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RARE MAPS SIGNED
Dynamic proteomic maps of stem cell-derived neurons as a mechanistic discovery pipeline for rare neurological disease
Total Cost €
0EC-Contrib. €
0Partnership
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0RARE MAPS project word cloud
Explore the words cloud of the RARE MAPS project. It provides you a very rough idea of what is the project "RARE MAPS" about.
comparatively
cell
organellar
neurons
difficult
proteomics
neurodegenerative
cells
trafficking
workflow
differentiated
intermediate
disorder
tissue
spatial
autophagy
abundance
axon
utility
neurological
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crispr
patient
reveal
health
unbiased
borner
proteomic
knockout
mislocalisation
mature
dynamic
insights
stem
brain
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molecular
deficient
treat
discovery
lose
unmet
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combining
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mechanistic
localisation
medical
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definition
quantitative
syndrome
human
proteins
mechanisms
pluripotent
deficiency
model
axonal
implicated
pipeline
vivo
dr
mouse
detection
protein
rare
neuronal
hipscs
pathogenesis
inaccessibility
provides
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defects
screening
disorders
detect
ap
perturbation
made
cas9
phenotypic
nervous
global
Project "RARE MAPS" data sheet
The following table provides information about the project.
| Coordinator |
MAX-PLANCK-GESELLSCHAFT ZUR FORDERUNG DER WISSENSCHAFTEN EV
Organization address contact info |
| Coordinator Country | Germany [DE] |
| Total cost | 162˙806 € |
| EC max contribution | 162˙806 € (100%) |
| Programme |
1. H2020-EU.1.3.2. (Nurturing excellence by means of cross-border and cross-sector mobility) |
| Code Call | H2020-MSCA-IF-2019 |
| Funding Scheme | MSCA-IF-EF-ST |
| Starting year | 2020 |
| Duration (year-month-day) | from 2020-06-01 to 2022-05-31 |
Partnership
Take a look of project's partnership.
| # | ||||
|---|---|---|---|---|
| 1 | MAX-PLANCK-GESELLSCHAFT ZUR FORDERUNG DER WISSENSCHAFTEN EV | DE (MUENCHEN) | coordinator | 162˙806.00 |
Map
Project objective
Rare diseases are a major unmet medical need, as is the definition of the relevant disease mechanisms. Many rare diseases affect the nervous system. These are challenging to treat, and mechanistic studies are difficult due to the inaccessibility of patient tissue. Global proteomic studies have provided insight into whole tissue or cell changes in protein abundance but lose information on protein subcellular localisation, which is important because defects in protein trafficking are implicated in many neurological disorders. In ‘RARE MAPS’ I propose an unbiased mechanistic discovery pipeline combining human induced pluripotent stem cells (hiPSCs) with advanced spatial proteomics. I will use a method developed by Dr. Borner called ‘dynamic organellar maps’, which provides quantitative protein subcellular localisation information at the whole proteome level. Used comparatively, it can detect changes in protein localisation due to a perturbation, allowing unbiased screening for phenotypic changes. To develop this workflow, I will apply it to the rare neurodegenerative disorder AP-4 deficiency syndrome. AP-4 knockout hiPSCs will be differentiated into cortical neurons and maps will be made of intermediate cortical stem cells and mature cortical neurons. Comparison to control cells will enable the detection of changes to protein localisation and abundance. I will also apply the maps to brain tissue from an AP-4 deficient mouse model to detect protein mislocalisation in vivo. I will then use CRISPR/Cas9 technology to investigate the role of novel and known AP-4-associated proteins in neuronal autophagy and axonal health. This project will demonstrate the utility of dynamic organellar maps to reveal molecular mechanisms of rare neurological disorders as well as provide new insights into the pathogenesis of AP-4 deficiency and the role of protein trafficking and autophagy in the axon.
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The information about "RARE MAPS" are provided by the European Opendata Portal: CORDIS opendata.
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