Opendata, web and dolomites


The Quest of the Argonautes - from Myth to Reality

Total Cost €


EC-Contrib. €






 ARGO project word cloud

Explore the words cloud of the ARGO project. It provides you a very rough idea of what is the project "ARGO" about.

initially    strand    defence    genome    regulatory    argonautes    argonaute    engineering    pagos    catalyse    argo    breaks    architectures    laboratory    regulation    vitro    anti    expression    2010    dna    tip    synthetic    discoveries    distantly    biochemical    evolution    editing    small    nucleases    subunit    expedition    family    guided    domain    rnai    stranded    chromosome    rna    functional    iceberg    prokaryotic    players    discovered    spectacular    ligand    moderate    exploration    ing    temperatures    suggests    mesophilic    coding    stage    closely    cleavage    eukaryotes    thorough    interestingly    leap    original    eukaryotic    domains    team    functionalities    interference    silencing    complementary    prokaryotes    elevated    gene    base    mechanistic    pago    homologs    virus    revealed    tinkering    proteins    indicating    molecules    hence    cbago    thermophilic    organization    excellent    dissect    basis    unprecedented    double    embarking    variations    natural    elusive    mediator    possess    uncharacterized    characterization    random    guides    variants    big    generate    ago    rational    aforementioned    molecular   

Project "ARGO" data sheet

The following table provides information about the project.


Organization address
postcode: 6708 PB

contact info
title: n.a.
name: n.a.
surname: n.a.
function: n.a.
email: n.a.
telephone: n.a.
fax: n.a.

 Coordinator Country Netherlands [NL]
 Total cost 2˙177˙157 €
 EC max contribution 2˙177˙157 € (100%)
 Programme 1. H2020-EU.1.1. (EXCELLENT SCIENCE - European Research Council (ERC))
 Code Call ERC-2018-ADG
 Funding Scheme ERC-ADG
 Starting year 2019
 Duration (year-month-day) from 2019-07-01   to  2024-06-30


Take a look of project's partnership.

# participants  country  role  EC contrib. [€] 
1    WAGENINGEN UNIVERSITY NL (WAGENINGEN) coordinator 2˙177˙157.00


 Project objective

Argonaute nucleases are key players of the eukaryotic RNA interference (RNAi) system. Using small RNA guides, these Argonaute (Ago) proteins specifically target complementary RNA molecules, resulting in regulation of a wide range of crucial processes, including chromosome organization, gene expression and anti-virus defence. Since 2010, my research team has studied closely-related prokaryotic Argonaute (pAgo) variants. This has revealed spectacular mechanistic variations: several thermophilic pAgos catalyse DNA-guided cleavage of double stranded DNA, but only at elevated temperatures. Interestingly, a recently discovered mesophilic Argonaute (CbAgo) can generate double strand DNA breaks at moderate temperatures, providing an excellent basis for this ARGO project. In addition, genome analysis has revealed many distantly-related Argonaute variants, often with unique domain architectures. Hence, the currently known Argonaute homologs are just the tip of the iceberg, and the stage is set for making a big leap in the exploration of the Argonaute family. Initially we will dissect the molecular basis of functional and mechanistic features of uncharacterized natural Argonaute variants, both in eukaryotes (the presence of an Ago-like subunit in the Mediator complex, strongly suggests a regulatory role of an elusive non-coding RNA ligand) and in prokaryotes (selected Ago variants possess distinct domains indicating novel functionalities). After their thorough biochemical characterization, I aim at engineering the functionality of the aforementioned CbAgo through an integrated rational & random approach, i.e. by tinkering of domains, and by an unprecedented in vitro laboratory evolution approach. Eventually, natural & synthetic Argonautes will be selected for their exploitation, and used for developing original genome editing applications (from silencing to base editing). Embarking on this ambitious ARGO expedition will lead us to many exciting discoveries.

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The information about "ARGO" are provided by the European Opendata Portal: CORDIS opendata.

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