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CHROMAVISION SIGNED

Super-resolution visualisation and manipulation of metaphase chromosomes

Total Cost €

EC-Contrib. €

Partnership

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Outcomes and
results

CHROMAVISION project word cloud

Explore the words cloud of the CHROMAVISION project. It provides you a very rough idea of what is the project "CHROMAVISION" about.

delivered structural cells time etiology trap molecular small combine chip mechanisms disorders isolation isolate fertility human potentially decades ctfm chromavision diseases chromosome errors complexity 3d diseased impaired tissue fuel syndrome technique powerful microfluidics physiological tweezers humanity observation chromosomal instrument correlative separate sr3d biologists samples push manipulation super chromosomes ing opto discovery single maternal clinical metaphase microscopy boundaries drug cancer discoveries lysate pioneering division individual aging fluidic identification understand imaging fragile heterogeneity fundamental automatically platform healthy aid lyse first fluorescence breakthrough resolution zoom visualise abnormalities near monitoring neurological microscope cell

Project "CHROMAVISION" data sheet

The following table provides information about the project.

Coordinator	STICHTING VU Organization address address: DE BOELELAAN 1105 city: AMSTERDAM postcode: 1081 HV website: www.vu.nl contact info title: n.a. name: n.a. surname: n.a. function: n.a. email: n.a. telephone: n.a. fax: n.a.
Coordinator Country	Netherlands [NL]
Project website	http://www.chromavision.eu
Total cost	3˙567˙025 €
EC max contribution	3˙567˙025 € (100%)
Programme	1. H2020-EU.1.2.1. (FET Open)
Code Call	H2020-FETOPEN-2014-2015-RIA
Funding Scheme	RIA
Starting year	2015
Duration (year-month-day)	from 2015-06-01 to 2019-05-31

Partnership

Take a look of project's partnership.

#	participants	country	role	EC contrib. [€]
1	STICHTING VU STICHTING VU Organization address address: DE BOELELAAN 1105 city: AMSTERDAM postcode: 1081 HV website: www.vu.nl contact info title: n.a. name: n.a. surname: n.a. function: n.a. email: n.a. telephone: n.a. fax: n.a.	NL (AMSTERDAM)	coordinator	1˙054˙003.00
2	LUMICKS B.V. LUMICKS B.V. Organization address address: DE BOELELAAN 1081 city: AMSTERDAM postcode: 1081HV website: n.a. contact info title: n.a. name: n.a. surname: n.a. function: n.a. email: n.a. telephone: n.a. fax: n.a.	NL (AMSTERDAM)	participant	937˙500.00
3	KOBENHAVNS UNIVERSITET KOBENHAVNS UNIVERSITET Organization address address: NORREGADE 10 city: KOBENHAVN postcode: 1165 website: www.ku.dk contact info title: n.a. name: n.a. surname: n.a. function: n.a. email: n.a. telephone: n.a. fax: n.a.	DK (KOBENHAVN)	participant	747˙320.00
4	DANMARKS TEKNISKE UNIVERSITET DANMARKS TEKNISKE UNIVERSITET Organization address address: ANKER ENGELUNDSVEJ 1 BYGNING 101 A city: KGS LYNGBY postcode: 2800 website: www.dtu.dk contact info title: n.a. name: n.a. surname: n.a. function: n.a. email: n.a. telephone: n.a. fax: n.a.	DK (KGS LYNGBY)	participant	716˙468.00
5	UNIVERSITY COLLEGE LONDON UNIVERSITY COLLEGE LONDON Organization address address: GOWER STREET city: LONDON postcode: WC1E 6BT website: n.a. contact info title: n.a. name: n.a. surname: n.a. function: n.a. email: n.a. telephone: n.a. fax: n.a.	UK (LONDON)	participant	111˙732.00

Map

Project objective

CHROMAVISION aims to develop a pioneering chromosome imaging and manipulation platform that will fuel the next decades of structural chromosome research. Chromosomal abnormalities are characteristic of many disorders such as cancer, impaired fertility due to maternal aging, and neurological disorders such as fragile X syndrome. If humanity is to fully understand the wide range of diseases that are associated to errors in cell division, we must be able to further 'zoom in' on healthy and diseased chromosomes in all their complexity. The CHROMAVISION platform will allow molecular biologists to automatically isolate individual chromosomes from small tissue or cell samples and have these delivered to a super-resolution microscope. Chromosome isolation and delivery is achieved by an opto-fluidic chip that is able to trap, visualise and lyse individual cells and separate metaphase chromosomes from cell lysate. Single chromosomes can be “hand-selected” and brought into focus of the Super-Resolution Correlative Tweezers Fluorescence Microscope (CTFM-SR3D) that is developed in CHROMAVISION. This instrument will for the first time enable 3D, super-resolution, real-time metaphase chromosome observation and manipulation studies under near-physiological conditions. The technique will push the boundaries of what is currently possible in microfluidics and super-resolution microscopy and combine these into a single powerful approach for chromosome studies. Furthermore, the platform will be applied in CHROMAVISION to address key challenges in clinical and fundamental chromosome research, potentially resulting in breakthrough discoveries. Better imaging and understanding of the chromosomal mechanisms will contribute to our knowledge of the etiology of human diseases and aid drug discovery. The platform will also have large clinical value, allowing identification and monitoring of e.g. cancer heterogeneity.

