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EpiTrack SIGNED

Single-cell temporal tracking of epigenetic DNA marks

Total Cost €

EC-Contrib. €

Partnership

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EpiTrack project word cloud

Explore the words cloud of the EpiTrack project. It provides you a very rough idea of what is the project "EpiTrack" about.

methyl pulse mammals modifications dynamics over analog modification metabolically questions cell dramatic methylation sam whereby time fertilization massive enzymatic give human view events dna precise variability single barcoding methyltransferases gap resolved neural experimental phenomena mechanistic deposited marks fascinating roles groups transfer reprogramming bridge occurs adenosylmethionine decade dnmts engineer individual microdroplet expression lineages innovative cellular imaging play integration epigenetic reinstatement patterns global govern genomic epigenomic profiling tagging horizons mapping regulation chemical followed extensively unprecedented embryonic obscure mammalian prevalent answered disease mouse permit stage throughput plasticity cells resolution differentiation super vivo sites cofactor centre gene

Project "EpiTrack" data sheet

The following table provides information about the project.

Coordinator	VILNIAUS UNIVERSITETAS Organization address address: UNIVERSITETO G. 3 city: VILNIUS postcode: 1513 website: http://www.vu.lt contact info title: n.a. name: n.a. surname: n.a. function: n.a. email: n.a. telephone: n.a. fax: n.a.
Coordinator Country	Lithuania [LT]
Total cost	2˙499˙875 €
EC max contribution	2˙499˙875 € (100%)
Programme	1. H2020-EU.1.1. (EXCELLENT SCIENCE - European Research Council (ERC))
Code Call	ERC-2016-ADG
Funding Scheme	ERC-ADG
Starting year	2017
Duration (year-month-day)	from 2017-09-01 to 2022-08-31

Partnership

Take a look of project's partnership.

#	participants	country	role	EC contrib. [€]
1	VILNIAUS UNIVERSITETAS VILNIAUS UNIVERSITETAS Organization address address: UNIVERSITETO G. 3 city: VILNIUS postcode: 1513 website: http://www.vu.lt contact info title: n.a. name: n.a. surname: n.a. function: n.a. email: n.a. telephone: n.a. fax: n.a.	LT (VILNIUS)	coordinator	2˙499˙875.00

Map

Project objective

Over the past decade, epigenetic phenomena have taken centre stage in our understanding of gene regulation, cellular differentiation and human disease. DNA methylation is a prevalent epigenetic modification in mammals, which is brought about by enzymatic transfer of methyl groups from the S-adenosylmethionine (SAM) cofactor by three known DNA methyltransferases (DNMTs). The most dramatic epigenomic reprogramming in mammalian development occurs after fertilization, whereby a global loss of DNA methylation is followed by massive reinstatement of new methylation patterns, different for each cell type. Although DNA methylation has been extensively investigated, key mechanistic aspects of these fascinating events remain obscure. The goal of this proposal is to bridge the gap in our understanding of how the genomic methylation patterns are established and how they govern cell plasticity and variability during differentiation and development. These questions could only be answered by precise determination of where and when methylation marks are deposited by the individual DNMTs, and how these methylation marks affect gene expression. To achieve this ambitious goal, we will metabolically engineer mouse cells to permit SAM analog-based chemical pulse-tagging of their methylation sites in vivo. We will then advance profiling of DNA modifications to the single cell level via innovative integration of microdroplet-based barcoding, precise genomic mapping and super-resolution imaging. Using this unique experimental system we will determine, with unprecedented detail and throughput, the dynamics and variability of DNA methylation and gene expression patterns during differentiation of mouse embryonic cells to neural and other lineages. This project will give a comprehensive, time-resolved view of the roles that the DNMTs play in mammalian development, which will open new horizons in epigenomic research and will advance our understanding of human development and disease.

Publications

List of publications.
year	authors and title	journal	last update
2020	Povilas Gibas, Milda NarmontÄ—, Zdislav StaÅ¡evskij, Juozas GordeviÄius, Saulius KlimaÅ¡auskas, Edita KriukienÄ— Precise genomic mapping of 5-hydroxymethylcytosine via covalent tether-directed sequencing published pages: e3000684, ISSN: 1544-9173, DOI: 10.1371/journal.pbio.3000684	PLOS Biology 18	2020-04-15
2018	Milda MickutÄ—, Milda NainytÄ—, Lina VasiliauskaitÄ—, Alexandra Plotnikova, Viktoras MaseviÄius, Saulius KlimaÅ¡auskas, Giedrius Vilkaitis Animal Hen1 2â€²-O-methyltransferases as tools for 3â€²-terminal functionalization and labelling of single-stranded RNAs published pages: e104-e104, ISSN: 0305-1048, DOI: 10.1093/nar/gky514	Nucleic Acids Research 46/17	2019-04-18
2019	Saulius KlimaÅ¡auskas, Linas MaÅ¾utis Editorial overview: Current advances in analytical biotechnology: from single molecules to whole organisms published pages: , ISSN: 0958-1669, DOI: 10.1016/j.copbio.2018.12.006	Current Opinion in Biotechnology	2019-02-28
2019	MiglÄ— TomkuvienÄ—, Milda MickutÄ—, Giedrius Vilkaitis, Saulius KlimaÅ¡auskas Repurposing enzymatic transferase reactions for targeted labeling and analysis of DNA and RNA published pages: 114-123, ISSN: 0958-1669, DOI: 10.1016/j.copbio.2018.09.008	Current Opinion in Biotechnology 55	2019-02-12

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The information about "EPITRACK" are provided by the European Opendata Portal: CORDIS opendata.