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OMPorg SIGNED

Spatiotemporal organisation of bacterial outer membrane proteins

Total Cost €

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EC-Contrib. €

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Partnership

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 OMPorg project word cloud

Explore the words cloud of the OMPorg project. It provides you a very rough idea of what is the project "OMPorg" about.

poorly    poles    ensemble    imp    interrelated    spatiotemporal    omp    negative    purified    imps    antibiotics    inner    coli    outer    proteomics    biomedicine    vivo    grow    disrupt    connected    functions    route    connect    populations    protects    island    hence    unknown    live    species    partitioning    processes    bridges    mimicked    proteins    laboratory    molecular    single    islands    coordinate    molecule    repository    omporg    bacterial    integrity    binary    cells    influence    om    protein    escherichia    questions    central    cell    limited    basis    formation    outstanding    answer    environmental    underpin    tools    turned    septation    simulations    biotechnology    endow    bilayers    discovered    memory    mediate    gram    periplasm    bacteria    omps    biology    interdisciplinary    aeruginosa    becomes    envelope    imposed    pathogenesis    ramifications    displaced    organising    membrane    ask    cluster    clustering    incorporating    concerning    caused    found    organisation    insult    microscopy    explains    ways    chemistry    largely    impacts    im    immobility    dynamics   

Project "OMPorg" data sheet

The following table provides information about the project.

Coordinator
THE CHANCELLOR, MASTERS AND SCHOLARS OF THE UNIVERSITY OF OXFORD 

Organization address
address: WELLINGTON SQUARE UNIVERSITY OFFICES
city: OXFORD
postcode: OX1 2JD
website: www.ox.ac.uk

contact info
title: n.a.
name: n.a.
surname: n.a.
function: n.a.
email: n.a.
telephone: n.a.
fax: n.a.

 Coordinator Country United Kingdom [UK]
 Total cost 2˙235˙815 €
 EC max contribution 2˙235˙815 € (100%)
 Programme 1. H2020-EU.1.1. (EXCELLENT SCIENCE - European Research Council (ERC))
 Code Call ERC-2016-ADG
 Funding Scheme ERC-ADG
 Starting year 2017
 Duration (year-month-day) from 2017-09-01   to  2022-08-31

 Partnership

Take a look of project's partnership.

# participants  country  role  EC contrib. [€] 
1    THE CHANCELLOR, MASTERS AND SCHOLARS OF THE UNIVERSITY OF OXFORD UK (OXFORD) coordinator 2˙235˙815.00

Map

 Project objective

The outer membrane (OM) of Gram-negative bacteria protects against environmental insult and is central to pathogenesis hence finding ways to disrupt its integrity is a route towards new antibiotics. However, our understanding of OM biology is limited. In particular, how the OM is organised is largely unknown. My laboratory recently discovered spatiotemporal Outer Membrane Protein organisation (OMPorg) in Escherichia coli, a new organising principle that explains how OMPs are turned over. We found that OMPs cluster into islands that can be mimicked in supported bilayers using purified proteins. As cells grow, OMP islands are displaced to the poles by new islands, leading to binary partitioning of old OMPs in repository cells following septation.

Another poorly understood aspect of OM biology is how protein bridges that connect the OM to the inner membrane (IM) mediate functions across the periplasm. We have discovered that the characteristic immobility of OMPs in vivo, caused by OMP clustering, becomes imposed on IM proteins (IMPs) when OMPs and IMPs become connected by protein bridges.

These exciting new findings underpin OMPorg. I will focus on two species, E. coli and P. aeruginosa, and exploit the tools I have developed to ask four interrelated questions:

1. What is the molecular basis of OMP island formation? 2. Do OMPs influence IMP functionality via protein bridges? 3. Do repository cells endow bacterial populations with ‘OMP memory’? 4. Do OMP islands coordinate OM processes?

I will address these questions through an interdisciplinary research programme incorporating protein chemistry and proteomics, ensemble and single molecule microscopy on live cells and in supported bilayers and molecular dynamics simulations. OMPorg will answer major outstanding questions concerning organisation in the bacterial OM and how this impacts the biology of the cell envelope, which will have ramifications for biomedicine and biotechnology.

 Publications

year authors and title journal last update
List of publications.
2018 Patrice Rassam, Kathleen R. Long, Renata Kaminska, David J. Williams, Grigorios Papadakos, Christoph G. Baumann, Colin Kleanthous
Intermembrane crosstalk drives inner-membrane protein organization in Escherichia coli
published pages: , ISSN: 2041-1723, DOI: 10.1038/s41467-018-03521-4
Nature Communications 9/1 2019-04-18
2018 Matthieu Chavent, Anna L. Duncan, Patrice Rassam, Oliver Birkholz, Jean Hélie, Tyler Reddy, Dmitry Beliaev, Ben Hambly, Jacob Piehler, Colin Kleanthous, Mark S. P. Sansom
How nanoscale protein interactions determine the mesoscale dynamic organisation of bacterial outer membrane proteins
published pages: , ISSN: 2041-1723, DOI: 10.1038/s41467-018-05255-9
Nature Communications 9/1 2019-04-18
2019 Connor Sharp, Christine Boinett, Amy Cain, Nicholas G. Housden, Sandip Kumar, Keith Turner, Julian Parkhill, Colin Kleanthous
O-Antigen-Dependent Colicin Insensitivity of Uropathogenic Escherichia coli
published pages: , ISSN: 0021-9193, DOI: 10.1128/jb.00545-18
Journal of Bacteriology 201/4 2019-04-16

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