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MCNTMTDC SIGNED

Metabolic control on NF-κB activity during dendritic cells-mediated immune tolerance to tumours

Total Cost €

EC-Contrib. €

Partnership

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MCNTMTDC project word cloud

Explore the words cloud of the MCNTMTDC project. It provides you a very rough idea of what is the project "MCNTMTDC" about.

tolergenic ikkb vivo deletion professional suppression marrow dendritic chimeric kb protective combined nf context amp beta ampk presenting itgax melanocyte brafv600e undergo overcoming pseudo tolerance contribution ampka1 conditional delta consume mouse graft tadc correlated functional metabolic immune expression implication maturation transferred antigen give ikbkb understand absence responsible irradiated skin signalling strategies ikk possibly mice immunity regulators bone starvation mechanism generate sensor laboratory background pten vaccination activation cells melanoma kinase isolated links nutrient function unknown cancer tolerogenic host energy nutrients tumour amounts master dc mutant cultured upstream revealed malignant model phenotype

Project "MCNTMTDC" data sheet

The following table provides information about the project.

Coordinator	CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE CNRS Organization address address: RUE MICHEL ANGE 3 city: PARIS postcode: 75794 website: www.cnrs.fr contact info title: n.a. name: n.a. surname: n.a. function: n.a. email: n.a. telephone: n.a. fax: n.a.
Coordinator Country	France [FR]
Total cost	173˙076 €
EC max contribution	173˙076 € (100%)
Programme	1. H2020-EU.1.3.2. (Nurturing excellence by means of cross-border and cross-sector mobility)
Code Call	H2020-MSCA-IF-2015
Funding Scheme	MSCA-IF-EF-ST
Starting year	2017
Duration (year-month-day)	from 2017-09-01 to 2019-08-31

Partnership

Take a look of project's partnership.

#	participants	country	role	EC contrib. [€]
1	CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE CNRS CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE CNRS Organization address address: RUE MICHEL ANGE 3 city: PARIS postcode: 75794 website: www.cnrs.fr contact info title: n.a. name: n.a. surname: n.a. function: n.a. email: n.a. telephone: n.a. fax: n.a.	FR (PARIS)	coordinator	173˙076.00

Map

Project objective

BACKGROUND: Dendritic cells (DC) are professional antigen presenting cells able to provide protective immunity to cancer. DC undergo functional maturation that can lead to tolerogenic phenotype associated with immune-suppression in cancer by a still unknown mechanism. Recently the host laboratory revealed a new role of IKKβ/NF-kB signalling in the tolergenic function of DC. However, the upstream regulators of this signalling and its implication in cancer have not yet been addressed. Since malignant cells consume high amounts of nutrients and DC become tolerogenic in low nutrient conditions possibly under the control of the master energy sensor AMP kinase (AMPK), a pseudo-starvation state in tumour-associated DC (TADC) may be responsible of their tolerogenic activity. AIMS: To determine the metabolic role of AMPK and the potential links with IKKb/NF-kB signalling in the tolerogenic activity of TADC in melanoma context. METHODS: I will use mice with a deletion of IKKβ in DC (IkbkbΔItgax) generated in the host laboratory and will generate mice with a deletion of AMPK in DC (Ampka1ΔItgax). Isolated DC will be cultured in high/low nutrient conditions or in the presence/absence of melanoma cells. Activation of AMPK and IKKβ/NF-kB signalling will be analyzed in these conditions and correlated with DC maturation. TADC will be analyzed in vivo using a mouse melanoma model with conditional melanocyte-specific expression of BRafV600E combined with PTEN deletion. The contribution of IKKβ and AMPK activation in TADC during tumour development will be assessed using (i) a skin graft model from BRafV600E mutant mice to IkbkbΔItgax and Ampka1ΔItgax mice and (ii) chimeric mice generated with bone marrow cells from IkbkbΔItgax and IkbkbΔItgax mice transferred to irradiated BRafV600E mutant mice. EXPECTED RESULTS/IMPACT: To understand the tolerogenic activity of TADC, which will give insight into potential strategies for overcoming tumour-induced tolerance in DC vaccination approaches.

Publications

List of publications.
year	authors and title	journal	last update
2018	Ghislat G, Lawrence T Autophagy in dendritic cells. published pages: 944-952, ISSN: 2042-0226, DOI:	Cellular and molecular immunology 2018 Nov;15(11) Epub 2018 Mar 2	2020-02-19

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