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Electrochemically Switchable Fluorescent Probes for Biological Applications

Total Cost €


EC-Contrib. €






Project "ELSWIFLUOPRO" data sheet

The following table provides information about the project.


Organization address
address: 45, RUE D'ULM
city: PARIS CEDEX 05
postcode: 75230

contact info
title: n.a.
name: n.a.
surname: n.a.
function: n.a.
email: n.a.
telephone: n.a.
fax: n.a.

 Coordinator Country France [FR]
 Project website
 Total cost 185˙076 €
 EC max contribution 185˙076 € (100%)
 Programme 1. H2020-EU.1.3.2. (Nurturing excellence by means of cross-border and cross-sector mobility)
 Code Call H2020-MSCA-IF-2014
 Funding Scheme MSCA-IF-EF-ST
 Starting year 2015
 Duration (year-month-day) from 2015-09-01   to  2017-08-31


Take a look of project's partnership.

# participants  country  role  EC contrib. [€] 
1    ECOLE NORMALE SUPERIEURE FR (PARIS CEDEX 05) coordinator 185˙076.00


 Project objective

In current drug development, the visualization of therapeutic-agent translocation to the cell interior represents one of the essential problems. The transport across cellular membranes is frequently executed by cell-penetrating peptides (CPPs), and the process is routinely studied with fluorescence microscopy when the CPP is attached to a fluorescent marker. Nevertheless, the use of existing labelled CPPs is connected with several drawbacks. Especially, bonding of CPPs to the external face of the cell membrane make interpretation of the translocation difficult and can result in false positive results. Therefore, the development of novel electrochemically switchable fluorescent probe for the detection of CPP translocation is suggested. In this project, a fluorescent organic dye will be coupled with an electro-controlled fluorescent quencher, which could be activated/deactivated in response to applied electric potential. As the electron transfer cannot occur through the cell membrane, labelled external CPPs would be switched off through photoinduced electron transfer, and fluorescence only of internalized CPPs could be studied. Reversibility of the system represents a remarkable advantage as it would allow repetitive on/off switching of the fluorescent probe.


year authors and title journal last update
List of publications.
2018 Čížková, M.; Cattiaux, L.; Mallet, J.-M.; Labbé, E.; Buriez, O.
Redox-controlled fluorescence modulation in NBD-Ferrocene derivative (in preparation)
published pages: , ISSN: 0013-4686, DOI:
Electrochimica Acta 2019-09-02
2018 Martina Čížková, Laurent Cattiaux, Jean-Maurice Mallet, Eric Labbé, Olivier Buriez
Electrochemical switching fluorescence emission in rhodamine derivatives
published pages: 589-597, ISSN: 0013-4686, DOI: 10.1016/j.electacta.2017.12.104
Electrochimica Acta 260 2019-09-02
2018 Martina Čížková, Laurent Cattiaux, Justine Pandard, Manon Guille-Collignon, Frédéric Lemaître, Jérôme Delacotte, Jean-Maurice Mallet, Eric Labbé, Olivier Buriez
Redox switchable rhodamine-ferrocene dyad: Exploring imaging possibilities in cells
published pages: 46-50, ISSN: 1388-2481, DOI: 10.1016/j.elecom.2018.10.009
Electrochemistry Communications 97 2019-09-02

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