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BIZEB SIGNED

Bio-Imaging of Zoonotic and Emerging Bunyaviruses

Total Cost €

EC-Contrib. €

Partnership

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Outcomes and
results

BIZEB project word cloud

Explore the words cloud of the BIZEB project. It provides you a very rough idea of what is the project "BIZEB" about.

chosen applicable virus vaccine form vaccines benefits membrane animals rational microscopy maximise fluorescence antivirals broadly resolution medically bio classes receptor cell approved bunyaviruses biology probe understand cutting spatial computational pose harbour structures complexes mechanism dynamics edge pave notably dengue protein drug proteins significance temporal viral laboratory human electron fuses world host 3 enveloped imaging determined entry techniques biological mediated molecular class biosafety viruses post structural exist threat solved conformation aring cellular deciphering infectious fusion vitro vivo tomography flexible cryo model questions unanswered image humans similarly interesting

Project "BIZEB" data sheet

The following table provides information about the project.

Coordinator	HELSINGIN YLIOPISTO Organization address address: YLIOPISTONKATU 3 city: HELSINGIN YLIOPISTO postcode: 14 website: www.helsinki.fi contact info title: n.a. name: n.a. surname: n.a. function: n.a. email: n.a. telephone: n.a. fax: n.a.
Coordinator Country	Finland [FI]
Project website	https://www.strubi.ox.ac.uk/research/juha-huiskonen
Total cost	1˙998˙375 €
EC max contribution	1˙998˙375 € (100%)
Programme	1. H2020-EU.1.1. (EXCELLENT SCIENCE - European Research Council (ERC))
Code Call	ERC-2014-CoG
Funding Scheme	ERC-COG
Starting year	2015
Duration (year-month-day)	from 2015-04-01 to 2020-03-31

Partnership

Take a look of project's partnership.

#	participants	country	role	EC contrib. [€]
1	HELSINGIN YLIOPISTO HELSINGIN YLIOPISTO Organization address address: YLIOPISTONKATU 3 city: HELSINGIN YLIOPISTO postcode: 14 website: www.helsinki.fi contact info title: n.a. name: n.a. surname: n.a. function: n.a. email: n.a. telephone: n.a. fax: n.a.	FI (HELSINGIN YLIOPISTO)	coordinator	652˙741.00
2	THE CHANCELLOR, MASTERS AND SCHOLARS OF THE UNIVERSITY OF OXFORD THE CHANCELLOR, MASTERS AND SCHOLARS OF THE UNIVERSITY OF OXFORD Organization address address: WELLINGTON SQUARE UNIVERSITY OFFICES city: OXFORD postcode: OX1 2JD website: www.ox.ac.uk contact info title: n.a. name: n.a. surname: n.a. function: n.a. email: n.a. telephone: n.a. fax: n.a.	UK (OXFORD)	participant	1˙345˙633.00

Map

Project objective

We aim to understand host cell entry of enveloped viruses at molecular level. A crucial step in this process is when the viral membrane fuses with the cell membrane. Similarly to cell–cell fusion, this step is mediated by fusion proteins (classes I–III). Several medically important viruses, notably dengue and many bunyaviruses, harbour a class II fusion protein. Class II fusion protein structures have been solved in pre- and post-fusion conformation and in some cases different factors promoting fusion have been determined. However, questions about the most important steps of this key process remain unanswered. I will focus on the entry mechanism of bunyaviruses by using cutting-edge, high spatial and temporal resolution bio-imaging techniques. These viruses have been chosen as a model system to maximise the significance of the project: they form an emerging viral threat to humans and animals, no approved vaccines or antivirals exist for human use and they are less studied than other class II fusion protein systems. Cryo-electron microscopy and tomography will be used to solve high-resolution structures (up to ~3 Å) of viruses, in addition to virus–receptor and virus–membrane complexes. Advanced fluorescence microscopy techniques will be used to probe the dynamics of virus entry and fusion in vivo and in vitro. Deciphering key steps in virus entry is expected to contribute to rational vaccine and drug design. During this project I aim to establish a world-class laboratory in structural and cellular biology of emerging viruses. The project greatly benefits from our unique biosafety level 3 laboratory offering advanced bio-imaging techniques. Furthermore it will also pave way for similar projects on other infectious viruses. Finally the novel computational image processing methods developed in this project will be broadly applicable for the analysis of flexible biological structures, which often pose the most challenging yet interesting questions in structural biology.