Deliverables

List of deliverables.
LOC for single cell capture and lysis	Demonstrators, pilots, prototypes	2019-11-12 11:27:48
Second 3DSR-CTFM instrument	Demonstrators, pilots, prototypes	2019-11-12 11:27:41
LoC prototypes for single cell chromosome analysis	Demonstrators, pilots, prototypes	2019-11-12 11:27:41
Super-resolutions movies of segragation	Websites, patent fillings, videos etc.	2019-11-12 11:27:29
Reproducible force-distance curves	Documents, reports	2019-11-12 11:27:28
LOC for single cell chromosome analysis	Demonstrators, pilots, prototypes	2019-11-12 11:27:17
Instrument and workflow for chromosome studies	Demonstrators, pilots, prototypes	2019-11-12 11:27:22
Validation CTFM-SR3D in biological/medical setting	Demonstrators, pilots, prototypes	2019-11-12 11:27:21
2 LOC to emulate chromatid segregation	Demonstrators, pilots, prototypes	2019-11-12 11:27:24
Real-time data of UFB formation and resolution	Documents, reports	2019-11-12 11:27:20
Field tested LOC for metaphase chromosome analysis	Demonstrators, pilots, prototypes	2019-11-12 11:27:17
Protocol for producing 3D super-resolution images	Documents, reports	2019-07-25 13:03:10
LOC for integration with optical tweezers & STED	Demonstrators, pilots, prototypes	2019-07-25 13:03:10
First working 3DSR-CTFM instrument	Demonstrators, pilots, prototypes	2019-07-25 13:03:10
Ethics documentation and Policies	Documents, reports	2019-07-25 13:03:10
Collaborative and public website	Websites, patent fillings, videos etc.	2019-07-25 13:03:10
Detailed communication and dissemination plan	Documents, reports	2019-07-25 13:03:10
Bead-handles	Demonstrators, pilots, prototypes	2019-07-25 13:03:10
Dissemination material & updates	Websites, patent fillings, videos etc.	2019-07-25 13:03:10

Take a look to the deliverables list in detail: detailed list of CHROMAVISION deliverables.

