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CRASCI SIGNED

Spatial-temporal characteristics of Cortical Reorganization after Spinal Cord Injury and the role of interneurons and astrocytes

Total Cost €

EC-Contrib. €

Partnership

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CRASCI project word cloud

Explore the words cloud of the CRASCI project. It provides you a very rough idea of what is the project "CRASCI" about.

signaling layer area reorganization time points inputs sci mice followed gfp calcium sensory local therapies gq indicators deprived plasticity cord transmission intact activated dreadds heterogeneous neuropathic model neuronal depending multielectrode suggest gcamp6 cell monitoring vitro astrocytes first array somatosensory interneurons vivo spatial stimulation signals determined s1 regions temporal cortex recording forelimb data indicator inhibitory elapsed manipulating functional spinal dreadd hindlimb encoded transgenic expressing layers patterns electrophysiology primary surrounding types intracellular decrease involvement astrocyte gabaergic brain vertical injury cells pathologies cortical complexity thoracic pain ip2r2 genetically either network chemogenetics ca2

Project "CRASCI" data sheet

The following table provides information about the project.

Coordinator	FUNDACION HOSPITAL NACIONAL DE PARAPLEJICOS Organization address address: CARRETERA DE LA PERALEDA S/N city: TOLEDO postcode: 45071 website: www.infomedula.org contact info title: n.a. name: n.a. surname: n.a. function: n.a. email: n.a. telephone: n.a. fax: n.a.
Coordinator Country	Spain [ES]
Total cost	170˙121 €
EC max contribution	170˙121 € (100%)
Programme	1. H2020-EU.1.3.2. (Nurturing excellence by means of cross-border and cross-sector mobility)
Code Call	H2020-MSCA-IF-2017
Funding Scheme	MSCA-IF-EF-SE
Starting year	2018
Duration (year-month-day)	from 2018-08-01 to 2020-07-31

Partnership

Take a look of project's partnership.

#	participants	country	role	EC contrib. [€]
1	FUNDACION HOSPITAL NACIONAL DE PARAPLEJICOS FUNDACION HOSPITAL NACIONAL DE PARAPLEJICOS Organization address address: CARRETERA DE LA PERALEDA S/N city: TOLEDO postcode: 45071 website: www.infomedula.org contact info title: n.a. name: n.a. surname: n.a. function: n.a. email: n.a. telephone: n.a. fax: n.a.	ES (TOLEDO)	coordinator	170˙121.00

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Project objective

Spinal cord injury (SCI) is followed by functional reorganization of the primary somatosensory cortex (S1), in which the S1 area deprived of inputs is activated by sensory stimulation of surrounding intact regions. Recent data suggest that reorganization after SCI is a heterogeneous process depending on the time elapsed after injury and the cortical layer under study. This research proposal aims to study the complexity of the cortical reorganization after SCI in terms of spatial-temporal patterns and the involvement of distinct cell types as inhibitory interneurons and astrocytes. This will be achieved by monitoring and manipulating brain activity using in vivo and in vitro electrophysiology, genetically encoded calcium indicators (GCaMP6), chemogenetics (DREADDs) and transgenic mice. A mice model of thoracic SCI will be used throughout the study. First, reorganization of the hindlimb and forelimb S1 cortex at different time points after the injury will be studied by recording in vivo neuronal activity in response to sensory stimulation across all layers of S1 using a vertical multielectrode array. Second, changes in inhibitory transmission induced by SCI will be studied by monitoring intracellular Ca2 signaling and by in vitro electrophysiology from GFP expressing GABAergic cells. Third, the role of astrocytes in the reorganization after SCI will be studied by using either Gq DREADD to enhance astrocyte activity or IP2R2-/- mice to decrease astrocyte activity while recording in vivo neuronal responses across all layers of S1. Changes in astrocyte activity after SCI will also be determined by monitoring intracellular Ca2 signals from astrocytes expressing the calcium indicator GCaMP6. Results from this proposal will be a first in understanding the complex network of local plasticity in S1 both in control conditions and after SCI. It will be also relevant to design new therapies for SCI-associated pathologies as neuropathic pain.

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The information about "CRASCI" are provided by the European Opendata Portal: CORDIS opendata.