Explore the words cloud of the TWISTER project. It provides you a very rough idea of what is the project "TWISTER" about.
The following table provides information about the project.
UNIVERSITE CATHOLIQUE DE LOUVAIN
|Coordinator Country||Belgium [BE]|
|Total cost||160˙800 €|
|EC max contribution||160˙800 € (100%)|
1. H2020-EU.1.3.2. (Nurturing excellence by means of cross-border and cross-sector mobility)
|Duration (year-month-day)||from 2015-08-01 to 2017-07-31|
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|1||UNIVERSITE CATHOLIQUE DE LOUVAIN||BE (LOUVAIN LA NEUVE)||coordinator||160˙800.00|
Plants synthesize a wide range of secondary metabolites, among them, terpenes. These molecules are notably produced in trichomes which are specialized structures present on the surface of aerial organs. Although the terpene biosynthetic pathways have been well deciphered, their transport across membranes remains poorly understood. This is for instance the case for sesquiterpenes such as artemisinin, an extremely potent anti-malaria drug. Despite the immense benefits of these compounds for plant defenses as well as for human health, no sesquiterpene transporters have yet been identified. It was recently shown that Pleiotropic Drug Resistance (PDR) transporters, which belong to the large ATP-Binding Cassette family, are involved in diterpene transport in Nicotiana tabacum trichomes. Thus, other PDR transporters could transport other types of terpenes such as sesquiterpenes. Recently, two PDR transporters of Artemisia annua, namely AaPDR1 and AaPDR2, have been shown to be specifically expressed in glandular and T-shaped trichomes, respectively. Interestingly, glandular trichomes are known to produce artemisinin while T-shaped trichomes have been shown to produce ß-caryophyllene. Thus, we can hypothesize that AaPDR1 and AaPDR2 transport dihydroartemisinic acid, the artemisinin precursor, and ß-caryophyllene, respectively. In order to examine this hypothesis, this project aims to express these transporters in N. tabacum BY2 cells and characterize their activity using toxicity and transport assays in whole cells as well as direct transport in plasma membrane vesicles. In case our hypothesis concerning the substrates is incorrect, we will rely on a transportomics approach, which consists of running transport assays using metabolites extracted from isolated trichomes as putative substrates. In parallel, as a side project, isolated glandular and T-shaped trichomes will be submitted to quantitative proteomic comparison in order to identify their respective metabolisms.
|year||authors and title||journal||last update|
Bo Wang, Arman Beyraghdar Kashkooli, Adrienne Sallets, Hieng-Ming Ting, Norbert C.A. de Ruijter, Linda Olofsson, Peter Brodelius, Mathieu Pottier, Marc Boutry, Harro Bouwmeester, Alexander R. van der Krol
Transient production of artemisinin in Nicotiana benthamiana is boosted by a specific lipid transfer protein from A. annua
published pages: 159-169, ISSN: 1096-7176, DOI: 10.1016/j.ymben.2016.07.004
|Metabolic Engineering 38||2019-07-24|
RaphaÃ«lle Laterre, Mathieu Pottier, Claire Remacle, Marc Boutry
Photosynthetic Trichomes Contain a Specific Rubisco with a Modified pH-Dependent Activity
published pages: 2110-2120, ISSN: 0032-0889, DOI: 10.1104/pp.17.00062
|Plant Physiology 173/4||2019-07-24|
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