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Opendata, web and dolomites

EvoMachine-Phage SIGNED

Using the Evolution Machine to determine the mechanism of a novel Phage-Induced High-Cell-Density (PIHCD) phenotype

Total Cost €

EC-Contrib. €

Partnership

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Outcomes and
results

EvoMachine-Phage project word cloud

Explore the words cloud of the EvoMachine-Phage project. It provides you a very rough idea of what is the project "EvoMachine-Phage" about.

skills termed evolution espci drive responsible genetic cycling understand poorly droplet wp2 bioinformatics bacteria paris intimately coinfection fluorescens biology fellowship transcriptomics pseudomonas phenotype basis density natural genomic machine manipulation cell antagonistic combined gain despite transfer microbiology material pihcd molecular carbon phages communities wp1 wp3 bacteriophages global expertise biological bacterial mechanistic maintenance device health hosts phage mechanism host human microfluidic coevolution

Project "EvoMachine-Phage" data sheet

The following table provides information about the project.

Coordinator	ECOLE SUPERIEURE DE PHYSIQUE ET DECHIMIE INDUSTRIELLES DE LA VILLE DEPARIS Organization address address: RUE VAUQUELIN 10 city: PARIS postcode: 75231 website: www.espci-paristech.fr contact info title: n.a. name: n.a. surname: n.a. function: n.a. email: n.a. telephone: n.a. fax: n.a.
Coordinator Country	France [FR]
Project website	http://www.stevenquistad.com/
Total cost	173˙076 €
EC max contribution	173˙076 € (100%)
Programme	1. H2020-EU.1.3.2. (Nurturing excellence by means of cross-border and cross-sector mobility)
Code Call	H2020-MSCA-IF-2016
Funding Scheme	MSCA-IF-EF-ST
Starting year	2017
Duration (year-month-day)	from 2017-04-01 to 2019-03-31

Partnership

Take a look of project's partnership.

#	participants	country	role	EC contrib. [€]
1	ECOLE SUPERIEURE DE PHYSIQUE ET DECHIMIE INDUSTRIELLES DE LA VILLE DEPARIS ECOLE SUPERIEURE DE PHYSIQUE ET DECHIMIE INDUSTRIELLES DE LA VILLE DEPARIS Organization address address: RUE VAUQUELIN 10 city: PARIS postcode: 75231 website: www.espci-paristech.fr contact info title: n.a. name: n.a. surname: n.a. function: n.a. email: n.a. telephone: n.a. fax: n.a.	FR (PARIS)	coordinator	173˙076.00

Map

Project objective

Bacteriophages (phages) drive the evolution of bacteria by antagonistic coevolution and by promoting transfer of genetic material between bacteria. Phages are intimately involved in a range of biological processes from carbon cycling to the maintenance of human health. Despite their global importance the coevolution between natural phage communities and their bacterial hosts remains poorly understood. This project seeks to understand the mechanism behind a Phage-Induced High-Cell-Density (PIHCD) phenotype using a droplet-based microfluidic device termed the Evolution Machine, developed at the proposed institution (ESPCI Paris). Work Package 1 (WP1) will use the Evolution Machine to determine and characterize the phage(s) responsible for the PIHCD phenotype through coinfection with their Pseudomonas fluorescens host. WP2 will determine the mechanistic basis of the PIHCD phenotype using transcriptomics and genetic manipulation. Finally, in WP3 the long term coevolution between phage(s) and host will be investigated using the droplet-level selection capabilities of the Evolution Machine combined with comparative genomic analysis. Through this fellowship ESPCI Paris will gain from my expertise in molecular biology and bioinformatics while I will develop new skills in microbiology (phage-host coevolution and genetic manipulation) and microfluidic technology (Evolution Machine).

Publications

List of publications.
year	authors and title	journal	last update
2019	Steven D. Quistad, Guilhem Doulcier, Paul B. Rainey Experimental manipulation of selfish genetic elements links genes to microbial community function published pages: , ISSN: , DOI: 10.1101/608752	BioRxiv	2019-05-15

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The information about "EVOMACHINE-PHAGE" are provided by the European Opendata Portal: CORDIS opendata.