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3D-Xist SIGNED

3D structure of the long non-coding RNA Xist by complementary cryo-electron tomography and single particle cryo-electron microscopy

Total Cost €

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EC-Contrib. €

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Partnership

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 3D-Xist project word cloud

Explore the words cloud of the 3D-Xist project. It provides you a very rough idea of what is the project "3D-Xist" about.

rnas    integral    prof    progeria    single    developmental    female    rnp    employ    engagement    cancers    visualising    highlighted    pseudo    class    excellent    diseases    host    supervisor    resolution    disease    homogenous    atomic    world    preparing    rna    scientist    publishing    expanding    elegant    xist    structure    date    electron    skills    brockdorff    interdisciplinary    cellular    transferrable    lncrnas    ing    presenting    first    et    3d    mammals    mes    composite    molecular    sub    edge    communication    expressing    generating    career    lacking    cryo    normal    coding    innovative    pivotal    infrastructure    ribonucleoprotein    journals    public    rnps    action    physiology    cells    mechanism    international    conferences    centre    recent    em    facility    particles    analysing    benefit    solving    cutting    techniques    structural    oxford    lncrna    biology    formulated    fantastic    trainings    particle    function    cell    university    visualise    physiological    overarching    tomography   

Project "3D-Xist" data sheet

The following table provides information about the project.

Coordinator
THE CHANCELLOR, MASTERS AND SCHOLARS OF THE UNIVERSITY OF OXFORD 

Organization address
address: WELLINGTON SQUARE UNIVERSITY OFFICES
city: OXFORD
postcode: OX1 2JD
website: www.ox.ac.uk

contact info
title: n.a.
name: n.a.
surname: n.a.
function: n.a.
email: n.a.
telephone: n.a.
fax: n.a.

 Coordinator Country United Kingdom [UK]
 Total cost 183˙454 €
 EC max contribution 183˙454 € (100%)
 Programme 1. H2020-EU.1.3.2. (Nurturing excellence by means of cross-border and cross-sector mobility)
 Code Call H2020-MSCA-IF-2017
 Funding Scheme MSCA-IF-EF-ST
 Starting year 2018
 Duration (year-month-day) from 2018-05-01   to  2020-04-30

 Partnership

Take a look of project's partnership.

# participants  country  role  EC contrib. [€] 
1    THE CHANCELLOR, MASTERS AND SCHOLARS OF THE UNIVERSITY OF OXFORD UK (OXFORD) coordinator 183˙454.00

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 Project objective

Recent studies have highlighted a pivotal role for long non-coding RNAs (lncRNAs) in normal physiology and disease. As a key example, Xist lncRNA is crucial during the early development of female mammals and is involved in many diseases, e.g. progeria and cancers. To date, cellular and developmental studies have advanced our knowledge of the function of Xist, but a detailed understanding of molecular mechanism is lacking.

Therefore, the overarching aim of my proposed research is to directly visualise Xist RNA/ ribonucleoprotein particles (RNPs) in cryo cells at sub-atomic resolution. To this end, I have formulated the following objectives: 1) preparing a cryo sample of mES cells expressing Xist RNA optimised for cryo electron tomography (ET); 2) visualising 3D-Xist RNPs in the mES cell; 3) generating homogenous pseudo-physiological Xist RNPs for single particle cryo-EM; 4) Solving the 3D sub-atomic resolution structure of Xist RNP; 5) Analysing a composite cryo-EM/-ET structure of the Xist RNPs in the mES cell.

To achieve this aim, I will employ an interdisciplinary approach including cutting-edge cryo-structural techniques and elegant mES cell biology. My supervisor Prof Brockdorff is a leading scientist in Xist lncRNA research and the host has a state-of-the-art cryo EM facility. The University of Oxford is a world-class research centre, providing excellent transferrable skills trainings and a fantastic infrastructure for career development.

The first high-resolution 3D structure of a lncRNA resulting from this innovative research will benefit not only the lncRNA research field, by providing novel molecular insight of the Xist lncRNA, but also the structural biology field, by expanding the 3D RNA structural potential. Dissemination and exploitation of this work, to be achieved by presenting in international conferences and publishing in high-impact open access journals, and communication, through public engagement, are an integral part of this proposed action.

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The information about "3D-XIST" are provided by the European Opendata Portal: CORDIS opendata.

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