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HighThroughFROGS SIGNED

Reviving ghosts of taxonomy past: Identifying cryptic species using high-throughput sequencing of historical museum specimens for Asian ranids with gastromyzophorous tadpoles

Total Cost €

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EC-Contrib. €

0

Partnership

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 HighThroughFROGS project word cloud

Explore the words cloud of the HighThroughFROGS project. It provides you a very rough idea of what is the project "HighThroughFROGS" about.

apart    systematics    advancing    critical    ranid    dna    group    degraded    sequence    clarification    species    retrieving    tropical    techniques    decades    asian    raw    taxa    genome    tools    valuable    obtaining    phylogenetics    exome    my    marie    historical    postdoctoral    actions    samples    museums    2020    collected    career    sequencing    obtain    tadpoles    genetic    first    tropics    quantifying    materials    baseline    countries    housed    gastromyzophory    biogeographic    phylogenetic    actively    disseminate    protocols    cryptic    usa    hts    exceptional    designed    fragment    frogs    evolution    aligned    curie    freshly    sklodowska    sanger    catalyst    tested    modern    taxonomic    independent    capture    tissue    phylogenomic    gastromyzophorous    data    conservation    indonesia    specimens    ago    se    genomic    emphasize    molecular    defied    feasibility    diversity    audience    myself    position    natural    science    mtdna    play    date    horizon    germany    speciation    biodiversity    museum    focal    researcher    collaborations    history   

Project "HighThroughFROGS" data sheet

The following table provides information about the project.

Coordinator		 UNIVERSITAET HAMBURG 

Organization address
address: MITTELWEG 177
city: HAMBURG
postcode: 20148
website: n.a.

contact info
title: n.a.
name: n.a.
surname: n.a.
function: n.a.
email: n.a.
telephone: n.a.
fax: n.a.
 Coordinator Country Germany [DE]
 Total cost 246˙669 €
 EC max contribution 246˙669 € (100%)
 Programme 1. H2020-EU.1.3.2. (Nurturing excellence by means of cross-border and cross-sector mobility)
 Code Call H2020-MSCA-IF-2018
 Funding Scheme MSCA-IF-GF
 Starting year 2020
 Duration (year-month-day) from 2020-03-01   to  2023-02-28

 Partnership

Take a look of project's partnership.

# participants  country  role  EC contrib. [€] 
1    UNIVERSITAET HAMBURG DE (HAMBURG) coordinator 246˙669.00
2    THE REGENTS OF THE UNIVERSITY OF CALIFORNIA US (OAKLAND CA) partner 0.00

Map

 Project objective

My postdoctoral study is designed to develop protocols and procedures to obtain genomic DNA data from degraded old museum specimens using HTS (e.g., exome-capture and/ or mtDNA genome sequencing) using SE Asian frogs as focal taxa. Specimens housed in natural history museums are essential raw materials for studies of cryptic speciation that is very common in the tropics. These studies require molecular genetic tools that, to date, could only be applied to freshly collected tissue samples. Because HTS is based on short-fragment sequencing technology, it is more effective at obtaining sequence data from historical specimens than Sanger sequencing, particularly for very old samples collected more than 100 years ago. HTS methods will be tested to assess the phylogenetic and taxonomic position of the Asian ranid frogs with gastromyzophorous tadpoles, a group that has defied taxonomic clarification for decades. This proposed study is well aligned to the Work Programme of Horizon 2020 Marie Sklodowska-Curie Actions. The results of this study will be a valuable baseline to assess the feasibility of and to improve exome-capture techniques aimed at retrieving historical DNA from type specimens for future studies. This study will be the first to investigate the evolution of gastromyzophory in Asian ranid frogs. It will also emphasize the need to apply modern techniques in tropical countries with high biodiversity as a critical step in quantifying its diversity and developing conservation actions for cryptic species. Apart from that, this study is an exceptional step towards advancing my career and establish myself as an independent researcher in the field of phylogenetics systematics. Furthermore, I will be able to actively contribute to disseminate my science to broader audience and play an important role as a catalyst for a long term collaborations between the institutions in Germany, USA, and Indonesia in phylogenomic, systematics, and biogeographic studies.

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The information about "HIGHTHROUGHFROGS" are provided by the European Opendata Portal: CORDIS opendata.

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