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SPLICEOSACT SIGNED

Biochemical and CryoEM studies of spliceosome activation.

Total Cost €

0

EC-Contrib. €

0

Partnership

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 SPLICEOSACT project word cloud

Explore the words cloud of the SPLICEOSACT project. It provides you a very rough idea of what is the project "SPLICEOSACT" about.

maturation    perform    uses    resolution    technological    manner    activation    created    splicing    cycle    atomic    u4    stabilization    combination    single    regarding    groups    series    recruited    particle    correct    transesterification    u1    pre    removal    excitement    consisting    spliceosomes    exons    conformational    extensive    community    gigantic    assembles    questions    novo    active    introns    captured    structures    catalytic    consists    thanks    eukaryotic    cryoem    compositional    transition    mrna    mechanistic    formed    details    ribonucleoprotein    snrnps    intron    yeast    biological    reactive    biochemical    unraveling    rna    reactions    competent    solved    filling    stages    mrnas    u6    visualize    catalyzed    electron    earlier    discrete    de    undergoes    catalytically    gap    concomitant    site    u2    stepwise    microscopy    host    group    fundamental    molecular    spliceosome    ligation    assembled    characterization    centre    successive    catalysis    scarcity    positioning    lost    complexes    em    u5   

Project "SPLICEOSACT" data sheet

The following table provides information about the project.

Coordinator
UNITED KINGDOM RESEARCH AND INNOVATION 

There are not information about this coordinator. Please contact Fabio for more information, thanks.

 Coordinator Country United Kingdom [UK]
 Total cost 195˙454 €
 EC max contribution 195˙454 € (100%)
 Programme 1. H2020-EU.1.3.2. (Nurturing excellence by means of cross-border and cross-sector mobility)
 Code Call H2020-MSCA-IF-2017
 Funding Scheme MSCA-IF-EF-ST
 Starting year 2019
 Duration (year-month-day) from 2019-03-01   to  2021-02-28

 Partnership

Take a look of project's partnership.

# participants  country  role  EC contrib. [€] 
1    UNITED KINGDOM RESEARCH AND INNOVATION UK (SWINDON) coordinator 195˙454.00
2    MEDICAL RESEARCH COUNCIL UK (SWINDON) coordinator 0.00

Map

 Project objective

Pre-mRNA splicing is a fundamental maturation step of eukaryotic mRNAs that consists of the removal of introns and the concomitant ligation of exons by two successive transesterification reactions. This complex biological process is catalyzed by the spliceosome, a gigantic ribonucleoprotein particle that assembles de novo on each intron and uses a single RNA-based active site to perform both reactions. The spliceosome is composed of five snRNPs (U1, U2, U4, U5, U6) that are recruited to pre-mRNAs in a stepwise manner. When a pre-catalytic spliceosome, consisting of all five snRNPs, is formed on a pre-mRNA it has no pre-existing active site and undergoes extensive compositional and conformational changes to become “catalytically competent”. During this transition, two snRNPs (U1 and U4) are lost and several new factors are recruited, ensuring the formation and stabilization of the active site as well as the correct positioning of the pre-mRNA’s reactive groups in the catalytic centre. Thanks to recent technological advances in Electron Microscopy (EM), a series of cryoEM structures of fully assembled “active” spliceosomes at atomic resolution have been solved in the host group and elsewhere, in the past two years. These structures, which have created much excitement in the RNA community, visualize the spliceosome during each step of the catalytic cycle and allow a mechanistic understanding of catalysis. However, due to the scarcity of high-resolution information on earlier complexes, many questions remain regarding spliceosome activation. My project aims at filling a gap in our understanding of pre-mRNA splicing by unraveling the molecular details of spliceosome activation. To that end, I will use a combination of biochemical characterization and cryoEM to study yeast spliceosome captured at discrete early stages of activation.

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The information about "SPLICEOSACT" are provided by the European Opendata Portal: CORDIS opendata.

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