Opendata, web and dolomites


Biochemical and CryoEM studies of spliceosome activation.

Total Cost €


EC-Contrib. €






 SPLICEOSACT project word cloud

Explore the words cloud of the SPLICEOSACT project. It provides you a very rough idea of what is the project "SPLICEOSACT" about.

eukaryotic    catalytically    thanks    stabilization    cryoem    resolution    conformational    recruited    solved    u2    details    perform    molecular    assembled    yeast    discrete    combination    u4    successive    introns    competent    microscopy    group    gigantic    maturation    mrnas    technological    stepwise    removal    characterization    particle    reactions    host    unraveling    rna    earlier    active    novo    catalytic    regarding    created    activation    single    gap    stages    structures    undergoes    biological    catalysis    lost    reactive    consists    u5    formed    consisting    atomic    uses    mechanistic    manner    em    concomitant    excitement    u6    intron    u1    community    visualize    mrna    groups    catalyzed    site    biochemical    cycle    series    splicing    spliceosomes    complexes    transesterification    transition    questions    exons    filling    centre    scarcity    spliceosome    correct    snrnps    compositional    extensive    de    assembles    positioning    fundamental    electron    captured    ribonucleoprotein    ligation    pre   

Project "SPLICEOSACT" data sheet

The following table provides information about the project.


There are not information about this coordinator. Please contact Fabio for more information, thanks.

 Coordinator Country United Kingdom [UK]
 Total cost 195˙454 €
 EC max contribution 195˙454 € (100%)
 Programme 1. H2020-EU.1.3.2. (Nurturing excellence by means of cross-border and cross-sector mobility)
 Code Call H2020-MSCA-IF-2017
 Funding Scheme MSCA-IF-EF-ST
 Starting year 2019
 Duration (year-month-day) from 2019-03-01   to  2021-02-28


Take a look of project's partnership.

# participants  country  role  EC contrib. [€] 


 Project objective

Pre-mRNA splicing is a fundamental maturation step of eukaryotic mRNAs that consists of the removal of introns and the concomitant ligation of exons by two successive transesterification reactions. This complex biological process is catalyzed by the spliceosome, a gigantic ribonucleoprotein particle that assembles de novo on each intron and uses a single RNA-based active site to perform both reactions. The spliceosome is composed of five snRNPs (U1, U2, U4, U5, U6) that are recruited to pre-mRNAs in a stepwise manner. When a pre-catalytic spliceosome, consisting of all five snRNPs, is formed on a pre-mRNA it has no pre-existing active site and undergoes extensive compositional and conformational changes to become “catalytically competent”. During this transition, two snRNPs (U1 and U4) are lost and several new factors are recruited, ensuring the formation and stabilization of the active site as well as the correct positioning of the pre-mRNA’s reactive groups in the catalytic centre. Thanks to recent technological advances in Electron Microscopy (EM), a series of cryoEM structures of fully assembled “active” spliceosomes at atomic resolution have been solved in the host group and elsewhere, in the past two years. These structures, which have created much excitement in the RNA community, visualize the spliceosome during each step of the catalytic cycle and allow a mechanistic understanding of catalysis. However, due to the scarcity of high-resolution information on earlier complexes, many questions remain regarding spliceosome activation. My project aims at filling a gap in our understanding of pre-mRNA splicing by unraveling the molecular details of spliceosome activation. To that end, I will use a combination of biochemical characterization and cryoEM to study yeast spliceosome captured at discrete early stages of activation.

Are you the coordinator (or a participant) of this project? Plaese send me more information about the "SPLICEOSACT" project.

For instance: the website url (it has not provided by EU-opendata yet), the logo, a more detailed description of the project (in plain text as a rtf file or a word file), some pictures (as picture files, not embedded into any word file), twitter account, linkedin page, etc.

Send me an  email ( and I put them in your project's page as son as possible.

Thanks. And then put a link of this page into your project's website.

The information about "SPLICEOSACT" are provided by the European Opendata Portal: CORDIS opendata.

More projects from the same programme (H2020-EU.1.3.2.)

RETHEIR (2019)

The return of the heirs

Read More  

OCHRE (2019)

Oat CHRomosome Evolution and drivers enabling widespread terminal intergenomic translocations in polyploid species

Read More  

EstAMR (2020)

Estimating the Prevalence of AntiMicrobial Resistance

Read More