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3D Bioprinting of JOINT Replacements

Total Cost €


EC-Contrib. €






Project "3D-JOINT" data sheet

The following table provides information about the project.


Organization address
postcode: 3584 CX

contact info
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name: n.a.
surname: n.a.
function: n.a.
email: n.a.
telephone: n.a.
fax: n.a.

 Coordinator Country Netherlands [NL]
 Total cost 1˙998˙871 €
 EC max contribution 1˙998˙871 € (100%)
 Programme 1. H2020-EU.1.1. (EXCELLENT SCIENCE - European Research Council (ERC))
 Code Call ERC-2014-CoG
 Funding Scheme ERC-COG
 Starting year 2015
 Duration (year-month-day) from 2015-07-01   to  2020-06-30


Take a look of project's partnership.

# participants  country  role  EC contrib. [€] 
2    UNIVERSITEIT UTRECHT NL (UTRECHT) participant 97˙000.00


 Project objective

The world has a significant medical challenge in repairing injured or diseased joints. Joint degeneration and its related pain is a major socio-economic burden that will increase over the next decade and is currently addressed by implanting a metal prosthesis. For the long term, the ideal solution to joint injury is to successfully regenerate rather than replace the damaged cartilage with synthetic implants. Recent advances in key technologies are now bringing this “holy grail” within reach; regenerative approaches, based on cell therapy, are already clinically available albeit only for smaller focal cartilage defects. One of these key technologies is three-dimensional (3D) bio-printing, which provides a greatly controlled placement and organization of living constructs through the layer-by-layer deposition of materials and cells. These tissue constructs can be applied as tissue models for research and screening. However, the lack of biomechanical properties of these tissue constructs has hampered their application to the regeneration of damaged, degenerated or diseased tissue.

Having established a cartilage-focussed research laboratory in the University Medical Center Utrecht, I have addressed this biomechanical limitation of hydrogels through the use of hydrogel composites. Specifically, I have pioneered a 3D bio-printing technology that combines accurately printed small diameter thermoplast filaments with cell invasive hydrogels to form strong fibre-reinforced constructs. This, in combination with bioreactor technology, is the key to the generation of larger, complex tissue constructs with cartilage-like biomechanical resilience. With 3D-JOINT I will use my in-depth bio-printing and bioreactor knowledge and experience to develop a multi-phasic 3D-printed biological replacement of the joint.


