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EM-Pillars SIGNED

Controlled cryo-EM sample preparation through DNA-Origami pillars

Total Cost €

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EC-Contrib. €

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Partnership

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Project "EM-Pillars" data sheet

The following table provides information about the project.

Coordinator
MEDICAL RESEARCH COUNCIL 

Organization address
address: NORTH STAR AVENUE POLARIS HOUSE 2 FLOOR DAVID PHILLIPS BUILDING
city: SWINDON
postcode: SN2 1FL
website: www.mrc.ac.uk

contact info
title: n.a.
name: n.a.
surname: n.a.
function: n.a.
email: n.a.
telephone: n.a.
fax: n.a.

 Coordinator Country United Kingdom [UK]
 Project website http://www2.mrc-lmb.cam.ac.uk/
 Total cost 183˙454 €
 EC max contribution 183˙454 € (100%)
 Programme 1. H2020-EU.1.3.2. (Nurturing excellence by means of cross-border and cross-sector mobility)
 Code Call H2020-MSCA-IF-2014
 Funding Scheme MSCA-IF-EF-ST
 Starting year 2015
 Duration (year-month-day) from 2015-08-01   to  2017-07-31

 Partnership

Take a look of project's partnership.

# participants  country  role  EC contrib. [€] 
1    MEDICAL RESEARCH COUNCIL UK (SWINDON) coordinator 183˙454.00

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 Project objective

Electron cryomicroscopy (cryo-EM) is rapidly emerging as a powerful technique for high-resolution structure determination of protein complexes. Despite recent advances, there is still ample room for improvement of this method, in particular regarding sample preparation. Outstanding challenges are reproducibility of freezing conditions to obtain an optimal ice thickness; to prevent unfolding or preferential orientation of proteins against the hydrophobic air-water interface; and a limited accuracy of beam-induced motion correction for relatively small particles. Here, we propose to study how DNA origami objects that are added in with the sample may affect cryo-EM grid preparation. In addition, by examining a variety of DNA origami structures in the electron microscope, we also aim to identify and improve on structural weaknesses in their design. We will test new design strategies, analyse those, and use them for application in cryo-EM. Thereby, this proposal will facilitate both cryo-EM structure determination and the design of nano-tools that are made from DNA.

 Publications

year authors and title journal last update
List of publications.
2016 Thomas G. Martin, Tanmay A. M. Bharat, Andreas C. Joerger, Xiao-chen Bai, Florian Praetorius, Alan R. Fersht, Hendrik Dietz, Sjors H. W. Scheres
Design of a molecular support for cryo-EM structure determination
published pages: E7456-E7463, ISSN: 0027-8424, DOI: 10.1073/pnas.1612720113
Proceedings of the National Academy of Sciences 113/47 2019-06-14
2016 Monika Bokori-Brown, Thomas G. Martin, Claire E. Naylor, Ajit K. Basak, Richard W. Titball, Christos G. Savva
Cryo-EM structure of lysenin pore elucidates membrane insertion by an aerolysin family protein
published pages: 11293, ISSN: 2041-1723, DOI: 10.1038/ncomms11293
Nature Communications 7 2019-06-14

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