BASYNTHEC

Bacterial Synthetic Minimal Genomes for Biotechnology

Coordinatore	INSTITUT NATIONAL DE LA RECHERCHE AGRONOMIQUE    more  Organization address address: Rue De L'Universite 147 city: PARIS CEDEX 07 postcode: 75338 contact info Titolo: Dr. Nome: Philippe Cognome: Noirot Email: send email Telefono: -106 Fax: -113
Nazionalità Coordinatore	France [FR]
Sito del progetto	http://www.basynthec.eu/
Totale costo	4˙823˙326 €
EC contributo	2˙993˙162 €
Programma	FP7-KBBE Specific Programme "Cooperation": Food, Agriculture and Biotechnology
Code Call	FP7-KBBE-2009-3
Funding Scheme	CP-FP
Anno di inizio	2010
Periodo (anno-mese-giorno)	2010-06-01   -   2013-05-31

 Partecipanti

#	participant	country	role	EC contrib. [€]
1	INSTITUT NATIONAL DE LA RECHERCHE AGRONOMIQUE  Organization address address: Rue De L'Universite 147 city: PARIS CEDEX 07 postcode: 75338 contact info Titolo: Dr. Nome: Philippe Cognome: Noirot Email: send email Telefono: -106 Fax: -113	FR (PARIS CEDEX 07)	coordinator	893˙043.00
2	EIDGENOESSISCHE TECHNISCHE HOCHSCHULE ZURICH  Organization address address: Raemistrasse 101 city: ZUERICH postcode: 8092 contact info Titolo: Prof. Nome: Uwe Cognome: Sauer Email: send email Telefono: -744 Fax: -697	CH (ZUERICH)	participant	607˙418.00
3	Nome Ente NON disponibile  Organization address address: Domstrasse 11 city: GREIFSWALD postcode: 17487 contact info Titolo: Prof. Nome: Michael Cognome: Hecker Email: send email Telefono: -867985 Fax: -867987	DE (GREIFSWALD)	participant	339˙203.00
4	ACADEMISCH ZIEKENHUIS GRONINGEN  Organization address address: Hanzeplein 1 city: GRONINGEN postcode: 9713 GZ contact info Titolo: Prof. Nome: Jan Maarten Cognome: Van Dijl Email: send email Telefono: -3615206 Fax: -3619124	NL (GRONINGEN)	participant	326˙422.00
5	DSM Nutritional Products Ltd  Organization address address: Wurmisweg 576 city: Kaiseraugst postcode: 4303 contact info Titolo: Dr. Nome: Zoltán Cognome: Prágai Email: send email Telefono: -8158264 Fax: -8158660	CH (Kaiseraugst)	participant	306˙510.00
6	NOVOZYMES A/S  Organization address address: Krogshoejvej 36 city: BAGSVAERD postcode: 2880 contact info Titolo: Dr. Nome: Michael Cognome: Dolberg Rasmussen Email: send email Telefono: -44463911 Fax: -44467258	DK (BAGSVAERD)	participant	297˙712.00
7	INRA TRANSFERT S.A.  Organization address address: RUE DU DOCTEUR FINLAY 28 city: PARIS postcode: 75015 contact info Titolo: Ms. Nome: Marie Cognome: Dufourcq Email: send email Telefono: -124 Fax: -130	FR (PARIS)	participant	222˙854.00
8	THE UNIVERSITY OF CHICAGO  Organization address address: S ELLIS AVE 5801 city: Chicago postcode: 60637 contact info Titolo: Prof. Nome: Rick Cognome: Stevens Email: send email Telefono: -4259	US (Chicago)	participant	0.00

Mappa

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 Obiettivo del progetto (Objective)

'BaSynthec will combine computational and experimental biology approaches with novel high-throughput methodologies to reduce and modify à la carte the chromosome of Bacillus subtilis, a genetically tractable bacterium and one of the key microbes used as a Cell Factory in biotechnology. Simpler B. subtilis strains with reduced energy consumption for self maintenance will be designed and constructed by removing some potentially expensive cellular processes. The cells with the lowest experimentally determined waste of energy and with industrially relevant phenotypes will be engineered to reroute the flux devoted to biomass formation through rational modifications of the complex metabolic regulations, and will be used as biotechnological platforms to plug in synthetic modules. For this purpose, BaSynthec will develop a model-driven approach to design and engineer the strains with predetermined features, with a particular focus on unrestricted metabolic activity and the plug-in of synthetic functional modules. This strategy is based on the recent development of two complementary modelling approaches for B. subtilis: i) a genome-scale model of genetic and metabolic regulatory networks associated with a novel method called “Resource Balance Analysis” defining the formal background of model-based approaches for engineering strains; and ii) the development of a new genome-scale metabolic model of B. subtilis which is the most complete and accurate that exists today. Two pathways of high biotechnological relevance will be used for establishing the proof-of-principle of the assembly of functional synthetic modules: i) the vitamin B5 biosynthetic pathway, and ii) the secretion machinery for the export of extra-cellular enzymes. It is anticipated that validated simpler bacterial strains together with the modelling framework generated by BaSynthec will be used as generic biotechnological platforms to better control and exploit cell metabolism in industrial processes.'

Introduzione (Teaser)

Using microbes as cell factories is nothing new but an EU project has developed means to genetically streamline bacteria to produce vitamins and industrial proteins. Cutting out unnecessary DNA and inserting desired genes will enable researchers to create microbes with the lowest possible energy use but maximum productivity.