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3D-Xist SIGNED

3D structure of the long non-coding RNA Xist by complementary cryo-electron tomography and single particle cryo-electron microscopy

Total Cost €

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EC-Contrib. €

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Partnership

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 3D-Xist project word cloud

Explore the words cloud of the 3D-Xist project. It provides you a very rough idea of what is the project "3D-Xist" about.

sub    cutting    particle    expressing    function    lncrna    excellent    single    atomic    composite    particles    communication    cancers    public    physiological    homogenous    disease    solving    facility    centre    rna    cryo    physiology    publishing    host    university    first    skills    resolution    brockdorff    structural    benefit    fantastic    biology    mes    generating    innovative    ing    world    pseudo    journals    female    electron    mechanism    pivotal    visualising    tomography    conferences    trainings    employ    visualise    career    structure    formulated    diseases    lncrnas    presenting    recent    ribonucleoprotein    techniques    normal    et    cell    xist    cellular    action    3d    scientist    preparing    engagement    coding    edge    infrastructure    supervisor    developmental    mammals    elegant    highlighted    lacking    molecular    prof    cells    overarching    date    oxford    em    rnp    rnas    integral    rnps    class    interdisciplinary    analysing    expanding    progeria    international    transferrable   

Project "3D-Xist" data sheet

The following table provides information about the project.

Coordinator
THE CHANCELLOR, MASTERS AND SCHOLARS OF THE UNIVERSITY OF OXFORD 

Organization address
address: WELLINGTON SQUARE UNIVERSITY OFFICES
city: OXFORD
postcode: OX1 2JD
website: www.ox.ac.uk

contact info
title: n.a.
name: n.a.
surname: n.a.
function: n.a.
email: n.a.
telephone: n.a.
fax: n.a.

 Coordinator Country United Kingdom [UK]
 Total cost 183˙454 €
 EC max contribution 183˙454 € (100%)
 Programme 1. H2020-EU.1.3.2. (Nurturing excellence by means of cross-border and cross-sector mobility)
 Code Call H2020-MSCA-IF-2017
 Funding Scheme MSCA-IF-EF-ST
 Starting year 2018
 Duration (year-month-day) from 2018-05-01   to  2020-04-30

 Partnership

Take a look of project's partnership.

# participants  country  role  EC contrib. [€] 
1    THE CHANCELLOR, MASTERS AND SCHOLARS OF THE UNIVERSITY OF OXFORD UK (OXFORD) coordinator 183˙454.00

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 Project objective

Recent studies have highlighted a pivotal role for long non-coding RNAs (lncRNAs) in normal physiology and disease. As a key example, Xist lncRNA is crucial during the early development of female mammals and is involved in many diseases, e.g. progeria and cancers. To date, cellular and developmental studies have advanced our knowledge of the function of Xist, but a detailed understanding of molecular mechanism is lacking.

Therefore, the overarching aim of my proposed research is to directly visualise Xist RNA/ ribonucleoprotein particles (RNPs) in cryo cells at sub-atomic resolution. To this end, I have formulated the following objectives: 1) preparing a cryo sample of mES cells expressing Xist RNA optimised for cryo electron tomography (ET); 2) visualising 3D-Xist RNPs in the mES cell; 3) generating homogenous pseudo-physiological Xist RNPs for single particle cryo-EM; 4) Solving the 3D sub-atomic resolution structure of Xist RNP; 5) Analysing a composite cryo-EM/-ET structure of the Xist RNPs in the mES cell.

To achieve this aim, I will employ an interdisciplinary approach including cutting-edge cryo-structural techniques and elegant mES cell biology. My supervisor Prof Brockdorff is a leading scientist in Xist lncRNA research and the host has a state-of-the-art cryo EM facility. The University of Oxford is a world-class research centre, providing excellent transferrable skills trainings and a fantastic infrastructure for career development.

The first high-resolution 3D structure of a lncRNA resulting from this innovative research will benefit not only the lncRNA research field, by providing novel molecular insight of the Xist lncRNA, but also the structural biology field, by expanding the 3D RNA structural potential. Dissemination and exploitation of this work, to be achieved by presenting in international conferences and publishing in high-impact open access journals, and communication, through public engagement, are an integral part of this proposed action.

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