NICHOID SIGNED
Mechanobiology of nuclear import of transcription factors modeled within a bioengineered stem cell niche.
Total Cost €
0EC-Contrib. €
0Partnership
0Views
0NICHOID project word cloud
Explore the words cloud of the NICHOID project. It provides you a very rough idea of what is the project "NICHOID" about.
Project "NICHOID" data sheet
The following table provides information about the project.
| Coordinator |
POLITECNICO DI MILANO
Organization address contact info |
| Coordinator Country | Italy [IT] |
| Project website | http://www.nichoid.polimi.it |
| Total cost | 1˙903˙330 € |
| EC max contribution | 1˙903˙330 € (100%) |
| Programme |
1. H2020-EU.1.1. (EXCELLENT SCIENCE - European Research Council (ERC)) |
| Code Call | ERC-2014-CoG |
| Funding Scheme | ERC-COG |
| Starting year | 2015 |
| Duration (year-month-day) | from 2015-05-01 to 2020-04-30 |
Partnership
Take a look of project's partnership.
| # | ||||
|---|---|---|---|---|
| 1 | POLITECNICO DI MILANO | IT (MILANO) | coordinator | 1˙802˙830.00 |
| 2 | ISTITUTO DI RICERCHE FARMACOLOGICHE MARIO NEGRI | IT (MILANO) | participant | 100˙500.00 |
Map
Project objective
Many therapeutic applications of stem cells require accurate control of their differentiation. To this purpose there is a major ongoing effort in the development of advanced culture substrates to be used as “synthetic niches” for the cells, mimicking the native ones. The goal of this project is to use a synthetic niche cell culture model to test my revolutionary hypothesis that in stem cell differentiation, nuclear import of gene-regulating transcription factors is controlled by the stretch of the nuclear pore complexes. If verified, this idea could lead to a breakthrough in biomimetic approaches to engineering stem cell differentiation. I investigate this question specifically in mesenchymal stem cells (MSC), because they are adherent and highly mechano-sensitive to architectural cues of the microenvironment. To verify my hypothesis I will use a combined experimental-computational model of mechanotransduction. I will a) scale-up an existing three-dimensional synthetic niche culture substrate, fabricated by two-photon laser polymerization, b) characterize the effect of tridimensionality on the differentiation fate of MSC cultured in the niches, c) develop a multiphysics/multiscale computational model of nuclear import of transcription factors within differentially-spread cultured cells, and d) integrate the numerical predictions with experimentally-measured import of fluorescently-labelled transcription factors. This project requires the synergic combination of several advanced bioengineering technologies, including micro/nano fabrication and biomimetics. The use of two-photon laser polymerization for controlling the geometry of the synthetic cell niches is very innovative and will highly impact the fields of bioengineering and biomaterial technology. A successful outcome will lead to a deeper understanding of bioengineering methods to direct stem cell fate and have therefore a significant impact in tissue repair technologies and regenerative medicine.
Publications
| year | authors and title | journal | last update |
|---|---|---|---|
| 2018 |
Alberto GarcÃa-González, Emanuela Jacchetti, Roberto Marotta, Marta Tunesi, José F. RodrÃguez Matas, Manuela T. Raimondi The Effect of Cell Morphology on the Permeability of the Nuclear Envelope to Diffusive Factors published pages: , ISSN: 1664-042X, DOI: 10.3389/fphys.2018.00925 |
Frontiers in Physiology 9 | 2020-04-23 |
| 2019 |
Lucia Boeri, Diego Albani, Manuela Teresa Raimondi, Emanuela Jacchetti Mechanical regulation of nucleocytoplasmic translocation in mesenchymal stem cells: characterization and methods for investigation published pages: 817-831, ISSN: 1867-2450, DOI: 10.1007/s12551-019-00594-3 |
Biophysical Reviews 11/5 | 2020-04-23 |
| 2019 |
F. Donnaloja, E. Jacchetti, M. Soncini, M. T. Raimondi Mechanosensing at the Nuclear Envelope by Nuclear Pore Complex Stretch Activation and Its Effect in Physiology and Pathology published pages: , ISSN: 1664-042X, DOI: 10.3389/fphys.2019.00896 |
Frontiers in Physiology 10 | 2020-04-23 |
| 2016 |
Garcia Gonzales, A.; Marotta, R.; Tunesi, M.; Nava, M. M.; Fedele, R.; Jacchetti, E.; Rodriguez Matas, J. F.; Raimondi, M. T. Integrated experimental/computational approach of the nuclear pore complex published pages: , ISSN: 1473-2262, DOI: |
European Cells and Materials 1 | 2020-04-23 |
| 2016 |
Michele M. Nava, Nunzia Di Maggio, Tommaso Zandrini, Giulio Cerullo, Roberto Osellame, Ivan Martin, Manuela T. Raimondi Synthetic niche substrates engineered via two-photon laser polymerization for the expansion of human mesenchymal stromal cells published pages: , ISSN: 1932-6254, DOI: 10.1002/term.2187 |
