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SCSC SIGNED
The molecular diversity of regeneration in the zebrafish spinal cord
Total Cost €
0EC-Contrib. €
0Partnership
0Views
0SCSC project word cloud
Explore the words cloud of the SCSC project. It provides you a very rough idea of what is the project "SCSC" about.
capacity
genome
laboratory
data
mammalian
unable
exceptional
instruct
types
yield
forefront
limited
rna
cell
independent
signals
nervous
cords
localize
expression
transcriptional
cord
gap
neuroscience
humans
contrast
exhibits
training
causes
taxonomy
day
unclear
throughput
isolating
seq
bulk
experimental
biology
candidates
nuclei
entails
elucidate
single
molecular
profiling
regenerating
preparing
permanent
career
roles
revealed
diversity
resource
microarray
regenerative
spinal
zebrafish
specializes
nucleus
analyzing
strategies
host
performing
gene
regulate
because
computational
protocol
suggest
atlas
explored
reveal
damage
postdoctoral
injury
regeneration
investigator
regenerate
Project "SCSC" data sheet
The following table provides information about the project.
| Coordinator |
KAROLINSKA INSTITUTET
Organization address contact info |
| Coordinator Country | Sweden [SE] |
| Total cost | 191˙852 € |
| EC max contribution | 191˙852 € (100%) |
| Programme |
1. H2020-EU.1.3.2. (Nurturing excellence by means of cross-border and cross-sector mobility) |
| Code Call | H2020-MSCA-IF-2018 |
| Funding Scheme | MSCA-IF-EF-ST |
| Starting year | 2020 |
| Duration (year-month-day) | from 2020-08-01 to 2022-07-31 |
Partnership
Take a look of project's partnership.
| # | ||||
|---|---|---|---|---|
| 1 | KAROLINSKA INSTITUTET | SE (STOCKHOLM) | coordinator | 191˙852.00 |
Map
Project objective
Because the mammalian nervous system exhibits a limited capacity for regeneration, spinal-cord injury causes permanent damage in humans. In contrast, zebrafish regenerate their spinal cords after damage. Understanding this process in zebrafish might one day suggest regenerative strategies for humans. This proposal therefore aims to elucidate the transcriptional processes that regulate regeneration in the zebrafish spinal cord. Although genome-wide expression profiling has revealed factors that promote spinal cord regeneration, microarray and bulk RNA-seq methods are unable to localize candidates to particular cell types. The molecular diversity of cell types that contribute to spinal cord regeneration thus has not been fully explored, and the cell type-specific roles of the signals that instruct the process remain unclear. The applicant will address this gap by performing single-nucleus RNA-seq on regenerating zebrafish spinal cord and analyzing the data to reveal cell types and cell type-specific gene expression changes. This goal entails three specific aims: 1) Establish a protocol for isolating nuclei from the zebrafish spinal cord. 2) Establish a molecular taxonomy of cell types in the regenerating spinal cord. 3) Characterize cell type-specific gene-expression changes in the regenerating spinal cord. The project will be completed in a laboratory that specializes in single-cell RNA-seq methods and neuroscience, providing exceptional training in the experimental and computational methods associated with high-throughput single-cell analysis and preparing the applicant for a career as an independent investigator. The host institute is moreover a leading research institute at the forefront of neuroscience and single-cell biology, with postdoctoral career support. The proposal will yield an atlas of the cell type-specific transcriptional changes that occur during regeneration, providing new insight and a novel resource for researchers in the field of regeneration.
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No valid XML
lastchecktime (2024-05-08 20:39:26) correctly updated