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Wall-E SIGNED
Make it, don’t break it: a reconstitution screen for Casparian strip formation in the root endodermis
Total Cost €
0EC-Contrib. €
0Partnership
0Views
0Wall-E project word cloud
Explore the words cloud of the Wall-E project. It provides you a very rough idea of what is the project "Wall-E" about.
screen
root
faced
reverse
slowly
assessing
dismutases
last
selective
dirigent
lethality
functions
attempt
wall
developmental
standstill
genes
players
model
genetic
wp3
pioneer
organisms
endodermal
limitations
mechanisms
firstly
oxide
genetically
differentiation
core
super
function
laccases
crispr
elucidate
barrier
impregnations
arabidopsis
wp2
cellular
dependent
uncover
lignin
enzymes
cs
reconstitute
sufficient
molecular
thirdly
endodermis
casparian
gene
minimally
strips
expressing
peroxidases
mutant
proteins
decade
screening
polymerising
nutrients
cells
master
generation
pioneering
regulator
activation
differentiating
pleiotropy
cell
myb36
coming
transmembrane
assembly
secondly
wp1
activator
stability
combinatorial
water
introduce
causing
machinery
efficiency
activating
formed
plants
gain
redundancy
Project "Wall-E" data sheet
The following table provides information about the project.
| Coordinator |
UNIVERSITE DE LAUSANNE
Organization address contact info |
| Coordinator Country | Switzerland [CH] |
| Total cost | 191˙149 € |
| EC max contribution | 191˙149 € (100%) |
| Programme |
1. H2020-EU.1.3.2. (Nurturing excellence by means of cross-border and cross-sector mobility) |
| Code Call | H2020-MSCA-IF-2019 |
| Funding Scheme | MSCA-IF-EF-ST |
| Starting year | 2021 |
| Duration (year-month-day) | from 2021-08-01 to 2023-07-31 |
Partnership
Take a look of project's partnership.
| # | ||||
|---|---|---|---|---|
| 1 | UNIVERSITE DE LAUSANNE | CH (LAUSANNE) | coordinator | 191˙149.00 |
Map
Project objective
In plants, the root endodermis functions as a barrier, allowing the selective uptake of nutrients and water. The barrier is formed by cell wall impregnations called Casparian strips (CS), produced by differentiating endodermal cells. In the last decade, pioneering work on the endodermis has identified numerous players involved in CS formation, including transmembrane proteins, peroxidases, dirigent-like proteins, lignin polymerising enzymes, laccases and super-oxide dismutases. However, the forward and reverse genetic approaches used to uncover these proteins are slowly coming to a standstill due to their limitations when faced with gene redundancy or genes with a broader range of activities, causing pleiotropy or lethality. I now propose to use CS formation as a model in order to pioneer a combinatorial, gain-of-function screen, aiming to define a minimally sufficient gene set for the assembly of a CS. I will attempt to reconstitute a CS in the endodermis of the myb36 mutant – a master regulator of endodermal differentiation - by activation screening for genes within the MYB36-dependent gene set. Firstly, I will introduce a “core machinery” for CS formation by expressing important known players and assessing CS formation and stability (WP1). Secondly, I will test the most recently developed second generation CRISPR activator systems for their efficiency in activating genes of interest in the endodermis (WP2). Thirdly, using the CRISPR activator technology, I will screen for novel genes that will improve the formation, stability and functionality of the CS (WP3). Identifying new genes involved in CS formation through a combinatorial, gain-of-function approach represents a novel way to genetically elucidate molecular mechanisms and could become a model for other cellular and developmental processes in Arabidopsis or other organisms.
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No valid XML
lastchecktime (2024-05-12 11:06:18) correctly updated