#	Pagina
attuale pagina	/open-h2020/projects/201108/index.html

Opendata, web and dolomites

minimal-phagocyte SIGNED

Reconstitution of the basic molecular mechanism of phagocytosis – a bottom-up synthetic biology approach

Total Cost €

EC-Contrib. €

Partnership

Views

Outcomes and
results

minimal-phagocyte project word cloud

Explore the words cloud of the minimal-phagocyte project. It provides you a very rough idea of what is the project "minimal-phagocyte" about.

ant biotechnological thereby protocells fill deforms ing nevertheless reorganisation biophysics innovation render gap cell erasynbio directed actin initiation ultimately creation remodelling always spatial cup polymerisation cytoskeleton innate signalling internalised receptors giant cortex wrap microorganisms dynamic engulfment net line cutting drivers phagocytosis guvs supporting era affinity edge molecular functions requiring horizon questions particle players 2020 process individual phagocytic answer synthetic wrapping minimal phagocyte drug little components cellular basis immunity closure model abscission biotechnologies cues tissue biology engulf biophysical artificial binding objects basic unilamellar mobility sufficient cells apoptotic invading reconstitute reshapes membrane vesicles

Project "minimal-phagocyte" data sheet

The following table provides information about the project.

Coordinator	MAX-PLANCK-GESELLSCHAFT ZUR FORDERUNG DER WISSENSCHAFTEN EV Organization address address: HOFGARTENSTRASSE 8 city: Munich postcode: 80539 website: www.mpg.de contact info title: n.a. name: n.a. surname: n.a. function: n.a. email: n.a. telephone: n.a. fax: n.a.
Coordinator Country	Germany [DE]
Total cost	159˙460 €
EC max contribution	159˙460 € (100%)
Programme	1. H2020-EU.1.3.2. (Nurturing excellence by means of cross-border and cross-sector mobility)
Code Call	H2020-MSCA-IF-2015
Funding Scheme	MSCA-IF-EF-ST
Starting year	2016
Duration (year-month-day)	from 2016-09-01 to 2018-08-31

Partnership

Take a look of project's partnership.

#	participants	country	role	EC contrib. [€]
1	MAX-PLANCK-GESELLSCHAFT ZUR FORDERUNG DER WISSENSCHAFTEN EV MAX-PLANCK-GESELLSCHAFT ZUR FORDERUNG DER WISSENSCHAFTEN EV Organization address address: HOFGARTENSTRASSE 8 city: Munich postcode: 80539 website: www.mpg.de contact info title: n.a. name: n.a. surname: n.a. function: n.a. email: n.a. telephone: n.a. fax: n.a.	DE (Munich)	coordinator	159˙460.00

Map

Project objective

The ability of cells to engulf large objects, such as invading microorganisms or apoptotic cells, is crucial to innate immunity and tissue remodelling. The molecular basis of this process - phagocytosis - is complex, involving numerous receptors and signalling pathways. Nevertheless, the biophysical process is always the same: the cell membrane deforms and reshapes to wrap around the particle, and upon closure and abscission of the resultant cup, the particle is internalised. Although the key molecular players in individual phagocytic pathways have been identified, we still know very little about the basic biophysics common to all phagocytic pathways. I propose to fill this gap in our knowledge by creating a “minimal phagocyte”: I aim to reconstitute a minimal, dynamic actin cytoskeleton and artificial phagocytic receptors in giant unilamellar vesicles (GUVs), thereby identifying the molecular components that are not only necessary but also sufficient for phagocytosis. Using synthetic biology to build a bottom-up model of phagocytosis should answer many open questions, including: are spatial cues resulting from particle binding required for membrane wrapping around the particle? Is directed initiation of actin polymerisation sufficient to render GUVs capable of phagocytosis? What is the role of the membrane-supporting actin cortex and how does the affinity of the receptors affect the engulfment process? Beyond phagocytosis, the minimal-model approach I propose will also be useful to study other cellular functions requiring actin-driven membrane reorganisation, such as cell mobility. In line with the objectives set by ERA-NET ERASynBio and the Horizon 2020 work programme (which identified synthetic biology as one of the “cutting-edge biotechnologies as future innovation drivers”), the creation of “protocells” will not only enhance our understanding of biology, but ultimately also result in novel biotechnological applications, such as improved drug delivery systems.

Publications

List of publications.
year	authors and title	journal	last update
2018	Thomas Litschel, Kristina A Ganzinger, Torgeir Movinkel, Michael Heymann, Tom Robinson, Hannes Mutschler, Petra Schwille Freeze-thaw cycles induce content exchange between cell-sized lipid vesicles published pages: 55008, ISSN: 1367-2630, DOI: 10.1088/1367-2630/aabb96	New Journal of Physics 20/5	2019-06-13

Are you the coordinator (or a participant) of this project? Plaese send me more information about the "MINIMAL-PHAGOCYTE" project.

For instance: the website url (it has not provided by EU-opendata yet), the logo, a more detailed description of the project (in plain text as a rtf file or a word file), some pictures (as picture files, not embedded into any word file), twitter account, linkedin page, etc.

Send me an email (fabio@fabiodisconzi.com) and I put them in your project's page as son as possible.

Thanks. And then put a link of this page into your project's website.

The information about "MINIMAL-PHAGOCYTE" are provided by the European Opendata Portal: CORDIS opendata.