Journal of Tissue Engineering and Regenerative Medicine | 2020-04-23 |
| 2018 |
Alessandro Marturano-Kruik, Michele Maria Nava, Keith Yeager, Alan Chramiec, Luke Hao, Samuel Robinson, Edward Guo, Manuela Teresa Raimondi, Gordana Vunjak-Novakovic Human bone perivascular niche-on-a-chip for studying metastatic colonization published pages: 1256-1261, ISSN: 0027-8424, DOI: 10.1073/pnas.1714282115 |
Proceedings of the National Academy of Sciences 115/6 | 2020-04-23 |
| 2016 |
Marta Tunesi, Federica Fusco, Fabio Fiordaliso, Alessandro Corbelli, Gloria Biella, Manuela T. Raimondi Optimization of a 3D Dynamic Culturing System for In Vitro Modeling of Frontotemporal Neurodegeneration-Relevant Pathologic Features published pages: , ISSN: 1663-4365, DOI: 10.3389/fnagi.2016.00146 |
Frontiers in Aging Neuroscience 8 | 2020-04-23 |
| 2017 |
Riccardo Sacco, Paola Causin, Chiara Lelli, Manuela T. Raimondi A poroelastic mixture model of mechanobiological processes in biomass growth: theory and application to tissue engineering published pages: 3273-3297, ISSN: 0025-6455, DOI: 10.1007/s11012-017-0638-9 |
Meccanica 52/14 | 2020-04-23 |
| 2017 |
Hadis Eghbali, Michele M. Nava, Gabriella Leonardi, Davod Mohebbi-Kalhori, Roberto Sebastiano, Abdolreza Samimi, Manuela T. Raimondi An experimental-numerical investigation on the effects of macroporous scaffold geometry on cell culture parameters published pages: 185-195, ISSN: 0391-3988, DOI: 10.5301/ijao.5000554 |
The International Journal of Artificial Organs 40/4 | 2020-04-23 |
| 2016 |
Hadis Eghbali, Michele M. Nava, Davod Mohebbi-Kalhori, Manuela T. Raimondi Hollow fiber bioreactor technology for tissue engineering applications published pages: 1-15, ISSN: 0391-3988, DOI: 10.5301/ijao.5000466 |
The International Journal of Artificial Organs 39/1 | 2020-04-23 |
| 2016 |
Michele M. Nava, Roberto Fedele, Manuela T. Raimondi Computational prediction of strain-dependent diffusion of transcription factors through the cell nucleus published pages: 983-993, ISSN: 1617-7959, DOI: 10.1007/s10237-015-0737-2 |
Biomechanics and Modeling in Mechanobiology 15/4 | 2020-04-23 |
| 2016 |
Laura Iannetti, Giovanna D’Urso, Gioacchino Conoscenti, Elena Cutrì, Rocky S. Tuan, Manuela T. Raimondi, Riccardo Gottardi, Paolo Zunino Distributed and Lumped Parameter Models for the Characterization of High Throughput Bioreactors published pages: e0162774, ISSN: 1932-6203, DOI: 10.1371/journal.pone.0162774 |
PLOS ONE 11/9 | 2020-04-23 |
| 2016 |
Alberto Garcia, Jose F. Rodriguez Matas, Manuela T. Raimondi Modeling of the mechano-chemical behaviour of the nuclear pore complex: current research and perspectives published pages: 1011-1021, ISSN: 1757-9694, DOI: 10.1039/C6IB00153J |
Integr. Biol. 8/10 | 2020-04-23 |
| 2015 |
Manuela T. Raimondi, Carmen Giordano, Riccardo Pietrabissa Oxygen measurement in interstitially perfused cellularized constructs cultured in a miniaturized bioreactor published pages: 0-0, ISSN: 2280-8000, DOI: 10.5301/jabfm.5000246 |
Journal of Applied Biomaterials & Functional Materials 13/4 | 2020-04-23 |
| 2016 |
Michele M. Nava, Alessio Piuma, Marina Figliuzzi, Irene Cattaneo, Barbara Bonandrini, Tommaso Zandrini, Giulio Cerullo, Roberto Osellame, Andrea Remuzzi, Manuela T. Raimondi Two-photon polymerized “nichoid†substrates maintain function of pluripotent stem cells when expanded under feeder-free conditions published pages: , ISSN: 1757-6512, DOI: 10.1186/s13287-016-0387-z |
Stem Cell Research & Therapy 7/1 | 2020-04-23 |
| 2017 |
Davide Ricci, Michele Nava, Tommaso Zandrini, Giulio Cerullo, Manuela Raimondi, Roberto Osellame Scaling-Up Techniques for the Nanofabrication of Cell Culture Substrates via Two-Photon Polymerization for Industrial-Scale Expansion of Stem Cells published pages: 66, ISSN: 1996-1944, DOI: 10.3390/ma10010066 |
Materials 10/1 | 2020-04-23 |
Are you the coordinator (or a participant) of this project? Plaese send me more information about the "NICHOID" project.
For instance: the website url (it has not provided by EU-opendata yet), the logo, a more detailed description of the project (in plain text as a rtf file or a word file), some pictures (as picture files, not embedded into any word file), twitter account, linkedin page, etc.
Send me an email (fabio@fabiodisconzi.com) and I put them in your project's page as son as possible.
Thanks. And then put a link of this page into your project's website.
The information about "NICHOID" are provided by the European Opendata Portal: CORDIS opendata.