Publications

List of publications.
year	authors and title	journal	last update
2019	Kamel El Omari, Sai Li, Abhay Kotecha, Thomas S. Walter, Eduardo A. Bignon, Karl Harlos, Pentti Somerharju, Felix De Haas, Daniel K. Clare, Mika Molin, Felipe Hurtado, Mengqiu Li, Jonathan M. Grimes, Dennis H. Bamford, Nicole D. Tischler, Juha T. Huiskonen, David I. Stuart, Elina Roine The structure of a prokaryotic viral envelope protein expands the landscape of membrane fusion proteins published pages: , ISSN: 2041-1723, DOI: 10.1038/s41467-019-08728-7	Nature Communications 10/1	2019-09-02
2018	Max Renner, Aleksandra Flanagan, Wanwisa Dejnirattisai, Chunya Puttikhunt, Watchara Kasinrerk, Piyada Supasa, Wiyada Wongwiwat, Kriangkrai Chawansuntati, Thaneeya Duangchinda, Alison Cowper, Claire M. Midgley, Prida Malasit, Juha T. Huiskonen, Juthathip Mongkolsapaya, Gavin R. Screaton, Jonathan M. Grimes Characterization of a potent and highly unusual minimally enhancing antibody directed against dengue virus published pages: 1248-1256, ISSN: 1529-2908, DOI: 10.1038/s41590-018-0227-7	Nature Immunology 19/11	2019-09-02
2018	Yasunori Watanabe, Jayna Raghwani, Joel D. Allen, Gemma E. Seabright, Sai Li, Felipe Moser, Juha T. Huiskonen, Thomas Strecker, Thomas A. Bowden, Max Crispin Structure of the Lassa virus glycan shield provides a model for immunological resistance published pages: 7320-7325, ISSN: 0027-8424, DOI: 10.1073/pnas.1803990115	Proceedings of the National Academy of Sciences 115/28	2019-09-02
2019	Luigi De Colibus, Elina Roine, Thomas S. Walter, Serban L. Ilca, Xiangxi Wang, Nan Wang, Alan M. Roseman, Dennis Bamford, Juha T. Huiskonen, David I. Stuart Assembly of complex viruses exemplified by a halophilic euryarchaeal virus published pages: , ISSN: 2041-1723, DOI: 10.1038/s41467-019-09451-z	Nature Communications 10/1	2019-09-02
2019	Serban L. Ilca, Xiaoyu Sun, Kamel El Omari, Abhay Kotecha, Felix de Haas, Frank DiMaio, Jonathan M. Grimes, David I. Stuart, Minna M. Poranen, Juha T. Huiskonen Multiple liquid crystalline geometries of highly compacted nucleic acid in a dsRNA virus published pages: 252-256, ISSN: 0028-0836, DOI: 10.1038/s41586-019-1229-9	Nature 570/7760	2019-09-02
2019	CÃ©sar LÃ³pez-Camacho, Young Chan Kim, Joshua Blight, Marcos Lazaro Moreli, Eduardo Montoya-Diaz, Juha T Huiskonen, Beate Mareike KÃ¼mmerer, Arturo Reyes-Sandoval Assessment of Immunogenicity and Neutralisation Efficacy of Viral-Vectored Vaccines Against Chikungunya Virus published pages: 322, ISSN: 1999-4915, DOI: 10.3390/v11040322	Viruses 11/4	2019-09-02
2018	Elizabeth R. Allen, Stefanie A. Krumm, Jayna Raghwani, Steinar Halldorsson, Angela Elliott, Victoria A. Graham, Elina Koudriakova, Karl Harlos, Daniel Wright, George M. Warimwe, Benjamin Brennan, Juha T. Huiskonen, Stuart D. Dowall, Richard M. Elliott, Oliver G. Pybus, Dennis R. Burton, Roger Hewson, Katie J. Doores, Thomas A. Bowden A Protective Monoclonal Antibody Targets a Site of Vulnerability on the Surface of Rift Valley Fever Virus published pages: 3750-3758.e4, ISSN: 2211-1247, DOI: 10.1016/j.celrep.2018.12.001	Cell Reports 25/13	2019-09-02
2015	Serban L. Ilca, Abhay Kotecha, Xiaoyu Sun, Minna M. Poranen, David I. Stuart, Juha T. Huiskonen Localized reconstruction of subunits from electron cryomicroscopy images of macromolecular complexes published pages: 8843, ISSN: 2041-1723, DOI: 10.1038/ncomms9843	Nature Communications 6	2019-09-02
2018	Steinar Halldorsson, Sai Li, Mengqiu Li, Karl Harlos, Thomas A. Bowden, Juha T. Huiskonen Shielding and activation of a viral membrane fusion protein published pages: , ISSN: 2041-1723, DOI: 10.1038/s41467-017-02789-2	Nature Communications 9/1	2019-09-02
