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ARGO SIGNED

The Quest of the Argonautes - from Myth to Reality

Total Cost €

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EC-Contrib. €

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Partnership

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 ARGO project word cloud

Explore the words cloud of the ARGO project. It provides you a very rough idea of what is the project "ARGO" about.

discovered    pagos    ligand    domains    evolution    molecular    biochemical    temperatures    regulation    possess    synthetic    tip    generate    elusive    exploration    guides    organization    embarking    engineering    excellent    eukaryotic    domain    characterization    catalyse    expedition    random    molecules    mechanistic    functional    pago    thermophilic    cbago    natural    closely    subunit    double    argonaute    players    hence    variants    dna    interestingly    complementary    base    moderate    thorough    rna    2010    argo    dissect    gene    spectacular    original    argonautes    revealed    genome    tinkering    indicating    aforementioned    family    uncharacterized    basis    suggests    silencing    prokaryotic    guided    virus    stage    breaks    architectures    nucleases    team    leap    ago    laboratory    discoveries    mesophilic    distantly    stranded    coding    eukaryotes    rational    proteins    elevated    expression    big    prokaryotes    variations    chromosome    strand    anti    functionalities    initially    editing    mediator    regulatory    homologs    ing    iceberg    small    defence    rnai    interference    unprecedented    vitro    cleavage   

Project "ARGO" data sheet

The following table provides information about the project.

Coordinator
WAGENINGEN UNIVERSITY 

Organization address
address: DROEVENDAALSESTEEG 4
city: WAGENINGEN
postcode: 6708 PB
website: http://www.wageningenur.nl/nl.htm

contact info
title: n.a.
name: n.a.
surname: n.a.
function: n.a.
email: n.a.
telephone: n.a.
fax: n.a.

 Coordinator Country Netherlands [NL]
 Total cost 2˙177˙157 €
 EC max contribution 2˙177˙157 € (100%)
 Programme 1. H2020-EU.1.1. (EXCELLENT SCIENCE - European Research Council (ERC))
 Code Call ERC-2018-ADG
 Funding Scheme ERC-ADG
 Starting year 2019
 Duration (year-month-day) from 2019-07-01   to  2024-06-30

 Partnership

Take a look of project's partnership.

# participants  country  role  EC contrib. [€] 
1    WAGENINGEN UNIVERSITY NL (WAGENINGEN) coordinator 2˙177˙157.00

Map

 Project objective

Argonaute nucleases are key players of the eukaryotic RNA interference (RNAi) system. Using small RNA guides, these Argonaute (Ago) proteins specifically target complementary RNA molecules, resulting in regulation of a wide range of crucial processes, including chromosome organization, gene expression and anti-virus defence. Since 2010, my research team has studied closely-related prokaryotic Argonaute (pAgo) variants. This has revealed spectacular mechanistic variations: several thermophilic pAgos catalyse DNA-guided cleavage of double stranded DNA, but only at elevated temperatures. Interestingly, a recently discovered mesophilic Argonaute (CbAgo) can generate double strand DNA breaks at moderate temperatures, providing an excellent basis for this ARGO project. In addition, genome analysis has revealed many distantly-related Argonaute variants, often with unique domain architectures. Hence, the currently known Argonaute homologs are just the tip of the iceberg, and the stage is set for making a big leap in the exploration of the Argonaute family. Initially we will dissect the molecular basis of functional and mechanistic features of uncharacterized natural Argonaute variants, both in eukaryotes (the presence of an Ago-like subunit in the Mediator complex, strongly suggests a regulatory role of an elusive non-coding RNA ligand) and in prokaryotes (selected Ago variants possess distinct domains indicating novel functionalities). After their thorough biochemical characterization, I aim at engineering the functionality of the aforementioned CbAgo through an integrated rational & random approach, i.e. by tinkering of domains, and by an unprecedented in vitro laboratory evolution approach. Eventually, natural & synthetic Argonautes will be selected for their exploitation, and used for developing original genome editing applications (from silencing to base editing). Embarking on this ambitious ARGO expedition will lead us to many exciting discoveries.

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The information about "ARGO" are provided by the European Opendata Portal: CORDIS opendata.

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