year authors and title journal last update
List of publications.
2019 Paulina Nuñez Bernal, Paul Delrot, Damien Loterie, Yang Li, Jos Malda, Christophe Moser, Riccardo Levato
Volumetric Bioprinting of Complex Living‐Tissue Constructs within Seconds
published pages: 1904209, ISSN: 0935-9648, DOI: 10.1002/adma.201904209
Advanced Materials 31/42 2019-10-31
2019 Marleen Kristen, Madison J. Ainsworth, Nino Chirico, Casper F. T. Ven, Pieter A. Doevendans, Joost P. G. Sluijter, Jos Malda, Alain Mil, Miguel Castilho
Fiber Scaffold Patterning for Mending Hearts: 3D Organization Bringing the Next Step
published pages: 1900775, ISSN: 2192-2640, DOI: 10.1002/adhm.201900775
Advanced Healthcare Materials 2019-10-31
2019 Mylène de Ruijter, Alexandre Ribeiro, Inge Dokter, Miguel Castilho, Jos Malda
Simultaneous Micropatterning of Fibrous Meshes and Bioinks for the Fabrication of Living Tissue Constructs
published pages: 1800418, ISSN: 2192-2640, DOI: 10.1002/adhm.201800418
Advanced Healthcare Materials 8/7 2019-10-29
2019 I.A.D. Mancini, L. Rieppo, B. Pouran, I.O. Afara, F.M. Serra Braganca, M.H.P. van Rijen, M. Kik, H. Weinans, J. Toyras, P.R. van Weeren, J. Malda
Effects of body mass on microstructural features of the osteochondral unit: A comparative analysis of 37 mammalian species
published pages: 664-673, ISSN: 8756-3282, DOI: 10.1016/j.bone.2019.07.001
Bone 127 2019-10-29
2019 Miguel Castilho, Vivian Mouser, Mike Chen, Jos Malda, Keita Ito
Bi-layered micro-fibre reinforced hydrogels for articular cartilage regeneration
published pages: 297-306, ISSN: 1742-7061, DOI: 10.1016/j.actbio.2019.06.030
Acta Biomaterialia 95 2019-10-29
2018 Lorenzo Moroni, Thomas Boland, Jason A. Burdick, Carmelo De Maria, Brian Derby, Gabor Forgacs, Jürgen Groll, Qing Li, Jos Malda, Vladimir A. Mironov, Carlos Mota, Makoto Nakamura, Wenmiao Shu, Shoji Takeuchi, Tim B.F. Woodfield, Tao Xu, James J. Yoo, Giovanni Vozzi
Biofabrication: A Guide to Technology and Terminology
published pages: 384-402, ISSN: 0167-7799, DOI: 10.1016/j.tibtech.2017.10.015
Trends in Biotechnology 36/4 2019-10-29
2018 Katja Jansen, Miguel Castilho, Sanne Aarts, Michael M. Kaminski, Soeren S. Lienkamp, Roman Pichler, Jos Malda, Tina Vermonden, Jitske Jansen, Rosalinde Masereeuw
Fabrication of Kidney Proximal Tubule Grafts Using Biofunctionalized Electrospun Polymer Scaffolds
published pages: 1800412, ISSN: 1616-5187, DOI: 10.1002/mabi.201800412
Macromolecular Bioscience 19/2 2019-10-29
2018 Vivian Mouser
Ex vivo model unravelling cell distribution effect in hydrogels for cartilage repair
published pages: 65-76, ISSN: 1868-596X, DOI: 10.14573/altex.1704171
ALTEX 2019-10-29
2019 Jos Malda, Jürgen Groll, P. René van Weeren
Rethinking articular cartilage regeneration based on a 250-year-old statement
published pages: 571-572, ISSN: 1759-4790, DOI: 10.1038/s41584-019-0278-7
Nature Reviews Rheumatology 15/10 2019-10-29
2019 J. Boere, C.H.A. van de Lest, J.C. de Grauw, S.G.M. Plomp, S.F.W.M. Libregts, G.J.A. Arkesteijn, J. Malda, M.H.M. Wauben, P.R. van Weeren
Extracellular vesicles in synovial fluid from juvenile horses: No age-related changes in the quantitative profile
published pages: 91-93, ISSN: 1090-0233, DOI: 10.1016/j.tvjl.2018.12.010
The Veterinary Journal 244 2019-10-29
2018 Marcia de Almeida Monteiro Melo Ferraz, Heiko H. W. Henning, Pedro Ferreira da Costa, Jos Malda, Séverine Le Gac, Fabrice Bray, Majorie B. M. van Duursen, Jos F. Brouwers, Chris H. A. van de Lest, Ingeborg Bertijn, Lisa Kraneburg, Peter L. A. M. Vos, Tom A. E. Stout, Barend M. Gadella
Potential Health and Environmental Risks of Three-Dimensional Engineered Polymers
published pages: 80-85, ISSN: 2328-8930, DOI: 10.1021/acs.estlett.7b00495
Environmental Science & Technology Letters 5/2 2019-10-29
2018 Janneke Boere, Jos Malda, Chris H. A. van de Lest, P. René van Weeren, Marca H. M. Wauben
Extracellular Vesicles in Joint Disease and Therapy
published pages: , ISSN: 1664-3224, DOI: 10.3389/fimmu.2018.02575
Frontiers in Immunology 9 2019-10-29
2018 Jaakko K. Sarin, Nikae C. R. te Moller, Irina A. D. Mancini, Harold Brommer, Jetze Visser, Jos Malda, P. René van Weeren, Isaac O. Afara, Juha Töyräs
