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ARGO SIGNED

The Quest of the Argonautes - from Myth to Reality

Total Cost €

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EC-Contrib. €

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Partnership

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 ARGO project word cloud

Explore the words cloud of the ARGO project. It provides you a very rough idea of what is the project "ARGO" about.

ing    discoveries    discovered    base    original    closely    uncharacterized    elevated    indicating    eukaryotic    molecules    thermophilic    random    architectures    argonautes    leap    initially    chromosome    elusive    laboratory    distantly    temperatures    iceberg    breaks    molecular    rational    virus    suggests    ago    engineering    homologs    strand    regulatory    guides    small    mesophilic    possess    proteins    moderate    excellent    team    gene    biochemical    pagos    rnai    big    argonaute    tinkering    revealed    cleavage    subunit    ligand    expedition    nucleases    interference    editing    hence    stage    spectacular    genome    basis    2010    defence    stranded    domains    anti    mediator    argo    cbago    double    family    eukaryotes    evolution    dissect    domain    mechanistic    aforementioned    unprecedented    functionalities    interestingly    thorough    embarking    functional    silencing    vitro    guided    prokaryotic    prokaryotes    tip    dna    expression    regulation    synthetic    players    exploration    catalyse    generate    rna    variations    complementary    characterization    coding    pago    variants    natural    organization   

Project "ARGO" data sheet

The following table provides information about the project.

Coordinator
WAGENINGEN UNIVERSITY 

Organization address
address: DROEVENDAALSESTEEG 4
city: WAGENINGEN
postcode: 6708 PB
website: http://www.wageningenur.nl/nl.htm

contact info
title: n.a.
name: n.a.
surname: n.a.
function: n.a.
email: n.a.
telephone: n.a.
fax: n.a.

 Coordinator Country Netherlands [NL]
 Total cost 2˙177˙157 €
 EC max contribution 2˙177˙157 € (100%)
 Programme 1. H2020-EU.1.1. (EXCELLENT SCIENCE - European Research Council (ERC))
 Code Call ERC-2018-ADG
 Funding Scheme ERC-ADG
 Starting year 2019
 Duration (year-month-day) from 2019-07-01   to  2024-06-30

 Partnership

Take a look of project's partnership.

# participants  country  role  EC contrib. [€] 
1    WAGENINGEN UNIVERSITY NL (WAGENINGEN) coordinator 2˙177˙157.00

Map

 Project objective

Argonaute nucleases are key players of the eukaryotic RNA interference (RNAi) system. Using small RNA guides, these Argonaute (Ago) proteins specifically target complementary RNA molecules, resulting in regulation of a wide range of crucial processes, including chromosome organization, gene expression and anti-virus defence. Since 2010, my research team has studied closely-related prokaryotic Argonaute (pAgo) variants. This has revealed spectacular mechanistic variations: several thermophilic pAgos catalyse DNA-guided cleavage of double stranded DNA, but only at elevated temperatures. Interestingly, a recently discovered mesophilic Argonaute (CbAgo) can generate double strand DNA breaks at moderate temperatures, providing an excellent basis for this ARGO project. In addition, genome analysis has revealed many distantly-related Argonaute variants, often with unique domain architectures. Hence, the currently known Argonaute homologs are just the tip of the iceberg, and the stage is set for making a big leap in the exploration of the Argonaute family. Initially we will dissect the molecular basis of functional and mechanistic features of uncharacterized natural Argonaute variants, both in eukaryotes (the presence of an Ago-like subunit in the Mediator complex, strongly suggests a regulatory role of an elusive non-coding RNA ligand) and in prokaryotes (selected Ago variants possess distinct domains indicating novel functionalities). After their thorough biochemical characterization, I aim at engineering the functionality of the aforementioned CbAgo through an integrated rational & random approach, i.e. by tinkering of domains, and by an unprecedented in vitro laboratory evolution approach. Eventually, natural & synthetic Argonautes will be selected for their exploitation, and used for developing original genome editing applications (from silencing to base editing). Embarking on this ambitious ARGO expedition will lead us to many exciting discoveries.

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The information about "ARGO" are provided by the European Opendata Portal: CORDIS opendata.

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