2017	Nayab Malik, Abhay Kotecha, Sarah Gold, Amin Asfor, Jingshan Ren, Juha T. Huiskonen, Tobias J. Tuthill, Elizabeth E. Fry, David I. Stuart Structures of foot and mouth disease virus pentamers: Insight into capsid dissociation and unexpected pentamer reassociation published pages: e1006607, ISSN: 1553-7374, DOI: 10.1371/journal.ppat.1006607	PLOS Pathogens 13/9	2019-09-02
2017	Ilona Rissanen, Robert Stass, Antra Zeltina, Sai Li, Jussi Hepojoki, Karl Harlos, Robert J. C. Gilbert, Juha T. Huiskonen, Thomas A. Bowden Structural Transitions of the Conserved and Metastable Hantaviral Glycoprotein Envelope published pages: e00378-17, ISSN: 0022-538X, DOI: 10.1128/JVI.00378-17	Journal of Virology 91/21	2019-09-02
2017	Mariana Grieben, Ashley C W Pike, Chitra A Shintre, Elisa Venturi, Sam El-Ajouz, Annamaria Tessitore, Leela Shrestha, Shubhashish Mukhopadhyay, Pravin Mahajan, Rod Chalk, Nicola A Burgess-Brown, Rebecca Sitsapesan, Juha T Huiskonen, Elisabeth P Carpenter Structure of the polycystic kidney disease TRP channel Polycystin-2 (PC2) published pages: 114-122, ISSN: 1545-9993, DOI: 10.1038/nsmb.3343	Nature Structural & Molecular Biology 24/2	2019-09-02
2017	Zhaoyang Sun, Kamel El Omari, Xiaoyu Sun, Serban L. Ilca, Abhay Kotecha, David I. Stuart, Minna M. Poranen, Juha T. Huiskonen Double-stranded RNA virus outer shell assembly by bona fide domain-swapping published pages: 14814, ISSN: 2041-1723, DOI: 10.1038/ncomms14814	Nature Communications 8	2019-09-02
2016	Renner, Max; Leyrat, CÃ©dric; Huiskonen, Juha T; Paesen, Guido C; Bertinelli, Mattia; Saraiva de Oliveira, Laura Freitas; Grimes, Jonathan M. Nucleocapsid assembly in pneumoviruses is regulated by conformational switching of the N protein published pages: , ISSN: 2050-084X, DOI: 10.7554/eLife.12627	eLife	2019-09-02
2016	Sai Li; Ilona Rissanen; Jayna Raghwani; ThomasÂ A. Bowden; JuhaÂ T. Huiskonen; Antra Zeltina; Jussi Hepojoki; Karl Harlos; OliverÂ G. Pybus A Molecular-Level Account of the Antigenic Hantaviral Surface published pages: , ISSN: 2211-1247, DOI: 10.1016/j.celrep.2016.03.082	Cell Reports 1	2019-09-02
2017	Denis Ptchelkine, Ashley Gillum, Tomohiro Mochizuki, Soizick Lucas-Staat, Ying Liu, Mart Krupovic, Simon E. V. Phillips, David Prangishvili, Juha T. Huiskonen Unique architecture of thermophilic archaeal virus APBV1 and its genome packaging published pages: , ISSN: 2041-1723, DOI: 10.1038/s41467-017-01668-0	Nature Communications 8/1	2019-09-02
2017	Abhay Kotecha, Quan Wang, Xianchi Dong, Serban L. Ilca, Marina Ondiviela, Rao Zihe, Julian Seago, Bryan Charleston, Elizabeth E. Fry, Nicola G. A. Abrescia, Timothy A. Springer, Juha T. Huiskonen, David I. Stuart Rules of engagement between Î±vÎ²6 integrin and foot-and-mouth disease virus published pages: 15408, ISSN: 2041-1723, DOI: 10.1038/ncomms15408	Nature Communications 8	2019-09-02
2016	Bitto, David; Halldorsson, Steinar; Huiskonen, Juha T; Caputo, Alessandro Low pH and Anionic Lipid-dependent Fusion of Uukuniemi Phlebovirus to Liposomes* published pages: , ISSN: 1083-351X, DOI: 10.1074/jbc.M115.691113	The Journal of Biological Chemistry	2019-09-02
2016	Steinar Halldorsson, Anna-Janina Behrens, Karl Harlos, Juha T. Huiskonen, Richard M Elliott, Max Crispin, Benjamin Brennan, and Thomas A Bowden Structure of a phleboviral envelope glycoprotein reveals a consolidated model of membrane fusion published pages: , ISSN: 1091-6490, DOI: 10.1073/pnas.1603827113	P Natl Acad Sci Usa	2019-09-02
2017	Martin Wilkes, M Gregor Madej, Lydia Kreuter, Daniel Rhinow, Veronika Heinz, Silvia De Sanctis, Sabine Ruppel, Rebecca M Richter, Friederike Joos, Marina Grieben, Ashley C W Pike, Juha T Huiskonen, Elisabeth P Carpenter, Werner KÃ¼hlbrandt, Ralph Witzgall, Christine Ziegler Molecular insights into lipid-assisted Ca2+ regulation of the TRP channel Polycystin-2 published pages: 123-130, ISSN: 1545-9993, DOI: 10.1038/nsmb.3357	Nature Structural & Molecular Biology 24/2	2019-09-02

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