DeE-Nano SIGNED
Design and Engineering Next-Generation Nanopore Devices for Bioplymer Analysis
Total Cost €
0EC-Contrib. €
0Partnership
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0DeE-Nano project word cloud
Explore the words cloud of the DeE-Nano project. It provides you a very rough idea of what is the project "DeE-Nano" about.
Project "DeE-Nano" data sheet
The following table provides information about the project.
| Coordinator |
RIJKSUNIVERSITEIT GRONINGEN
Organization address contact info |
| Coordinator Country | Netherlands [NL] |
| Total cost | 1˙999˙970 € |
| EC max contribution | 1˙999˙970 € (100%) |
| Programme |
1. H2020-EU.1.1. (EXCELLENT SCIENCE - European Research Council (ERC)) |
| Code Call | ERC-2016-COG |
| Funding Scheme | ERC-COG |
| Starting year | 2017 |
| Duration (year-month-day) | from 2017-07-01 to 2022-06-30 |
Partnership
Take a look of project's partnership.
| # | ||||
|---|---|---|---|---|
| 1 | RIJKSUNIVERSITEIT GRONINGEN | NL (GRONINGEN) | coordinator | 1˙999˙970.00 |
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Project objective
Nanopores have emerged in the past few years as a promising analytical technique. The basic concept of nanopore sensing is to apply a potential across individual nanoscale pores and observe the disruption of the ionic flow caused by single molecules entering the pore. Ionic currents through protein pores have been successful at recognizing tiny differences in molecules in solution. Most notably, arrays of thousands of nanopores integrated in low-cost and portable devices are now capable of sequencing DNA at the single-molecule level. The main challenge of nanopore sensing is the inability of controlling the protein pore diameter and geometry, which determines the signal and enables selectivity based on physical size.
The aim of this proposal is to design a new generation of protein nanopores that will take on the next grand challenge in nanopore sensing, that is the sequence identification of single proteins.
In order to sequence proteins, the designed nanopores must: unfold a target protein, control the speed of its transit across the nanopore and recognize individual amino acids. Our approach is to design a transmembrane molecular machine that will unfold target proteins and feed the linearize polypeptide through the nanopore where single amino acids will be recognized by modulations of the nanopore current. The specific objectives are: i) Develop chemical and biotechnological methods to design synthetic protein-based pores ii) To precisely attach the unfolding machine to a nanopore iii) To genetically engineer the nanopore for optimal amino acid recognition
Our nanopore devices will be used to develop the first technology to sequence single proteins. Compared to the state of the art ‘shotgun proteomics’, the nanopore approach will allow long polypeptide reads, recognition of low-abundance proteins, including biomarkers linked to diseases, and real-time monitoring with minimal cost, time and sample preparation.
Publications
| year | authors and title | journal | last update |
|---|---|---|---|
| 2019 |
Laura Restrepo-Pérez, Chun Heung Wong, Giovanni Maglia, Cees Dekker, Chirlmin Joo Label-Free Detection of Post-translational Modifications with a Nanopore published pages: 7957-7964, ISSN: 1530-6984, DOI: 10.1021/acs.nanolett.9b03134 |
Nano Letters 19/11 | 2020-04-01 |
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