Opendata, web and dolomites

BENDER SIGNED

BiogENesis and Degradation of Endoplasmic Reticulum proteins

Total Cost €

0

EC-Contrib. €

0

Partnership

0

Views

0

 BENDER project word cloud

Explore the words cloud of the BENDER project. It provides you a very rough idea of what is the project "BENDER" about.

residing    cotranslational    depletion    maximum    im    cet    structure    regulation    translocon    network    ing    gateway    aging    biogene    translation    evasion    chaperone    proteins    sis    insights    tomograms    reveal    homeostasis    class    complexity    details    proteasome    stabilization    misfolded    transferase    maturation    mediated    cellular    genesis    immune    purification    membrane    cell    oligosaccharyl    surrounding    import    cytomegalovirus    eukaryotic    disorders    3d    chronic    macromolecule    sophisticated    pioneered    intricate    bio    protein    diseases    glycosylation    leverage    resolution    favours    subtomogram    nascent    cells    cryo    native    cytosolic    facilitates    26s    blueprint    neurodegenerative    structural    microenvironment    silico    folding    histocompatibility    conformations    reticulum    stress    sub    degradation    machinery    molecular    tomography    mechanistic    diabetes    computational    viral    ire1    biology    complexes    macromolecules    dynamic    depicting    substrate    mhc    endoplasmic    free    mrna    er    classification    electron    subnanometer    oligomerization    provides    model    bound   

Project "BENDER" data sheet

The following table provides information about the project.

Coordinator
UNIVERSITEIT UTRECHT 

Organization address
address: HEIDELBERGLAAN 8
city: UTRECHT
postcode: 3584 CS
website: www.uu.nl

contact info
title: n.a.
name: n.a.
surname: n.a.
function: n.a.
email: n.a.
telephone: n.a.
fax: n.a.

 Coordinator Country Netherlands [NL]
 Total cost 2˙496˙611 €
 EC max contribution 2˙496˙611 € (100%)
 Programme 1. H2020-EU.1.1. (EXCELLENT SCIENCE - European Research Council (ERC))
 Code Call ERC-2016-COG
 Funding Scheme ERC-COG
 Starting year 2017
 Duration (year-month-day) from 2017-04-01   to  2022-03-31

 Partnership

Take a look of project's partnership.

# participants  country  role  EC contrib. [€] 
1    UNIVERSITEIT UTRECHT NL (UTRECHT) coordinator 2˙496˙611.00

Map

 Project objective

The Endoplasmic Reticulum (ER) membrane in all eukaryotic cells has an intricate protein network that facilitates protein biogene-sis and homeostasis. The molecular complexity and sophisticated regulation of this machinery favours study-ing it in its native microenvironment by novel approaches. Cryo-electron tomography (CET) allows 3D im-aging of membrane-associated complexes in their native surrounding. Computational analysis of many sub-tomograms depicting the same type of macromolecule, a technology I pioneered, provides subnanometer resolution insights into different conformations of native complexes. I propose to leverage CET of cellular and cell-free systems to reveal the molecular details of ER protein bio-genesis and homeostasis. In detail, I will study: (a) The structure of the ER translocon, the dynamic gateway for import of nascent proteins into the ER and their maturation. The largest component is the oligosaccharyl transferase complex. (b) Cotranslational ER import, N-glycosylation, chaperone-mediated stabilization and folding as well as oligomerization of established model substrate such a major histocompatibility complex (MHC) class I and II complexes. (c) The degradation of misfolded ER-residing proteins by the cytosolic 26S proteasome using cytomegalovirus-induced depletion of MHC class I as a model system. (d) The structural changes of the ER-bound translation machinery upon ER stress through IRE1-mediated degradation of mRNA that is specific for ER-targeted proteins. (e) The improved ‘in silico purification’ of different states of native macromolecules by maximum likelihood subtomogram classification and its application to a-d. This project will be the blueprint for a new approach to structural biology of membrane-associated processes. It will contribute to our mechanistic understanding of viral immune evasion and glycosylation disorders as well as numerous diseases involving chronic ER stress including diabetes and neurodegenerative diseases.

 Publications

year authors and title journal last update
List of publications.
2018 Katharina Braunger, Stefan Pfeffer, Shiteshu Shrimal, Reid Gilmore, Otto Berninghausen, Elisabet C. Mandon, Thomas Becker, Friedrich Förster, Roland Beckmann
Structural basis for coupling protein transport and N-glycosylation at the mammalian endoplasmic reticulum
published pages: 215-219, ISSN: 0036-8075, DOI: 10.1126/science.aar7899
Science 360/6385 2019-06-11
2018 Patrique Praest, A. Manuel Liaci, Friedrich Förster, Emmanuel J.H.J. Wiertz
New insights into the structure of the MHC class I peptide-loading complex and mechanisms of TAP inhibition by viral immune evasion proteins
published pages: , ISSN: 0161-5890, DOI: 10.1016/j.molimm.2018.03.020
Molecular Immunology 2019-06-11

Are you the coordinator (or a participant) of this project? Plaese send me more information about the "BENDER" project.

For instance: the website url (it has not provided by EU-opendata yet), the logo, a more detailed description of the project (in plain text as a rtf file or a word file), some pictures (as picture files, not embedded into any word file), twitter account, linkedin page, etc.

Send me an  email (fabio@fabiodisconzi.com) and I put them in your project's page as son as possible.

Thanks. And then put a link of this page into your project's website.

The information about "BENDER" are provided by the European Opendata Portal: CORDIS opendata.

More projects from the same programme (H2020-EU.1.1.)

LO-KMOF (2019)

Vapour-deposited metal-organic frameworks as high-performance gap-filling dielectrics for nanoelectronics

Read More  

PLAT_ACE (2019)

A new platform technology for the on-demand access to large acenes

Read More  

TRUST (2018)

Truth and Semantics

Read More