NANOTUBE

Lateral diffusion in artificial lipid nanotubes

Coordinatore	INSTITUT CURIE    more  Organization address address: 26, rue d'Ulm city: PARIS postcode: 75248 contact info Titolo: Ms. Nome: Corinne Cognome: Cumin Email: send email Telefono: +33.1.56.24.66.20 Fax: +33.1.56.24.66.27
Nazionalità Coordinatore	France [FR]
Totale costo	30˙000 €
EC contributo	30˙000 €
Programma	FP7-PEOPLE Specific programme "People" implementing the Seventh Framework Programme of the European Community for research, technological development and demonstration activities (2007 to 2013)
Code Call	FP7-PEOPLE-2009-RG
Funding Scheme	MC-ERG
Anno di inizio	2009
Periodo (anno-mese-giorno)	2009-11-01   -   2011-10-31

 Partecipanti

#	participant	country	role	EC contrib. [€]
1	INSTITUT CURIE  Organization address address: 26, rue d'Ulm city: PARIS postcode: 75248 contact info Titolo: Ms. Nome: Corinne Cognome: Cumin Email: send email Telefono: +33.1.56.24.66.20 Fax: +33.1.56.24.66.27	FR (PARIS)	coordinator	30˙000.00

Mappa

 Word cloud

Esplora la "nuvola delle parole (Word Cloud) per avere un'idea di massima del progetto.

 Obiettivo del progetto (Objective)

'The aim of the proposed project is to understand how the morphology of the phospholipid membrane, and in particular tubular geometry and high curvature, affects the diffusion and trapping of lipids and membrane proteins in synaptic spines of neuronal cells. This will be achieved by using a simplified in vitro system reconstituting artificial tubular membrane where the geometrical, physical and chemical parameters of the membrane can be closely controlled and varied and the diffusion of individual molecules in the membrane can be followed by single particle tracking (SPT) in the fluorescence microscope. To achieve spatial resolution of ca. 30 nm, which is about 10 times better than the diffraction limit of conventional optical microscopy, we will use SPT and trajectory analysis of quantum dots (QD) – semiconductor nanocrystals with exceptionally bright and stable fluorescence. QDs will be coupled to lipid or protein components of the membrane by biotin-streptavidin or antigen-antibody linkage. The project represents the first effort to experimentally elucidate the role of membrane shape in regulation of lateral diffusion in neuronal structures by using a model membrane system. Based on the proposed experiments and simulations we expect to formulate a model for lateral diffusion that explicitly takes geometry into account. Current proposal is a part of the larger multidisciplinary project jointly implemented by neurobiologists, theoretical physicists and biophysicists. This project combines an original approach, powerful techniques, expertise of the fellow and dynamic scientific environment at the host Institute. This forms a solid basis for cutting-edge research to the benefit of both the fellow and the Community. Successful completion of this project will promote the fellow’s transition towards an independent researcher and will facilitate his professional integration in France.'