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MASTFAST

Rapid production of HUMAN MAST CELLS

Total Cost €

EC-Contrib. €

Partnership

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MASTFAST project word cloud

Explore the words cloud of the MASTFAST project. It provides you a very rough idea of what is the project "MASTFAST" about.

made translate crispr 12 hampered damage interact connective cas9 dysregulation mutant functional allergies instead calls enrichment time intestinal normal cells gene validate embryonic patient chronic bone too alternative strategies anaphylaxsis yields limit pain mastocytoma granules ground population produces medicine breaking complement function pathogen inability 341096 few mast ige marrow differentiation esc tissue phenotypic reporter human rapid components healing release adg hescs goals mouse wound industry erc engineered molecules stem optimize allergic autism weeks damaging unexpected dysmotility escs mucosal fatigue activated treatments proteases treatment drug discovery hematopoietic homogenous implicated airways hesc cultures grow personalized angiogenesis skin syndrome culture cell dysfunction

Project "MASTFAST" data sheet

The following table provides information about the project.

Coordinator	THE UNIVERSITY OF EDINBURGH Organization address address: OLD COLLEGE, SOUTH BRIDGE city: EDINBURGH postcode: EH8 9YL website: www.ed.ac.uk contact info title: n.a. name: n.a. surname: n.a. function: n.a. email: n.a. telephone: n.a. fax: n.a.
Coordinator Country	United Kingdom [UK]
Total cost	148˙914 €
EC max contribution	148˙914 € (100%)
Programme	1. H2020-EU.1.1. (EXCELLENT SCIENCE - European Research Council (ERC))
Code Call	ERC-2017-PoC
Funding Scheme	ERC-POC
Starting year	2017
Duration (year-month-day)	from 2017-12-01 to 2019-05-31

Partnership

Take a look of project's partnership.

#	participants	country	role	EC contrib. [€]
1	THE UNIVERSITY OF EDINBURGH THE UNIVERSITY OF EDINBURGH Organization address address: OLD COLLEGE, SOUTH BRIDGE city: EDINBURGH postcode: EH8 9YL website: www.ed.ac.uk contact info title: n.a. name: n.a. surname: n.a. function: n.a. email: n.a. telephone: n.a. fax: n.a.	UK (EDINBURGH)	coordinator	148˙914.00

Map

Project objective

Mast cells are involved in the allergic response, anaphylaxsis, wound healing and angiogenesis. When activated via IgE, damage/pathogen-induced molecules or complement components, the granules of mast cells release proteases. Dysregulation of mast cells is implicated in allergies of the skin and airways, autism, chronic fatigue syndrome, pain, mastocytoma and intestinal dysmotility. Strategies to limit the damaging effects of mast calls are needed. However, the development of novel treatments is hampered by the inability to grow mast cells rapidly and efficiently in culture. Bone marrow cell cultures typically take 12 weeks before mast cells are available for study. Even then, there are too few cells to use for drug discovery or patient-specific treatment strategies. Embryonic stem cells (ESC) represent an alternative method for the production of mast cells. An unexpected ground-breaking discovery from our ERC AdG 341096 studies aiming to produce hematopoietic stem cells, was the development of a novel method that instead produces large numbers of mast cells in a short time. Mouse ESC engineered with a unique reporter gene that allows for cell enrichment during a multi-step culture yields a homogenous population of phenotypic and functional connective tissue and mucosal mast cells. Within only 3 weeks large numbers of mast cells are generated. To translate this method for large scale rapid production of human mast cells we will 1) characterize unique human reporter ESCs made by Crispr/CAS9 state-of-the-art method; 2) optimize human mast cell production from reporter hESCs in a multi-step differentiation culture; 3) characterize/validate the function of hESC-derived mast cells (normal and mutant); 4) interact with industry to use hESC-derived mast cells for drug-discovery and studies of mast cell differentiation and dysfunction. Long-term goals include the development of mast cell treatment strategies for personalized medicine.

Publications

List of publications.
year	authors and title	journal	last update
2018	Mari-Liis Kauts, Bianca De Leo, Carmen RodrÃguez-Seoane, Roger Ronn, Fokion Glykofrydis, Antonio Maglitto, Polynikis Kaimakis, Margarita Basi, Helen Taylor, Lesley Forrester, Adam C. Wilkinson, Berthold GÃ¶ttgens, Philippa Saunders, Elaine Dzierzak Rapid Mast Cell Generation from Gata2 Reporter Pluripotent Stem Cells published pages: 1009-1020, ISSN: 2213-6711, DOI: 10.1016/j.stemcr.2018.08.007	Stem Cell Reports 11/4	2020-01-23

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