MYCAP

"DEVELOPMENT OF A TECHNOLOGY TO PRODUCE MICROCAPSULES, based on the formation of drops from viscous non-Newtonian liquids sprayed through fan-jet nozzles, TO USE IN CANCER THERAPY"

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 Obiettivo del progetto (Objective)

'The main aim of this project is the development of a new technology to produce smart drug delivery system for chemotherapeutic agents per recognition event. For that affinity-microparticles (10-20 microns diameter) loaded of paclitaxel (PTX) or Endotastin will be produced. Microcapsules will be made using a new technology based on the formation of drops from viscous non- Newtonian liquids sprayed through fan-jet nozzles. This process is based on generation of kinetic energy to a liquid jet resulting on controlled spray generation. The technique will be modelled in order to ensure the scale-up the process. The microparticles, based on alginate polymer, will be functionalised on his surface by affinity ligand, epidermal growth factor (EGF) which will be able to recognize a specific protein of the tumoral cell, (EGFR) epidermal growth factor receptor. Surface plasma resonance will be carried to control the interaction between the microparticle and the protein and therefore to ensure the efficiency of the microparticles produced. This information will be used to developed a dynamic model to assess the importance of spatial phenomena and then we will evaluate the accuracy of partial differential equations (PDEs) in transient when spatial effects are important. Control release from microcapsules loaded of anticancer agent will be characterized by control release kinetics, mass transfer, mechanic stability and permeability studies. Mass transfer through the tissue, or therapeutic leakage from storage cavities and their consequent transport through the organ, are among the several physical processes, where knowledge of the unsteady transport of a scalar quantity (mass of an active) is of importance for cell therapy. For that reasons it is necessary to derive an analytical solution for the unsteady mass transport problem in a porous medium under torsional flow to simulate the diffusion of active materials in body cavities (Mixed mechanic-electrical model), assuming body cavities as ideally isotropic porous medium. Finally, characterized microcapsules will be tested in lung tissues with lung cancer. Cell viability (MTT) and apoptosis after PTX exposure in non small cell lung cancer (NSCLC) will be studied. Morphological distribution of particles in areas of interest (lung, pleura and lymph nodes) will be examined. The experimental results found in vitro will compared with experimental animal models developed for tumoral cell death.'