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ARGO SIGNED

The Quest of the Argonautes - from Myth to Reality

Total Cost €

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EC-Contrib. €

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Partnership

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 ARGO project word cloud

Explore the words cloud of the ARGO project. It provides you a very rough idea of what is the project "ARGO" about.

functional    organization    mesophilic    generate    big    embarking    indicating    thermophilic    cbago    guided    molecules    silencing    small    original    leap    architectures    family    defence    basis    2010    rational    vitro    tinkering    elusive    strand    random    rna    ligand    initially    regulatory    argonautes    eukaryotes    catalyse    eukaryotic    uncharacterized    functionalities    gene    characterization    pagos    tip    base    proteins    cleavage    team    players    expedition    discovered    distantly    guides    prokaryotic    unprecedented    dna    biochemical    engineering    regulation    nucleases    mediator    possess    aforementioned    exploration    ago    breaks    revealed    excellent    discoveries    double    elevated    temperatures    laboratory    subunit    prokaryotes    interestingly    interference    domain    editing    hence    thorough    argonaute    argo    evolution    pago    complementary    chromosome    molecular    stage    stranded    homologs    genome    rnai    variations    mechanistic    iceberg    coding    dissect    expression    moderate    suggests    closely    spectacular    variants    synthetic    anti    ing    natural    virus    domains   

Project "ARGO" data sheet

The following table provides information about the project.

Coordinator
WAGENINGEN UNIVERSITY 

Organization address
address: DROEVENDAALSESTEEG 4
city: WAGENINGEN
postcode: 6708 PB
website: http://www.wageningenur.nl/nl.htm

contact info
title: n.a.
name: n.a.
surname: n.a.
function: n.a.
email: n.a.
telephone: n.a.
fax: n.a.

 Coordinator Country Netherlands [NL]
 Total cost 2˙177˙157 €
 EC max contribution 2˙177˙157 € (100%)
 Programme 1. H2020-EU.1.1. (EXCELLENT SCIENCE - European Research Council (ERC))
 Code Call ERC-2018-ADG
 Funding Scheme ERC-ADG
 Starting year 2019
 Duration (year-month-day) from 2019-07-01   to  2024-06-30

 Partnership

Take a look of project's partnership.

# participants  country  role  EC contrib. [€] 
1    WAGENINGEN UNIVERSITY NL (WAGENINGEN) coordinator 2˙177˙157.00

Map

 Project objective

Argonaute nucleases are key players of the eukaryotic RNA interference (RNAi) system. Using small RNA guides, these Argonaute (Ago) proteins specifically target complementary RNA molecules, resulting in regulation of a wide range of crucial processes, including chromosome organization, gene expression and anti-virus defence. Since 2010, my research team has studied closely-related prokaryotic Argonaute (pAgo) variants. This has revealed spectacular mechanistic variations: several thermophilic pAgos catalyse DNA-guided cleavage of double stranded DNA, but only at elevated temperatures. Interestingly, a recently discovered mesophilic Argonaute (CbAgo) can generate double strand DNA breaks at moderate temperatures, providing an excellent basis for this ARGO project. In addition, genome analysis has revealed many distantly-related Argonaute variants, often with unique domain architectures. Hence, the currently known Argonaute homologs are just the tip of the iceberg, and the stage is set for making a big leap in the exploration of the Argonaute family. Initially we will dissect the molecular basis of functional and mechanistic features of uncharacterized natural Argonaute variants, both in eukaryotes (the presence of an Ago-like subunit in the Mediator complex, strongly suggests a regulatory role of an elusive non-coding RNA ligand) and in prokaryotes (selected Ago variants possess distinct domains indicating novel functionalities). After their thorough biochemical characterization, I aim at engineering the functionality of the aforementioned CbAgo through an integrated rational & random approach, i.e. by tinkering of domains, and by an unprecedented in vitro laboratory evolution approach. Eventually, natural & synthetic Argonautes will be selected for their exploitation, and used for developing original genome editing applications (from silencing to base editing). Embarking on this ambitious ARGO expedition will lead us to many exciting discoveries.

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The information about "ARGO" are provided by the European Opendata Portal: CORDIS opendata.

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lastchecktime (2022-05-29 12:48:57) correctly updated