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SPLICEOSACT SIGNED

Biochemical and CryoEM studies of spliceosome activation.

Total Cost €

0

EC-Contrib. €

0

Partnership

0

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 SPLICEOSACT project word cloud

Explore the words cloud of the SPLICEOSACT project. It provides you a very rough idea of what is the project "SPLICEOSACT" about.

activation    biological    reactive    formed    stabilization    assembles    spliceosomes    resolution    community    cryoem    created    ribonucleoprotein    host    stepwise    exons    discrete    manner    extensive    combination    lost    filling    excitement    thanks    ligation    removal    u2    consists    gap    electron    successive    catalytic    de    unraveling    regarding    introns    correct    solved    splicing    yeast    molecular    eukaryotic    reactions    mechanistic    em    competent    site    perform    maturation    active    complexes    intron    single    u1    mrna    details    fundamental    positioning    spliceosome    recruited    visualize    snrnps    stages    transesterification    novo    catalysis    technological    particle    rna    group    earlier    conformational    scarcity    captured    atomic    structures    centre    undergoes    microscopy    uses    pre    characterization    questions    gigantic    assembled    compositional    u6    catalytically    transition    u5    u4    concomitant    groups    consisting    series    mrnas    biochemical    catalyzed    cycle   

Project "SPLICEOSACT" data sheet

The following table provides information about the project.

Coordinator
UNITED KINGDOM RESEARCH AND INNOVATION 

There are not information about this coordinator. Please contact Fabio for more information, thanks.

 Coordinator Country United Kingdom [UK]
 Total cost 195˙454 €
 EC max contribution 195˙454 € (100%)
 Programme 1. H2020-EU.1.3.2. (Nurturing excellence by means of cross-border and cross-sector mobility)
 Code Call H2020-MSCA-IF-2017
 Funding Scheme MSCA-IF-EF-ST
 Starting year 2019
 Duration (year-month-day) from 2019-03-01   to  2021-02-28

 Partnership

Take a look of project's partnership.

# participants  country  role  EC contrib. [€] 
1    UNITED KINGDOM RESEARCH AND INNOVATION UK (SWINDON) coordinator 195˙454.00
2    MEDICAL RESEARCH COUNCIL UK (SWINDON) coordinator 0.00

Map

 Project objective

Pre-mRNA splicing is a fundamental maturation step of eukaryotic mRNAs that consists of the removal of introns and the concomitant ligation of exons by two successive transesterification reactions. This complex biological process is catalyzed by the spliceosome, a gigantic ribonucleoprotein particle that assembles de novo on each intron and uses a single RNA-based active site to perform both reactions. The spliceosome is composed of five snRNPs (U1, U2, U4, U5, U6) that are recruited to pre-mRNAs in a stepwise manner. When a pre-catalytic spliceosome, consisting of all five snRNPs, is formed on a pre-mRNA it has no pre-existing active site and undergoes extensive compositional and conformational changes to become “catalytically competent”. During this transition, two snRNPs (U1 and U4) are lost and several new factors are recruited, ensuring the formation and stabilization of the active site as well as the correct positioning of the pre-mRNA’s reactive groups in the catalytic centre. Thanks to recent technological advances in Electron Microscopy (EM), a series of cryoEM structures of fully assembled “active” spliceosomes at atomic resolution have been solved in the host group and elsewhere, in the past two years. These structures, which have created much excitement in the RNA community, visualize the spliceosome during each step of the catalytic cycle and allow a mechanistic understanding of catalysis. However, due to the scarcity of high-resolution information on earlier complexes, many questions remain regarding spliceosome activation. My project aims at filling a gap in our understanding of pre-mRNA splicing by unraveling the molecular details of spliceosome activation. To that end, I will use a combination of biochemical characterization and cryoEM to study yeast spliceosome captured at discrete early stages of activation.

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The information about "SPLICEOSACT" are provided by the European Opendata Portal: CORDIS opendata.

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