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PrOBiEM SIGNED

Characterization of the anaerobic degradation of phenanthrene by a novel sulfate-reducing enrichment culture for the development of bioremediation methods

Total Cost €

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EC-Contrib. €

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Partnership

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Project "PrOBiEM" data sheet

The following table provides information about the project.

Coordinator
UNIVERSITAET DUISBURG-ESSEN 

Organization address
address: UNIVERSITATSSTRASSE 2
city: ESSEN
postcode: 45141
website: www.uni-duisburg-essen.de

contact info
title: n.a.
name: n.a.
surname: n.a.
function: n.a.
email: n.a.
telephone: n.a.
fax: n.a.

 Coordinator Country Germany [DE]
 Project website https://www.uni-due.de/biofilm-centre/mikro_projekte_probiem.php
 Total cost 159˙460 €
 EC max contribution 159˙460 € (100%)
 Programme 1. H2020-EU.1.3.2. (Nurturing excellence by means of cross-border and cross-sector mobility)
 Code Call H2020-MSCA-IF-2015
 Funding Scheme MSCA-IF-EF-ST
 Starting year 2016
 Duration (year-month-day) from 2016-06-02   to  2018-06-01

 Partnership

Take a look of project's partnership.

# participants  country  role  EC contrib. [€] 
1    UNIVERSITAET DUISBURG-ESSEN DE (ESSEN) coordinator 159˙460.00

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 Project objective

Polycyclic aromatic hydrocarbons (PAHs) are widely distributed contaminants that produce hazardous effects on human health. They are persistent in the environment and recalcitrant to degradation, rendering the development of efficient PAH-removal methods a worldwide concern. In PAH-contaminated aquifers or sediments, oxygen is depleted very rapidly, thus microorganisms able to use these compounds as a carbon source in the absence of molecular oxygen are crucial for their consumption. The aim of this project is to elucidate the enzymes and mechanisms for the anaerobic degradation of phenanthrene by a novel sulfate-reducing enrichment culture. The first objective is to identify the organisms responsible for phenanthrene degradation. A phylogenetic characterization will be performed by pyrosequencing of the 16S rRNA genes amplified from the enrichment culture. The second objective is to discover the enzymes involved in the degradation process. To this end, a combined transcriptomic and proteomic evaluation of the enrichment culture will be performed. The functionality of the identified enzymes will be then evaluated in vitro. The chemical stability and complicated structure of phenanthrene promises the discovery of novel types of enzymatic reactions. Finally, the microorganisms responsible for phenanthrene degradation will be purified to homogeneity and characterized to evaluate the optimal environmental conditions for growth and phenanthrene degradation. Options to improve biodegradation at contaminated sites and to promote phenanthrene removal will be studied. The project proposes insights into the anaerobic biodegradation of three-ring PAHs as one of the most prominent classes of environmental contaminants.

 Publications

year authors and title journal last update
List of publications.
2018 Ivana Kraiselburd, Thomas Bruls, Anne M. Himmelberg, Rainer U. Meckenstock
Anaerobic degradation of phenanthrene by a novel sulfate-reducing bacterial enrichment culture
published pages: , ISSN: , DOI:
VAAM 2018 Book of Abstracts 2019-06-13
2017 Ivana Kraiselburd, Thomas Bruls, Rainer U. Meckenstock
Characterization of the anaerobic degradation of phenanthrene by a novel sulfate-reducing bacterial enrichment culture
published pages: , ISSN: , DOI:
EMBO Book of Abstracts 2019-06-13
2017 Ivana Kraiselburd, Thomas Bruls, Rainer U. Meckenstock
Anaerobic degradation of phenanthrene by a novel sulfate-reducing bacterial enrichment culture
published pages: , ISSN: , DOI:
ISSM 2018 Book of Abstracts 2019-06-13
2017 Ivana Kraiselburd, Thomas Bruls, Rainer U. Meckenstock
Characterization of the anaerobic degradation of phenanthrene by a novel sulfate-reducing bacterial enrichment culture
published pages: , ISSN: , DOI:
FEMS 2017 Book of Abstracts 2019-06-13
2018 Ivana Kraiselburd, Thomas Bruls, Rainer U. Meckenstock
Anaerobic degradation of phenanthrene by a novel sulfate-reducing bacterial enrichment culture
published pages: , ISSN: , DOI:
2019-06-13
2017 Ivana Kraiselburd, Thomas Bruls, Rainer U. Meckenstock
Characterization of the anaerobic degradation of phenanthrene by a novel sulfate-reducing bacterial enrichment culture
published pages: , ISSN: , DOI:
2019-06-13

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The information about "PROBIEM" are provided by the European Opendata Portal: CORDIS opendata.

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