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NewSpindleForce SIGNED

A new class of microtubules in the spindle exerting forces on kinetochores

Total Cost €

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EC-Contrib. €

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Partnership

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Project "NewSpindleForce" data sheet

The following table provides information about the project.

Coordinator
RUDER BOSKOVIC INSTITUTE 

Organization address
address: Bijenicka cesta 54
city: ZAGREB
postcode: 10000
website: www.irb.hr

contact info
title: n.a.
name: n.a.
surname: n.a.
function: n.a.
email: n.a.
telephone: n.a.
fax: n.a.

 Coordinator Country Croatia [HR]
 Project website http://tolic.irb.hr/erc-project
 Total cost 2˙150˙000 €
 EC max contribution 2˙150˙000 € (100%)
 Programme 1. H2020-EU.1.1. (EXCELLENT SCIENCE - European Research Council (ERC))
 Code Call ERC-2014-CoG
 Funding Scheme ERC-COG
 Starting year 2015
 Duration (year-month-day) from 2015-04-01   to  2020-03-31

 Partnership

Take a look of project's partnership.

# participants  country  role  EC contrib. [€] 
1    RUDER BOSKOVIC INSTITUTE HR (ZAGREB) coordinator 2˙150˙000.00

Map

 Project objective

At the onset of division the cell forms a spindle, a micro-machine made of microtubules, which divide the chromosomes by pulling on kinetochores, protein complexes on the chromosome. The central question in the field is how accurate chromosome segregation results from the interactions between kinetochores, microtubules and the associated proteins. According to the current paradigm, the forces on kinetochores are produced by k-fibers, bundles of microtubules extending between the spindle pole and the kinetochore. The proposed project is built upon a groundbreaking hypothesis that a new class of microtubules, which we term bridging microtubules, bridge sister kinetochores. Our preliminary results show that bridging microtubules are responsible for the positioning of kinetochores in HeLa and PtK1 cells. Bridging microtubules have not been studied before because this requires cutting-edge microscopy and laser microsurgery techniques. By applying these methods, with which I have extensive expertise, we will determine the organization of these microtubules, identify the proteins that link them with k-fibers, and uncover where and how the forces for kinetochore positioning and movement are generated. My strength is in taking an interdisciplinary approach, which I will use in this project by combining laser microsurgery with genetic perturbations, quantitative measurements of the responses and comparison with theoretical models. Understanding the role of bridging microtubules in force generation and chromosome movements will not only shed light on the mechanism of chromosome segregation, but may also increase the potential of mitotic anticancer strategies, as the spindle is a major target for chemotherapy. The proposed ERC funding is essential for the success of these timely and ambitious experiments, allowing me to strengthen my position as an international leader in research on cell division, thereby increasing Europe's foremost position in this field.

 Publications

year authors and title journal last update
List of publications.
2019 Iva M. Tolić, Maja Novak, Nenad Pavin
Helical Twist and Rotational Forces in the Mitotic Spindle
published pages: 132, ISSN: 2218-273X, DOI: 10.3390/biom9040132
Biomolecules 9/4 2019-08-06
2016 Iva M. Tolić, Nenad Pavin
Bridging the gap between sister kinetochores
published pages: 1169-1170, ISSN: 1538-4101, DOI: 10.1080/15384101.2016.1157976
Cell Cycle 15/9 2019-05-31
2017 Bruno Polak, Patrik Risteski, Sonja Lesjak, Iva M Tolić
PRC1‐labeled microtubule bundles and kinetochore pairs show one‐to‐one association in metaphase
published pages: 217-230, ISSN: 1469-221X, DOI: 10.15252/embr.201642650
EMBO reports 18/2 2019-05-31
2017 Iva M. Tolić
Mitotic spindle: kinetochore fibers hold on tight to interpolar bundles
published pages: , ISSN: 0175-7571, DOI: 10.1007/s00249-017-1244-4
European Biophysics Journal 2019-05-31
2015 Nenad Pavin, Iva M. Tolić
Self-Organization and Forces in the Mitotic Spindle
published pages: 279-298, ISSN: 1936-122X, DOI: 10.1146/annurev-biophys-062215-010934
Annual Review of Biophysics 45/1 2019-05-31
2016 Janko Kajtez, Anastasia Solomatina, Maja Novak, Bruno Polak, Kruno Vukušić, Jonas Rüdiger, Gheorghe Cojoc, Ana Milas, Ivana Šumanovac Šestak, Patrik Risteski, Federica Tavano, Anna H. Klemm, Emanuele Roscioli, Julie Welburn, Daniela Cimini, Matko Glunčić, Nenad Pavin, Iva M. Tolić
Overlap microtubules link sister k-fibres and balance the forces on bi-oriented kinetochores
published pages: 10298, ISSN: 2041-1723, DOI: 10.1038/ncomms10298
Nature Communications 7 2019-05-31
2018 Anna H. Klemm, Agneza Bosilj, Matko Gluncˇic´, Nenad Pavin, Iva M. Tolic´
Metaphase kinetochore movements are regulated by kinesin-8 motors and microtubule dynamic instability
published pages: 1332-1345, ISSN: 1059-1524, DOI: 10.1091/mbc.e17-11-0667
Molecular Biology of the Cell 29/11 2019-05-31
2017 R. Buđa, K. Vukušić, I.M. Tolić
Dissection and characterization of microtubule bundles in the mitotic spindle using femtosecond laser ablation.
published pages: 81-101, ISSN: , DOI: 10.1016/bs.mcb.2016.11.007
Methods in Cell Biology 139: 81–101 (2017) 2019-05-31
2016 Ana Milas, Iva Tolić
Relaxation of interkinetochore tension after severing of a k-fiber depends on the length of the k-fiber stub
published pages: , ISSN: 2297-9239, DOI: 10.19185/matters.201603000025
Matters Select Mar 23rd, 2016 2019-05-31

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