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[e-POM-Bioanal] SIGNED

Electrobioanalytical Applications of Polyoxometalates

Total Cost €

EC-Contrib. €

Partnership

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Outcomes and
results

[e-POM-Bioanal] project word cloud

Explore the words cloud of the [e-POM-Bioanal] project. It provides you a very rough idea of what is the project "[e-POM-Bioanal]" about.

nucleotide melting host labeled single incorporation dntps mutations platforms polymorphism anchoring fashion screening withstanding electrochemical probes parallelised sold curve snp strategy polymorphisms enzymatic genetic temperature ligase either dideoxynucleotide strategies pom reactions fellow elevated primer dna solid mediated surface detection polyoxometalate extension rapid primers array ddntps expertise pcr

Project "[e-POM-Bioanal]" data sheet

The following table provides information about the project.

Coordinator	UNIVERSITE PIERRE ET MARIE CURIE - PARIS 6 There are not information about this coordinator. Please contact Fabio for more information, thanks.
Coordinator Country	France [FR]
Project website	http://www.ipcm.fr
Total cost	185˙076 €
EC max contribution	185˙076 € (100%)
Programme	1. H2020-EU.1.3.2. (Nurturing excellence by means of cross-border and cross-sector mobility)
Code Call	H2020-MSCA-IF-2014
Funding Scheme	MSCA-IF-EF-ST
Starting year	2015
Duration (year-month-day)	from 2015-06-01 to 2017-05-31

Partnership

Take a look of project's partnership.

#	participants	country	role	EC contrib. [€]
1	SORBONNE UNIVERSITE SORBONNE UNIVERSITE Organization address address: 21 RUE DE L'ECOLE DE MEDECINE city: PARIS postcode: 75006 website: n.a. contact info title: n.a. name: n.a. surname: n.a. function: n.a. email: n.a. telephone: n.a. fax: n.a.	FR (PARIS)	coordinator	185˙076.00
2	UNIVERSITE PIERRE ET MARIE CURIE - PARIS 6 UNIVERSITE PIERRE ET MARIE CURIE - PARIS 6 Organization address address: Place Jussieu 4 city: PARIS postcode: 75252 website: www.upmc.fr contact info title: n.a. name: n.a. surname: n.a. function: n.a. email: n.a. telephone: n.a. fax: n.a.	FR (PARIS)	coordinator	0.00

Map

Project objective

The proposed work will exploit the expertise and know-how of the host institute and the fellow to develop a strategy for the detection and screening of genetic mutations in a highly parallelised fashion.The project has an overall objective to develop an electrochemical single nucleotide polymorphism (SNP) detection strategy by using polyoxometalate (POM) labeled DNA (including PCR primers, dNTPs and ddNTPs) for use in solid-phase melting curve analysis and sold-phase array based primer extension and solid phase ligase mediated detection. In order to achieve this, robust surface for anchoring of DNA probes capable of withstanding elevated temperature for enzymatic reactions either in solid phase melting curve analysis or single dideoxynucleotide incorporation will be developed. The proposed strategies will allow for highly cost-effective, rapid and user-friendly platforms for the screening of genetic mutations and single nucleotide polymorphisms.

Publications

List of publications.
year	authors and title	journal	last update
2015	Ahmed M. Debela, Mayreli Ortiz, Valerio Beni, Serge Thorimbert, Denis Lesage, Richard B. Cole, Ciara K. O\'Sullivan, Bernold Hasenknopf Biofunctionalization of Polyoxometalates with DNA Primers, Their Use in the Polymerase Chain Reaction (PCR) and Electrochemical Detection of PCR Products published pages: 17721-17727, ISSN: 0947-6539, DOI: 10.1002/chem.201502247	Chemistry - A European Journal 21/49	2019-06-17
2016	A. M. Debela, S. Thorimbert, B. Hasenknopf, C. K. O\'Sullivan, M. Ortiz Electrochemical primer extension for the detection of single nucleotide polymorphisms in the cardiomyopathy associated MYH7 gene published pages: 757-759, ISSN: 1359-7345, DOI: 10.1039/C5CC07762A	Chem. Commun. 52/4	2019-06-17
2017	Ciara K O\'Sullivan, Mayreli Ortiz, Ahmed Debela Mehdi, Marketa Svobodova, Serge Thorimbert, Denis Lasage, Richard Cole, Bernold Hasenknopf PCR Incorporation of Polyoxometalate Modified Deoxynucleotide Triphosphates and Their Application in Molecular Electrochemical Sensing of Yersinia pestis published pages: , ISSN: 0947-6539, DOI: 10.1002/chem.201701295	Chemistry - A European Journal	2019-06-17

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The information about "[E-POM-BIOANAL]" are provided by the European Opendata Portal: CORDIS opendata.