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SegregActin SIGNED

Building Distinct Actin Filament Networks in a Common Cytoplasm

Total Cost €

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EC-Contrib. €

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Partnership

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Project "SegregActin" data sheet

The following table provides information about the project.

Coordinator
CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE CNRS 

Organization address
address: RUE MICHEL ANGE 3
city: PARIS
postcode: 75794
website: www.cnrs.fr

contact info
title: n.a.
name: n.a.
surname: n.a.
function: n.a.
email: n.a.
telephone: n.a.
fax: n.a.

 Coordinator Country France [FR]
 Total cost 1˙500˙000 €
 EC max contribution 1˙500˙000 € (100%)
 Programme 1. H2020-EU.1.1. (EXCELLENT SCIENCE - European Research Council (ERC))
 Code Call ERC-2014-STG
 Funding Scheme ERC-STG
 Starting year 2015
 Duration (year-month-day) from 2015-09-01   to  2021-08-31

 Partnership

Take a look of project's partnership.

# participants  country  role  EC contrib. [€] 
1    CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE CNRS FR (PARIS) coordinator 1˙500˙000.00
2    COMMISSARIAT A L ENERGIE ATOMIQUE ET AUX ENERGIES ALTERNATIVES FR (PARIS 15) participant 0.00

Map

 Project objective

'The ability of cells to use the actin cytoskeleton for a diversity of cellular processes is due to the fact that actin filaments, although assembled from identical subunits, are organized in a wide variety of structures of appropriate geometrical, dynamical and rheological properties. Key players in this regulation are specific sets of actin binding proteins (ABPs) interacting with each actin networks, to modulate spatially and temporally their properties. With this project, I want to understand 1/ how cells can generate the formation of actin structures of appropriate ABP composition from a common pool of cytoplasmic components and 2/ the relationship between the ABP composition of an actin network, its geometrical and dynamical properties, and its response to mechanical deformations.

I will hypothesize that the generation of an actin network of appropriate ABP composition can be explained with an original model, taking into account the facts that 1/ actin filaments in cells are not all structurally identical, but adopt specific conformations that are favored and stabilized by certain families of ABPs; and 2/ the interaction of ABPs with actin depends of the geometrical organization of the filaments.

Because this project imposes to study protein-protein interactions in the presence of multiple partners, I propose to develop an unprecedented strategy combining 1/ 'bottom-up' reconstitutions, where limited sets of ABPs are added one-by-one in the system to understand their combined activities with actin; and 2/ 'top-down' reconstitutions with protein extracts prepared from a genetically-tractable organism (the yeast S. cerevisiae), where proteins can be removed one-by-one, in order to study actin network properties in near-physiological conditions.

This project will shed a new light on how cells organize their interior, and will represent a unique opportunity to understand how modifications in the expression of ABPs are associated with actin network defects. '

 Publications

year authors and title journal last update
List of publications.
2017 Gergana Gateva, Elena Kremneva, Theresia Reindl, Tommi Kotila, Konstantin Kogan, Laurène Gressin, Peter W. Gunning, Dietmar J. Manstein, Alphée Michelot, Pekka Lappalainen
Tropomyosin Isoforms Specify Functionally Distinct Actin Filament Populations In Vitro
published pages: 705-713, ISSN: 0960-9822, DOI: 10.1016/j.cub.2017.01.018
Current Biology 27/5 2020-02-12
2019 Adrien Antkowiak, Audrey Guillotin, Micaela Boiero Sanders, Jessica Colombo, Renaud Vincentelli, Alphée Michelot
Sizes of actin networks sharing a common environment are determined by the relative rates of assembly
published pages: e3000317, ISSN: 1545-7885, DOI: 10.1371/journal.pbio.3000317
PLOS Biology 17/6 2020-02-12
2019 Jessica Planade, Reda Belbahri, Micaela Boiero Sanders, Audrey Guillotin, Olivia du Roure, Alphée Michelot, Julien Heuvingh
Mechanical stiffness of reconstituted actin patches correlates tightly with endocytosis efficiency
published pages: e3000500, ISSN: 1545-7885, DOI: 10.1371/journal.pbio.3000500
PLOS Biology 17/10 2020-02-12

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The information about "SEGREGACTIN" are provided by the European Opendata Portal: CORDIS opendata.

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