QUESTFORMD

Quantitative functional assessment of gene therapeutics for Muscular Dystrophy

Coordinatore	KING'S COLLEGE LONDON    more  Organization address address: Strand city: LONDON postcode: WC2R 2LS contact info Titolo: Mr. Nome: Paul Cognome: Labbett Email: send email Telefono: +44-0-20 7848 8184 Fax: +44-0-20 7848 8184
Nazionalità Coordinatore	United Kingdom [UK]
Totale costo	232˙427 €
EC contributo	232˙427 €
Programma	FP7-PEOPLE Specific programme "People" implementing the Seventh Framework Programme of the European Community for research, technological development and demonstration activities (2007 to 2013)
Code Call	FP7-PEOPLE-2009-IEF
Funding Scheme	MC-IEF
Anno di inizio	2010
Periodo (anno-mese-giorno)	2010-03-01   -   2012-02-29

 Partecipanti

#	participant	country	role	EC contrib. [€]
1	KING'S COLLEGE LONDON  Organization address address: Strand city: LONDON postcode: WC2R 2LS contact info Titolo: Mr. Nome: Paul Cognome: Labbett Email: send email Telefono: +44-0-20 7848 8184 Fax: +44-0-20 7848 8184	UK (LONDON)	coordinator	232˙427.20

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 Obiettivo del progetto (Objective)

'Dystrophin is a large cytoskeletal protein that makes a bridge between the cytoskeletal actin microfilaments in muscle cells and the extracellular matrix, through the Dystrophin-Dystroglycan complex located at the plasma membrane. This strong link is responsible for conferring resistance to muscle contraction. Duchenne Muscular Dystrophy (DMD) is an X-chromosome linked disease caused by a mutation on the Dystrophin gene that results in damage to muscle fibers. Affected individuals rarely live beyond the third decade of life. Gene therapy is being trialed to replace the mutant protein. Since Dystrophin is a large protein, proposed gene therapies employ truncated versions of Dystrophin. Therapy faces delays because of poor understanding of the protein stability and levels required for functional recovery in the in vivo context. This proposed project aims to explore how Dystrophin stability and turnover is modulated by its structural domains, taking advantage of cutting edge in vivo imaging techniques applied to the zebrafish model. Several truncated Dystrophin constructs, containing different combinations of specific rod regions, are used on trials. The hypothesis that these differ in their ability to replace natural Dystrophin will be tested. By imaging Dystrophin-GFP fusions after genetic manipulation of single cells in the live animal, we will determine Dystrophin dynamics and function in the living organism at the single cell level, leading to insights into cytoskeletal biology. We will test the ability of truncated human Dystrophins to rescue zebrafish dystrophin mutants and determine how much, and when, Dystrophin is required to rescue muscle structure and function. Our proposal strongly promotes transfer of knowledge between developmental biology and applied clinical research. The project will establish the applicant as a key member of a multidisciplinary international team aiming to develop time- and cost-effective initial screens for gene therapeutics.'