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5D-NanoTrack SIGNED

Five-Dimensional Localization Microscopy for Sub-Cellular Dynamics

Total Cost €

EC-Contrib. €

Partnership

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5D-NanoTrack project word cloud

Explore the words cloud of the 5D-NanoTrack project. It provides you a very rough idea of what is the project "5D-NanoTrack" about.

multiple cells unprecedented grail 3d powerful temporal sub super diffractive ahead 3 markers create pave variety dimensions dynamic capability optical human dynamics volumetric observation imaging trade engineering dna organization color technique divided spatiotemporal uniquely tracking cellular microscopy psfs micro simultaneously inside chromatin live function methodology intrinsically inherent pairs plan appropriate extremely applicable emitters suitable dense tools spatial spread positions billion holy track point psf fundamental bottlenecks signal lack overcome multicolor biological efficacy resolution limited visualization base life cell speed revolutionized holds nanoscale critical offs 5d time spectral observe

Project "5D-NanoTrack" data sheet

The following table provides information about the project.

Coordinator	TECHNION - ISRAEL INSTITUTE OF TECHNOLOGY Organization address address: SENATE BUILDING TECHNION CITY city: HAIFA postcode: 32000 website: www.technion.ac.il contact info title: n.a. name: n.a. surname: n.a. function: n.a. email: n.a. telephone: n.a. fax: n.a.
Coordinator Country	Israel [IL]
Total cost	1˙802˙500 €
EC max contribution	1˙802˙500 € (100%)
Programme	1. H2020-EU.1.1. (EXCELLENT SCIENCE - European Research Council (ERC))
Code Call	ERC-2018-STG
Funding Scheme	ERC-STG
Starting year	2018
Duration (year-month-day)	from 2018-11-01 to 2023-10-31

Partnership

Take a look of project's partnership.

#	participants	country	role	EC contrib. [€]
1	TECHNION - ISRAEL INSTITUTE OF TECHNOLOGY TECHNION - ISRAEL INSTITUTE OF TECHNOLOGY Organization address address: SENATE BUILDING TECHNION CITY city: HAIFA postcode: 32000 website: www.technion.ac.il contact info title: n.a. name: n.a. surname: n.a. function: n.a. email: n.a. telephone: n.a. fax: n.a.	IL (HAIFA)	coordinator	1˙802˙500.00

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Project objective

The sub-cellular processes that control the most critical aspects of life occur in three-dimensions (3D), and are intrinsically dynamic. While super-resolution microscopy has revolutionized cellular imaging in recent years, our current capability to observe the dynamics of life on the nanoscale is still extremely limited, due to inherent trade-offs between spatial, temporal and spectral resolution using existing approaches.

We propose to develop and demonstrate an optical microscopy methodology that would enable live sub-cellular observation in unprecedented detail. Making use of multicolor 3D point-spread-function (PSF) engineering, a technique I have recently developed, we will be able to simultaneously track multiple markers inside live cells, at high speed and in five-dimensions (3D, time, and color).

Multicolor 3D PSF engineering holds the potential of being a uniquely powerful method for 5D tracking. However, it is not yet applicable to live-cell imaging, due to significant bottlenecks in optical engineering and signal processing, which we plan to overcome in this project. Importantly, we will also demonstrate the efficacy of our method using a challenging biological application: real-time visualization of chromatin dynamics - the spatiotemporal organization of DNA. This is a highly suitable problem due to its fundamental importance, its role in a variety of cellular processes, and the lack of appropriate tools for studying it. The project is divided into 3 aims: 1. Technology development: diffractive-element design for multicolor 3D PSFs. 2. System design: volumetric tracking of dense emitters. 3. Live-cell measurements: chromatin dynamics.

Looking ahead, here we create the imaging tools that pave the way towards the holy grail of chromatin visualization: dynamic observation of the 3D positions of the ~3 billion DNA base-pairs in a live human cell. Beyond that, our results will be applicable to numerous 3D micro/nanoscale tracking applications.

Publications

List of publications.
year	authors and title	journal	last update
2019	Eran Hershko, Lucien E. Weiss, Tomer Michaeli, Yoav Shechtman Multicolor localization microscopy and point-spread-function engineering by deep learning published pages: 6158, ISSN: 1094-4087, DOI: 10.1364/oe.27.006158	Optics Express 27/5	2019-05-28

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