Arthroscopic near infrared spectroscopy enables simultaneous quantitative evaluation of articular cartilage and subchondral bone in vivo
published pages: , ISSN: 2045-2322, DOI: 10.1038/s41598-018-31670-5
Scientific Reports 8/1 2019-10-29
2019 J Groll, J A Burdick, D-W Cho, B Derby, M Gelinsky, S C Heilshorn, T Jüngst, J Malda, V A Mironov, K Nakayama, A Ovsianikov, W Sun, S Takeuchi, J J Yoo, T B F Woodfield
A definition of bioinks and their distinction from biomaterial inks
published pages: 13001, ISSN: 1758-5090, DOI: 10.1088/1758-5090/aaec52
Biofabrication 11/1 2019-10-29
2019 Barbara J. Klotz, Loes A. Oosterhoff, Lizette Utomo, Khoon S. Lim, Queralt Vallmajo‐Martin, Hans Clevers, Tim B. F. Woodfield, Antoine J. W. P. Rosenberg, Jos Malda, Martin Ehrbar, Bart Spee, Debby Gawlitta
A Versatile Biosynthetic Hydrogel Platform for Engineering of Tissue Analogues
published pages: 1900979, ISSN: 2192-2640, DOI: 10.1002/adhm.201900979
Advanced Healthcare Materials 8/19 2019-10-29
2017 Miguel Castilho, Dries Feyen, María Flandes-Iparraguirre, Gernot Hochleitner, Jürgen Groll, Pieter A. F. Doevendans, Tina Vermonden, Keita Ito, Joost P. G. Sluijter, Jos Malda
Melt Electrospinning Writing of Poly-Hydroxymethylglycolide- co -ε-Caprolactone-Based Scaffolds for Cardiac Tissue Engineering
published pages: 1700311, ISSN: 2192-2640, DOI: 10.1002/adhm.201700311
Advanced Healthcare Materials 6/18 2019-06-06
2016 Zita M. Jessop, Muhammad Javed, Iris A. Otto, Emman J. Combellack, Siân Morgan, Corstiaan C. Breugem, Charles W. Archer, Ilyas M. Khan, William C. Lineaweaver, Moshe Kon, Jos Malda, Iain S. Whitaker
Combining regenerative medicine strategies to provide durable reconstructive options: auricular cartilage tissue engineering
published pages: 7/19, ISSN: 1757-6512, DOI: 10.1186/s13287-015-0273-0
Stem Cell Research & Therapy 7/1 2019-06-06
2016 I A Otto, C C Breugem, J Malda, A L Bredenoord
Ethical considerations in the translation of regenerative biofabrication technologies into clinic and society
published pages: 42001, ISSN: 1758-5090, DOI: 10.1088/1758-5090/8/4/042001
Biofabrication 8/4 2019-06-06
2017 his piece was produced by Science Impact
Joint endeavours
published pages: , ISSN: , DOI:
2015 J. Visser, R. J. Custers, P. D. Dalton, R. Nizak, D. B.F. Saris, D. Gawlitta, J. Malda
Biofabrication of anatomically shaped implants for regeneration of the rabbit humeral head
published pages: , ISSN: , DOI:
2016 Anna Abbadessa, Vivian H. M. Mouser, Maarten M. Blokzijl, Debby Gawlitta, Wouter J. A. Dhert, Wim E. Hennink, Jos Malda, Tina Vermonden
A Synthetic Thermosensitive Hydrogel for Cartilage Bioprinting and Its Biofunctionalization with Polysaccharides
published pages: 2137-2147, ISSN: 1525-7797, DOI: 10.1021/acs.biomac.6b00366
Biomacromolecules 17/6 2019-06-06
2017 C. Villete, M. Castilho, J. Malda, A. Philips
Optimization of Heterogeneous Bone Tissue Engineering Scaffolds: Proof of Concept for a digital design tool.
published pages: , ISSN: , DOI:
2016 Vivian H M Mouser, Ferry P W Melchels, Jetze Visser, Wouter J A Dhert, Debby Gawlitta, Jos Malda
Yield stress determines bioprintability of hydrogels based on gelatin-methacryloyl and gellan gum for cartilage bioprinting
published pages: 35003, ISSN: 1758-5090, DOI: 10.1088/1758-5090/8/3/035003
Biofabrication 8/3 2019-06-06
2016 M. de Ruijter, A. Hrynevich, J.N. Haigh, M. Castilho, G. Hochleitner, J. Groll, J Malda, and P.D. Dalton
Melt electrospun fibers to improve shear properties
published pages: , ISSN: , DOI:
2016 R. Levato, W.R. Webb, I.A. Otto IA, V.H.M. Mouser, P.R. van Weeren, I.M. Khan, J. Malda
The bio in the ink: zonal-competent progenitor cells in printed layered constructs for cartilage regeneration
published pages: , ISSN: , DOI:
2017 Kerstin Schneeberger, Bart Spee, Pedro Costa, Norman Sachs, Hans Clevers, Jos Malda
Converging biofabrication and organoid technologies: the next frontier in hepatic and intestinal tissue engineering?
published pages: 13001, ISSN: 1758-5090, DOI: 10.1088/1758-5090/aa6121
Biofabrication 9/1 2019-06-06
2017 M. de Ruijter, A. Ribeiro, I. Dokter, M. Castilho, J. Malda
Converging of melt electrospinning writing and extrusion based bioprinting for cartilage tissue regeneration.
published pages: , ISSN: , DOI:
