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AdLibYeast SIGNED

Synthetic platforms for ad libitum remodelling of yeast central metabolism

Total Cost €

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EC-Contrib. €

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Partnership

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 AdLibYeast project word cloud

Explore the words cloud of the AdLibYeast project. It provides you a very rough idea of what is the project "AdLibYeast" about.

experimenters    breaking    innovative    remodel    hurdle    restricts    engineering    industrial    microbes    performance    metabolism    irrespective    modular    specialized    endless    carbon    depends    mosaic    relocated    conquer    central    cerevisiae    biotechnology    genes    chromosomes    machinery    networks    petrochemistry    easily    construct    core    ground    optimize    regulation    native    genomes    swapping    horse    scattered    swapped    efficiency    organization    cell    structure    variety    metabolic    sustainable    introduce    aeons    absence    genome    function    evolution    questions    model    configurations    equipped    chromosome    fast    unanswered    encoded    heterologous    biotechnological    constraints    homologous    shaped    paves    optimized    offers    synthetic    fitness    principles    hitherto    optimization    eukaryotic    fundamental    chassis    saccharomyces    accessibility    nature    purposes    replacement    unifying    ultimate    rational    combinatorial    possibilities    engineered    bio    genetic    enzymes    yeast    limitation    sheer    encoding    microbial    tremendously   

Project "AdLibYeast" data sheet

The following table provides information about the project.

Coordinator
TECHNISCHE UNIVERSITEIT DELFT 

Organization address
address: STEVINWEG 1
city: DELFT
postcode: 2628 CN
website: www.tudelft.nl

contact info
title: n.a.
name: n.a.
surname: n.a.
function: n.a.
email: n.a.
telephone: n.a.
fax: n.a.

 Coordinator Country Netherlands [NL]
 Total cost 2˙149˙717 €
 EC max contribution 2˙149˙717 € (100%)
 Programme 1. H2020-EU.1.1. (EXCELLENT SCIENCE - European Research Council (ERC))
 Code Call ERC-2014-CoG
 Funding Scheme ERC-COG
 Starting year 2015
 Duration (year-month-day) from 2015-09-01   to  2020-08-31

 Partnership

Take a look of project's partnership.

# participants  country  role  EC contrib. [€] 
1    TECHNISCHE UNIVERSITEIT DELFT NL (DELFT) coordinator 2˙149˙717.00

Map

 Project objective

Replacement of petrochemistry by bio-based processes is key to sustainable development and requires microbes equipped with novel-to-nature capabilities. The efficiency of such engineered microbes strongly depends on their native metabolic networks. However, aeons of evolution have optimized these networks for fitness in nature rather than for industrial performance. As a result, central metabolic networks are complex and encoded by mosaic microbial genomes in which genes, irrespective of their function, are scattered over the genome and chromosomes. This absence of a modular organization tremendously restricts genetic accessibility and presents a major hurdle for fundamental understanding and rational engineering of central metabolism. To conquer this limitation, I introduce the concept of ‘pathway swapping’, which will enable experimenters to remodel the core machinery of microbes at will. Using the yeast Saccharomyces cerevisiae, an industrial biotechnology work horse and model eukaryotic cell, I propose to design and construct a microbial chassis in which all genes encoding enzymes in central carbon metabolism are relocated to a specialized synthetic chromosome, from which they can be easily swapped by any – homologous or heterologous – synthetic pathway. This challenging and innovative project paves the way for a modular approach to engineering of central metabolism. Beyond providing a ground-breaking enabling technology, the ultimate goal of the pathway swapping technology is to address hitherto unanswered fundamental questions. Access to a sheer endless variety of configurations of central metabolism offers unique, new possibilities to study the fundamental design of metabolic pathways, the constraints that have shaped them and unifying principles for their structure and regulation. Moreover, this technology enables fast, combinatorial optimization studies on central metabolism to optimize its performance in biotechnological purposes.

 Publications

year authors and title journal last update
List of publications.
2018 Gemma Perez-Samper, Bram Cerulus, Abbas Jariani, Lieselotte Vermeersch, Nuria Barrajón Simancas, Markus M. M. Bisschops, Joost van den Brink, Daniel Solis-Escalante, Brigida Gallone, Dries De Maeyer, Elise van Bael, Tom Wenseleers, Jan Michiels, Kathleen Marchal, Pascale Daran-Lapujade, Kevin J. Verstrepen
The Crabtree Effect Shapes the Saccharomyces cerevisiae Lag Phase during the Switch between Different Carbon Sources
published pages: , ISSN: 2150-7511, DOI: 10.1128/mBio.01331-18
mBio 9/5 2019-04-18
2017 Michal A. Świat, Sofia Dashko, Maxime den Ridder, Melanie Wijsman, John van der Oost, Jean-Marc Daran, Pascale Daran-Lapujade
FnCpf1: a novel and efficient genome editing tool for Saccharomyces cerevisiae
published pages: 12585-12598, ISSN: 0305-1048, DOI: 10.1093/nar/gkx1007
Nucleic Acids Research 45/21 2019-04-18
2018 Melanie Wijsman, Michał A Świat, Wesley L Marques, Johanna K Hettinga, Marcel van den Broek, Pilar de la Torre Cortés, Robert Mans, Jack T Pronk, Jean-Marc Daran, Pascale Daran-Lapujade
A toolkit for rapid CRISPR-SpCas9 assisted construction of hexose-transport-deficient Saccharomyces cerevisiae strains
published pages: , ISSN: 1567-1364, DOI: 10.1093/femsyr/foy107
FEMS Yeast Research 2019-04-18
2018 Francine J. Boonekamp, Sofia Dashko, Marcel van den Broek, Thies Gehrmann, Jean-Marc Daran, Pascale Daran-Lapujade
The Genetic Makeup and Expression of the Glycolytic and Fermentative Pathways Are Highly Conserved Within the Saccharomyces Genus
published pages: , ISSN: 1664-8021, DOI: 10.3389/fgene.2018.00504
Frontiers in Genetics 9 2019-04-18

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