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3DQuant

Understanding long-range transcriptional regulation in the context of the 3D genome organization

Total Cost €

0

EC-Contrib. €

0

Partnership

0

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 3DQuant project word cloud

Explore the words cloud of the 3DQuant project. It provides you a very rough idea of what is the project "3DQuant" about.

isolated    gene    topologically    genomic    view    communication    architecture    cognate    linked    spatial    unravel    single    tads    influences    sub    genome    mouse    proximity    outputs    modulation    relationship    contributes    stem    genetic    folded    regulatory    environment    temporal    mobilized    cells    3d    transcriptional    close    engineered    distances    unknown    chromatin    partitioning    establishment    measured    promoters    cell    shown    expression    imaging    unprecedented    chromosome    modulate    chromosomes    conformation    generate    biophysical    engineering    megabase    capture    underlying    quantitatively    self    mediated    associating    partitioned    space    promoter    dimensional    technologies    mechanism    modulates    regulation    pairs    mechanisms    correlated    physical    enhancers    suggests    quantitative    techniques    genes    transcription    action    measuring    interactions    3c    organization    loops    structure    metazoans    manner    patterns    embryonic    precisely    enhancer    live    domains    mammalian   

Project "3DQuant" data sheet

The following table provides information about the project.

Coordinator
FRIEDRICH MIESCHER INSTITUTE FOR BIOMEDICAL RESEARCH FONDATION 

Organization address
address: MAULBEERSTRASSE 66
city: BASEL
postcode: 4058
website: www.fmi.ch

contact info
title: n.a.
name: n.a.
surname: n.a.
function: n.a.
email: n.a.
telephone: n.a.
fax: n.a.

 Coordinator Country Switzerland [CH]
 Total cost 175˙419 €
 EC max contribution 175˙419 € (100%)
 Programme 1. H2020-EU.1.3.2. (Nurturing excellence by means of cross-border and cross-sector mobility)
 Code Call H2020-MSCA-IF-2016
 Funding Scheme MSCA-IF-EF-ST
 Starting year 2017
 Duration (year-month-day) from 2017-03-01   to  2019-02-28

 Partnership

Take a look of project's partnership.

# participants  country  role  EC contrib. [€] 
1    FRIEDRICH MIESCHER INSTITUTE FOR BIOMEDICAL RESEARCH FONDATION CH (BASEL) coordinator 175˙419.00

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 Project objective

Enhancers are regulatory elements that control the spatial and temporal expression of genes in metazoans. Enhancers are able to modulate transcription of a target gene from large genomic distances, as a result of the formation of chromatin loops that bring them in close spatial proximity to cognate promoters. The manner how specific patterns of enhancer-promoter physical interactions are established is linked to how chromosomes are folded in the three-dimensional (3D) space. Recent studies based on chromosome conformation capture (3C) have shown that mammalian chromosomes are partitioned into self-associating sub-megabase domains called Topologically Associating Domains (TADs). Genetic evidence suggests that 3D chromatin organization within and across TADs contributes to the establishment and partitioning of enhancers-promoters physical communication. Yet it is still unknown by which biophysical mechanisms chromosome architecture modulates enhancer action, and thus transcription. The goal of this proposal is to determine the quantitative relationship between 3D chromatin architecture and enhancer-promoter activity to unravel the mechanism of long-range transcriptional modulation mediated by enhancers. Addressing this goal requires a system where transcriptional outputs can be measured precisely and quantitatively, and correlated with 3D distances. To this aim, we will use state-of-the art genome engineering techniques to generate mouse embryonic stem cells with engineered enhancer-promoter pairs in an isolated chromatin environment, where a selected enhancer can be mobilized at different distances from its cognate promoter. We will use this system to quantitatively assess how 3D chromatin structure influences enhancer action by measuring transcription and promoter-enhancer interactions using 3C-based technologies, single-cell methods and live-cell imaging. This will lead to an unprecedented view of the mechanisms underlying long-range transcriptional regulation.

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