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minimal-phagocyte SIGNED

Reconstitution of the basic molecular mechanism of phagocytosis – a bottom-up synthetic biology approach

Total Cost €

EC-Contrib. €

Partnership

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Outcomes and
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minimal-phagocyte project word cloud

Explore the words cloud of the minimal-phagocyte project. It provides you a very rough idea of what is the project "minimal-phagocyte" about.

cutting ultimately polymerisation cellular cortex 2020 deforms objects gap engulfment receptors creation little microorganisms molecular vesicles always innovation protocells innate horizon edge line giant mobility functions artificial drivers nevertheless answer closure thereby cells reconstitute tissue guvs biotechnologies sufficient cues affinity engulf reorganisation wrap biology signalling players requiring immunity individual era biophysics particle internalised reshapes dynamic synthetic questions wrapping phagocyte invading cell ant biophysical unilamellar supporting ing basis minimal abscission basic cup net spatial render cytoskeleton drug phagocytic initiation components binding process phagocytosis directed actin biotechnological apoptotic membrane model fill remodelling erasynbio

Project "minimal-phagocyte" data sheet

The following table provides information about the project.

Coordinator	MAX-PLANCK-GESELLSCHAFT ZUR FORDERUNG DER WISSENSCHAFTEN EV Organization address address: HOFGARTENSTRASSE 8 city: Munich postcode: 80539 website: www.mpg.de contact info title: n.a. name: n.a. surname: n.a. function: n.a. email: n.a. telephone: n.a. fax: n.a.
Coordinator Country	Germany [DE]
Total cost	159˙460 €
EC max contribution	159˙460 € (100%)
Programme	1. H2020-EU.1.3.2. (Nurturing excellence by means of cross-border and cross-sector mobility)
Code Call	H2020-MSCA-IF-2015
Funding Scheme	MSCA-IF-EF-ST
Starting year	2016
Duration (year-month-day)	from 2016-09-01 to 2018-08-31

Partnership

Take a look of project's partnership.

#	participants	country	role	EC contrib. [€]
1	MAX-PLANCK-GESELLSCHAFT ZUR FORDERUNG DER WISSENSCHAFTEN EV MAX-PLANCK-GESELLSCHAFT ZUR FORDERUNG DER WISSENSCHAFTEN EV Organization address address: HOFGARTENSTRASSE 8 city: Munich postcode: 80539 website: www.mpg.de contact info title: n.a. name: n.a. surname: n.a. function: n.a. email: n.a. telephone: n.a. fax: n.a.	DE (Munich)	coordinator	159˙460.00

Map

Project objective

The ability of cells to engulf large objects, such as invading microorganisms or apoptotic cells, is crucial to innate immunity and tissue remodelling. The molecular basis of this process - phagocytosis - is complex, involving numerous receptors and signalling pathways. Nevertheless, the biophysical process is always the same: the cell membrane deforms and reshapes to wrap around the particle, and upon closure and abscission of the resultant cup, the particle is internalised. Although the key molecular players in individual phagocytic pathways have been identified, we still know very little about the basic biophysics common to all phagocytic pathways. I propose to fill this gap in our knowledge by creating a “minimal phagocyte”: I aim to reconstitute a minimal, dynamic actin cytoskeleton and artificial phagocytic receptors in giant unilamellar vesicles (GUVs), thereby identifying the molecular components that are not only necessary but also sufficient for phagocytosis. Using synthetic biology to build a bottom-up model of phagocytosis should answer many open questions, including: are spatial cues resulting from particle binding required for membrane wrapping around the particle? Is directed initiation of actin polymerisation sufficient to render GUVs capable of phagocytosis? What is the role of the membrane-supporting actin cortex and how does the affinity of the receptors affect the engulfment process? Beyond phagocytosis, the minimal-model approach I propose will also be useful to study other cellular functions requiring actin-driven membrane reorganisation, such as cell mobility. In line with the objectives set by ERA-NET ERASynBio and the Horizon 2020 work programme (which identified synthetic biology as one of the “cutting-edge biotechnologies as future innovation drivers”), the creation of “protocells” will not only enhance our understanding of biology, but ultimately also result in novel biotechnological applications, such as improved drug delivery systems.

Publications

List of publications.
year	authors and title	journal	last update
2018	Thomas Litschel, Kristina A Ganzinger, Torgeir Movinkel, Michael Heymann, Tom Robinson, Hannes Mutschler, Petra Schwille Freeze-thaw cycles induce content exchange between cell-sized lipid vesicles published pages: 55008, ISSN: 1367-2630, DOI: 10.1088/1367-2630/aabb96	New Journal of Physics 20/5	2019-06-13

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