Opendata, web and dolomites

TWISTER

ToWards the Identification of Sesquiterpene TransportERs

Total Cost €

0

EC-Contrib. €

0

Partnership

0

Views

0

 TWISTER project word cloud

Explore the words cloud of the TWISTER project. It provides you a very rough idea of what is the project "TWISTER" about.

express    drug    defenses    metabolites    belong    sesquiterpene    glandular    specialized    binding    membranes    toxicity    family    direct    submitted    cassette    plants    putative    respectively    secondary    trichomes    poorly    despite    running    plant    instance    artemisia    interestingly    cells    membrane    extracted    aapdr1    potent    malaria    consists    extremely    vesicles    terpene    examine    molecules    compounds    acid    nicotiana    plasma    metabolisms    by2    surface    diterpene    sesquiterpenes    aapdr2    pdr    transport    aerial    anti    parallel    expressed    substrates    hypothesis    benefits    respective    annua    isolated    biosynthetic    notably    assays    shown    proteomic    transporters    deciphered    hypothesize    caryophyllene    structures    artemisinin    concerning    organs    precursor    quantitative    transportomics    pleiotropic    synthesize    dihydroartemisinic    shaped    szlig    rely    human    health    immense    resistance    tabacum    types    atp    terpenes    incorrect   

Project "TWISTER" data sheet

The following table provides information about the project.

Coordinator
UNIVERSITE CATHOLIQUE DE LOUVAIN 

Organization address
address: PLACE DE L UNIVERSITE 1
city: LOUVAIN LA NEUVE
postcode: 1348
website: www.uclouvain.be

contact info
title: n.a.
name: n.a.
surname: n.a.
function: n.a.
email: n.a.
telephone: n.a.
fax: n.a.

 Coordinator Country Belgium [BE]
 Project website https://uclouvain.be/fr/node/16698
 Total cost 160˙800 €
 EC max contribution 160˙800 € (100%)
 Programme 1. H2020-EU.1.3.2. (Nurturing excellence by means of cross-border and cross-sector mobility)
 Code Call H2020-MSCA-IF-2014
 Funding Scheme MSCA-IF-EF-ST
 Starting year 2015
 Duration (year-month-day) from 2015-08-01   to  2017-07-31

 Partnership

Take a look of project's partnership.

# participants  country  role  EC contrib. [€] 
1    UNIVERSITE CATHOLIQUE DE LOUVAIN BE (LOUVAIN LA NEUVE) coordinator 160˙800.00

Map

 Project objective

Plants synthesize a wide range of secondary metabolites, among them, terpenes. These molecules are notably produced in trichomes which are specialized structures present on the surface of aerial organs. Although the terpene biosynthetic pathways have been well deciphered, their transport across membranes remains poorly understood. This is for instance the case for sesquiterpenes such as artemisinin, an extremely potent anti-malaria drug. Despite the immense benefits of these compounds for plant defenses as well as for human health, no sesquiterpene transporters have yet been identified. It was recently shown that Pleiotropic Drug Resistance (PDR) transporters, which belong to the large ATP-Binding Cassette family, are involved in diterpene transport in Nicotiana tabacum trichomes. Thus, other PDR transporters could transport other types of terpenes such as sesquiterpenes. Recently, two PDR transporters of Artemisia annua, namely AaPDR1 and AaPDR2, have been shown to be specifically expressed in glandular and T-shaped trichomes, respectively. Interestingly, glandular trichomes are known to produce artemisinin while T-shaped trichomes have been shown to produce ß-caryophyllene. Thus, we can hypothesize that AaPDR1 and AaPDR2 transport dihydroartemisinic acid, the artemisinin precursor, and ß-caryophyllene, respectively. In order to examine this hypothesis, this project aims to express these transporters in N. tabacum BY2 cells and characterize their activity using toxicity and transport assays in whole cells as well as direct transport in plasma membrane vesicles. In case our hypothesis concerning the substrates is incorrect, we will rely on a transportomics approach, which consists of running transport assays using metabolites extracted from isolated trichomes as putative substrates. In parallel, as a side project, isolated glandular and T-shaped trichomes will be submitted to quantitative proteomic comparison in order to identify their respective metabolisms.

 Publications

year authors and title journal last update
List of publications.
2016 Bo Wang, Arman Beyraghdar Kashkooli, Adrienne Sallets, Hieng-Ming Ting, Norbert C.A. de Ruijter, Linda Olofsson, Peter Brodelius, Mathieu Pottier, Marc Boutry, Harro Bouwmeester, Alexander R. van der Krol
Transient production of artemisinin in Nicotiana benthamiana is boosted by a specific lipid transfer protein from A. annua
published pages: 159-169, ISSN: 1096-7176, DOI: 10.1016/j.ymben.2016.07.004
Metabolic Engineering 38 2019-07-24
2017 Raphaëlle Laterre, Mathieu Pottier, Claire Remacle, Marc Boutry
Photosynthetic Trichomes Contain a Specific Rubisco with a Modified pH-Dependent Activity
published pages: 2110-2120, ISSN: 0032-0889, DOI: 10.1104/pp.17.00062
Plant Physiology 173/4 2019-07-24

Are you the coordinator (or a participant) of this project? Plaese send me more information about the "TWISTER" project.

For instance: the website url (it has not provided by EU-opendata yet), the logo, a more detailed description of the project (in plain text as a rtf file or a word file), some pictures (as picture files, not embedded into any word file), twitter account, linkedin page, etc.

Send me an  email (fabio@fabiodisconzi.com) and I put them in your project's page as son as possible.

Thanks. And then put a link of this page into your project's website.

The information about "TWISTER" are provided by the European Opendata Portal: CORDIS opendata.

More projects from the same programme (H2020-EU.1.3.2.)

MingleIFT (2020)

Multi-color and single-molecule fluorescence imaging of intraflagellar transport in the phasmid chemosensory cilia of C. Elegans

Read More  

MathematicsAnalogies (2019)

Mathematics Analogies

Read More  

PNAIC (2018)

Positive and Negative Asymmetry in Intergroup Contact: Its Impact on Linguistic Forms of Communication and Physiological Responses

Read More