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G-EDIT SIGNED

Mechanisms of RNA-guided genome editing in eukaryotes

Total Cost €

EC-Contrib. €

Partnership

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Outcomes and
results

G-EDIT project word cloud

Explore the words cloud of the G-EDIT project. It provides you a very rough idea of what is the project "G-EDIT" about.

ing copy invasive right regulation generational representing subtraction coordinated precise nuclei insights editing scanning entire guiding content rearrangements epigenetic question types mediated interactions elimination excision cleavage offspring molecular transposons protozoa programmed provides rnas machinery components thought fantastic eukaryotic transport nucleic questions massively occurs serve maternal developmental genome acids population programming mechanisms eukaryotes chromosomal small rearranged cells ciliated orchestrate protein extensive form efficient place determines deletions defense organization first maternally trans dna time massive characterizing rna single somatic intriguing immune ciliates human germline cancer opportunity

Project "G-EDIT" data sheet

The following table provides information about the project.

Coordinator	UNIVERSITAET BERN Organization address address: HOCHSCHULSTRASSE 6 city: BERN postcode: 3012 website: http://www.unibe.ch contact info title: n.a. name: n.a. surname: n.a. function: n.a. email: n.a. telephone: n.a. fax: n.a.
Coordinator Country	Switzerland [CH]
Total cost	1˙953˙000 €
EC max contribution	1˙953˙000 € (100%)
Programme	1. H2020-EU.1.1. (EXCELLENT SCIENCE - European Research Council (ERC))
Code Call	ERC-2015-CoG
Funding Scheme	ERC-COG
Starting year	2016
Duration (year-month-day)	from 2016-05-01 to 2021-04-30

Partnership

Take a look of project's partnership.

#	participants	country	role	EC contrib. [€]
1	UNIVERSITAET BERN UNIVERSITAET BERN Organization address address: HOCHSCHULSTRASSE 6 city: BERN postcode: 3012 website: http://www.unibe.ch contact info title: n.a. name: n.a. surname: n.a. function: n.a. email: n.a. telephone: n.a. fax: n.a.	CH (BERN)	coordinator	1˙953˙000.00

Map

Project objective

The goal of this project is to contribute to our understanding of RNA-mediated epigenetic mechanisms of genome regulation in eukaryotes. Ciliated protozoa offer a fantastic opportunity to investigate the complex process of trans-generational programming of chromosomal rearrangements, which is thought to serve as a form of immune defense against invasive DNA. Developmental processes in ciliates include extensive rearrangements of the germline DNA, including elimination of transposons and the precise excision of numerous single-copy elements derived from transposons. This process is considered to be maternally controlled because the maternal genome provides essential information in the form of RNA that determines the offspring's genome content and organization. This programmed DNA subtraction, the so-called ‘RNA scanning’ process, is mediated by trans-generational comparison between the germline and the maternal somatic genome. One of the most intriguing questions is how a complex population of small RNAs representing the entire germline genome can be compared to the entire rearranged maternal genome, resulting in the efficient selection of germline-specific RNAs, which are able to target DNA deletions in the developing genome. All this occurs in a very short time and involves a massively coordinated transport of all the components between three types of nuclei. This project focuses on characterizing the molecular machinery that can orchestrate the massive genome rearrangements in ciliates through nucleic acids and protein interactions. It also addresses the question how RNA targets DNA cleavage at the right place. In addition, this project aims to investigate the role of RNA in guiding chromosomal rearrangements in other eukaryotic systems, particularly in human cancer cells where genome editing often occurs on a large scale. This work may be the first step in providing novel insights into the process of programmed DNA rearrangements in higher eukaryotes.

Publications

List of publications.
year	authors and title	journal	last update
2018	Aditi Singh, Adrienne Vancura, Rafal K. Woycicki, Daniel J. Hogan, Alan G. Hendrick, Mariusz Nowacki Determination of the presence of 5-methylcytosine in Paramecium tetraurelia published pages: e0206667, ISSN: 1932-6203, DOI: 10.1371/journal.pone.0206667	PLOS ONE 13/10	2019-10-09
2018	Cristina Hoehener, Iris Hug, Mariusz Nowacki Dicer-like Enzymes with Sequence Cleavage Preferences published pages: , ISSN: 0092-8674, DOI: 10.1016/j.cell.2018.02.029	Cell	2019-06-19
2017	Dominique I. Furrer, Estienne C. Swart, Matthias F. Kraft, Pamela Y. Sandoval, Mariusz Nowacki Two Sets of Piwi Proteins Are Involved in Distinct sRNA Pathways Leading to Elimination of Germline-Specific DNA published pages: 505-520, ISSN: 2211-1247, DOI: 10.1016/j.celrep.2017.06.050	Cell Reports 20/2	2019-06-19
2017	Allen, Sarah E.; Hug, Iris; Pabian, Sylwia; Rzeszutek, Iwona; Hoehener, Cristina; Nowacki, Mariusz Circular Concatemers of Ultra-Short DNA Segments Produce Regulatory RNAs published pages: , ISSN: 0092-8674, DOI: 10.7892/boris.97052	Cell 1	2019-06-19
2017	Estienne C. Swart, Cyril Denby Wilkes, Pamela Y. Sandoval, Cristina Hoehener, Aditi Singh, Dominique I. Furrer, Miroslav Arambasic, Michael Ignarski, Mariusz Nowacki Identification and analysis of functional associations among natural eukaryotic genome editing components published pages: 1374, ISSN: 2046-1402, DOI: 10.12688/f1000research.12121.1	F1000Research 6	2019-06-19

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