Publications

List of publications.
year	authors and title	journal	last update
2018	Rodolphe Marie, Jonas N. Pedersen, Kalim U. Mir, Brian Bilenberg, Anders Kristensen Concentrating and labeling genomic DNA in a nanofluidic array published pages: 1376-1382, ISSN: 2040-3364, DOI: 10.1039/c7nr06016e	Nanoscale 10/3	2019-08-05
2018	Kata SarlÃ³s, Andreas S. Biebricher, Anna H. Bizard, Julia A. M. Bakx, Anna G. FerretÃ©-Bonastre, Mauro Modesti, Manikandan Paramasivam, Qi Yao, Erwin J. G. Peterman, Gijs J. L. Wuite, Ian D. Hickson Reconstitution of anaphase DNA bridge recognition and disjunction published pages: 868-876, ISSN: 1545-9993, DOI: 10.1038/s41594-018-0123-8	Nature Structural & Molecular Biology 25/9	2019-08-05
2018	Victoria A. Bjerregaard, Lorenza Garribba, Cynthia T. McMurray, Ian D. Hickson, Ying Liu Folate deficiency drives mitotic missegregation of the human FRAXA locus published pages: 13003-13008, ISSN: 0027-8424, DOI: 10.1073/pnas.1808377115	Proceedings of the National Academy of Sciences 115/51	2019-08-05
2018	Marcus S. Carstensen, Xiaolong Zhu, Oseze Esther Iyore, N. Asger Mortensen, Uriel Levy, Anders Kristensen Holographic Resonant Laser Printing of Metasurfaces Using Plasmonic Template published pages: 1665-1670, ISSN: 2330-4022, DOI: 10.1021/acsphotonics.7b01358	ACS Photonics 5/5	2019-08-05
2018	Ã–zgÃ¼n Ã–zer, Rahul Bhowmick, Ying Liu, Ian D. Hickson Human cancer cells utilize mitotic DNA synthesis to resist replication stress at telomeres regardless of their telomere maintenance mechanism published pages: , ISSN: 1949-2553, DOI: 10.18632/oncotarget.24745	Oncotarget 9/22	2019-07-25
2018	Victoria A. Bjerregaard, Ã–zgÃ¼n Ã–zer, Ian D. Hickson, Ying Liu The detection and analysis of chromosome fragile sites published pages: 471-482, ISSN: , DOI: 10.1007/978-1-4939-7306-4_31	Methods in Molecular Biology	2019-07-25
2018	Johan Eriksen, Julien Schira, Nadine Vincent, Celine Sabatel, Anders Kristensen, Rodolphe Marie A lab-in-a-foil microfluidic reactor based on phaseguiding published pages: 14-20, ISSN: 0167-9317, DOI: 10.1016/j.mee.2017.11.011	Microelectronic Engineering 187-188	2019-07-25
2017	Rahul Bhowmick, Ian D Hickson \"The \"\"enemies within\"\": regions of the genome that are inherently difficult to replicate\" published pages: 666, ISSN: 2046-1402, DOI: 10.12688/f1000research.11024.1	F1000Research 6	2019-07-25
2017	Ganesha P. Pitchai, Manuel Kaulich, Anna H. Bizard, Pablo Mesa, Qi Yao, Kata Sarlos, Werner W. Streicher, Erich A. Nigg, Guillermo Montoya, Ian D. Hickson A novel TPRâ€“BEN domain interaction mediates PICHâ€“BEND3 association published pages: 11413-11424, ISSN: 0305-1048, DOI: 10.1093/nar/gkx792	Nucleic Acids Research 45/19	2019-07-25
2017	Johan Eriksen, Brian Bilenberg, Anders Kristensen, Rodolphe Marie Optothermally actuated capillary burst valve published pages: 45101, ISSN: 0034-6748, DOI: 10.1063/1.4979164	Review of Scientific Instruments 88/4	2019-07-25
2017	Xiaolong Zhu, Wei Yan, Uriel Levy, N. Asger Mortensen, Anders Kristensen Resonant laser printing of structural colors on high-index dielectric metasurfaces published pages: e1602487, ISSN: 2375-2548, DOI: 10.1126/sciadv.1602487	Science Advances 3/5	2019-07-25
2017	Cameron L. C. Smith, Anil H. Thilsted, Jonas N. Pedersen, Tomas H. Youngman, Julia C. Dyrnum, Nicolai A. Michaelsen, Rodolphe Marie, Anders Kristensen Photothermal Transport of DNA in Entropy-Landscape Plasmonic Waveguides published pages: 4553-4563, ISSN: 1936-0851, DOI: 10.1021/acsnano.6b08563	ACS Nano 11/5	2019-07-25
2017	Oren Lotan, Jonathan Bar-David, Cameron L.C. Smith, Sharon Yagur-Kroll, Shimshon Belkin, Anders Kristensen, Uriel Levy Nanoscale Plasmonic V-Groove Waveguides for the Interrogation of Single Fluorescent Bacterial Cells published pages: 5481-5488, ISSN: 1530-6984, DOI: 10.1021/acs.nanolett.7b02132	Nano Letters 17/9	2019-07-25
2017	Nicolai B. Larsen, Sascha E. Liberti, Ivan Vogel, Signe W. JÃ¸rgensen, Ian D. Hickson, Hocine W. Mankouri Stalled replication forks generate a distinct mutational signature in yeast published pages: 9665-9670, ISSN: 0027-8424, DOI: 10.1073/pnas.1706640114	Proceedings of the National Academy of Sciences 114/36	2019-07-25
2018	Kata SarlÃ³s, Andreas Biebricher, Erwin J.G. Petermann, Gijs J.L. Wuite, Ian D. Hickson Knotty Problems during Mitosis: Mechanistic Insight into the Processing of Ultrafine DNA Bridges in Anaphase published pages: 33647, ISSN: 0091-7451, DOI: 10.1101/sqb.2017.82.033647	Cold Spring Harbor Symposia on Quantitative Biology	2019-07-25
2017	Liqun Ren, Long Chen, Wei Wu, Lorenza Garribba, Huanna Tian, Zihui Liu, Ivan Vogel, Chunhui Li, Ian D. Hickson, Ying Liu Potential biomarkers of DNA replication stress in cancer published pages: , ISSN: 1949-2553, DOI: 10.18632/oncotarget.16940	Oncotarget	2019-07-25

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