KAISERSLAUTERN    more  Organization address address: GOTTLIEB-DAIMLER-STRASSE Geb. 47 city: KAISERSLAUTERN postcode: 67663 contact info Titolo: Dr. Nome: Jörg Cognome: Hansen Email: send email Telefono: +49 6312055065 Fax: +49 631 205 4380
Nazionalità Coordinatore	Germany [DE]
Totale costo	100˙000 €
EC contributo	100˙000 €
Programma	FP7-PEOPLE Specific programme "People" implementing the Seventh Framework Programme of the European Community for research, technological development and demonstration activities (2007 to 2013)
Code Call	FP7-PEOPLE-2013-CIG
Funding Scheme	MC-CIG
Anno di inizio	2014
Periodo (anno-mese-giorno)	2014-03-01   -   2018-02-28

 Partecipanti

#	participant	country	role	EC contrib. [€]
1	TECHNISCHE UNIVERSITAET KAISERSLAUTERN  Organization address address: GOTTLIEB-DAIMLER-STRASSE Geb. 47 city: KAISERSLAUTERN postcode: 67663 contact info Titolo: Dr. Nome: Jörg Cognome: Hansen Email: send email Telefono: +49 6312055065 Fax: +49 631 205 4380	DE (KAISERSLAUTERN)	coordinator	100˙000.00

Mappa

 Word cloud

Esplora la "nuvola delle parole (Word Cloud) per avere un'idea di massima del progetto.

 Obiettivo del progetto (Objective)

'Abiotic environmental stresses such as extreme temperatures represent serious threats to plants and are a main reason for significant agricultural crop losses every year. Hence, an understanding of the molecular consequences of stress exposure and the underlying principles of plant stress responses are fundamental questions in biology and provide the basis for the genetic engineering of stress resistant crop plants. In particular, high temperature stress leads to an increase in protein unfolding, misfolding, and aggregation and thus challenges proteome homeostasis (proteostasis). As a response, cells increase the expression of molecular chaperones to maintain proteostasis by counteracting protein misfolding and aggregation. The overall goal of this study is to globally identify properties of fragile proteins in plant proteomes challenged by heat-shock exposure. We will use the unicellular green algae Chlamydomonas reinhardtii and Nannochloropsis sp. as model organisms to monitor protein misfolding and aggregation under physiological conditions, mild stress and severe temperature stress. We will apply quantitative proteomics and bioinformatics to reveal physicochemical properties characteristic for proteins prone to misfolding and aggregation. By this, we will further identify proteins that have a high dependence on protection by molecular chaperones. Based on this system-wide analysis individual proteins will be engineered with the goal to optimize their conformational stability for improved stress tolerance.'