2017 M. de Ruijter, A. Hrynevich, J.N. Haigh, G. Hochleitner, M. Castilho, J. Malda
3D-Microfibers improve the shear modulus of hydrogel composites.
published pages: , ISSN: , DOI:
2017 C. Villete, M. Castilho, J. Malda, A. Philips
Optimised heterogeneous scaffolds: towards tailored bone mechanotransduction.
published pages: , ISSN: , DOI:
2016 Jos Malda, Janneke Boere, Chris H. A. van de Lest, P. René van Weeren, Marca H. M. Wauben
Extracellular vesicles — new tool for joint repair and regeneration
published pages: 243-249, ISSN: 1759-4790, DOI: 10.1038/nrrheum.2015.170
Nature Reviews Rheumatology 12/4 2019-06-06
2016 A. Abbadessa, M.M. Blokzijl, V.H.M. Mouser, P. Marica, J. Malda, W.E. Hennink, T. Vermonden
A thermo-responsive and photo-polymerizable chondroitin sulfate-based hydrogel for 3D printing applications
published pages: 163-174, ISSN: 0144-8617, DOI: 10.1016/j.carbpol.2016.04.080
Carbohydrate Polymers 149 2019-06-06
2016 M. de Ruijter, F.P.W Melchels, R. Levato, J. Malda
Hydrogel encapsulation and Micro-aggregation of co-culture MSC/AC for tissue regeneration.
published pages: , ISSN: , DOI:
2017 Riccardo Levato, William R. Webb, Iris A. Otto, Anneloes Mensinga, Yadan Zhang, Mattie van Rijen, René van Weeren, Ilyas M. Khan, Jos Malda
The bio in the ink: cartilage regeneration with bioprintable hydrogels and articular cartilage-derived progenitor cells
published pages: 41-53, ISSN: 1742-7061, DOI: 10.1016/j.actbio.2017.08.005
Acta Biomaterialia 61 2019-06-06
2017 R.A. Vindas Bolaños, S.M. Cokelaere, J.M. Estrada McDermott, K.E.M. Benders, U. Gbureck, S.G.M. Plomp, H. Weinans, J. Groll, P.R. van Weeren, J. Malda
The use of a cartilage decellularized matrix scaffold for the repair of osteochondral defects: the importance of long-term studies in a large animal model
published pages: 413-420, ISSN: 1063-4584, DOI: 10.1016/j.joca.2016.08.005
Osteoarthritis and Cartilage 25/3 2019-06-06
2017 M. Castilho, C. Moseke, U.Gbureck, E. Vorndran
3D printing of osteoconductive patient-specific bone implants.
published pages: , ISSN: , DOI:
2016 M. Castilho, G. Hochleitner, W. Wilson, J. Groll, J. Malda, K. Ito
Mechanical behaviour of soft hydrogels reinforced with printed microfiber scaffolds for cartilage repair.
published pages: , ISSN: , DOI:
2017 Pedro F. Costa, Hugo J. Albers, John E. A. Linssen, Heleen H. T. Middelkamp, Linda van der Hout, Robert Passier, Albert van den Berg, Jos Malda, Andries D. van der Meer
Mimicking arterial thrombosis in a 3D-printed microfluidic in vitro vascular model based on computed tomography angiography data
published pages: 2785-2792, ISSN: 1473-0197, DOI: 10.1039/c7lc00202e
Lab Chip 17/16 2019-06-06
2017 Wilhelmina Margaretha Groen, Paweena Diloksumpan, Paul René van Weeren, Riccardo Levato, Jos Malda
From intricate to integrated: Biofabrication of articulating joints
published pages: 2089-2097, ISSN: 0736-0266, DOI: 10.1002/jor.23602
Journal of Orthopaedic Research 35/10 2019-06-06
2015 Kristel W. M. Boere, Maarten M. Blokzijl, Jetze Visser, J. Elder A. Linssen, Jos Malda, Wim E. Hennink, Tina Vermonden
Biofabrication of reinforced 3D-scaffolds using two-component hydrogels
published pages: 9067-9078, ISSN: 2050-7518, DOI: 10.1039/c5tb01645b
Journal of Materials Chemistry B 3/46 2019-06-06
2016 K.S. Lim, R. Levato, P.F. Costa, M.D. Castilho, K. van Dorenmalen, F.P.W. Melchel, D. Gawlitta, J. Malda , T.B.F. Woodfield
Biofunctional and complex hydrogel architectures fabricated via visible light photo-crosslinking with digital light processing.
published pages: , ISSN: , DOI:
2017 M. Castilho, S. Kogelenberg, V. Mouser, J. Malda, K. Ito
Improving mechanical functioning of micro-fibre reinforced hydrogels for zonal articular cartilage repair.
published pages: , ISSN: , DOI:
2018 A Ribeiro, M M Blokzijl, R Levato, C W Visser, M Castilho, W E Hennink, T Vermonden, J Malda
Assessing bioink shape fidelity to aid material development in 3D bioprinting
published pages: 14102, ISSN: 1758-5090, DOI: 10.1088/1758-5090/aa90e2
Biofabrication 10/1 2019-06-06
2017 M. Castilho, S. van Kogelenberg, J. Malda, K. Ito
Improving mechanical functioning of micro-fibre reinforced hydrogels for cartilage repair.
published pages: , ISSN: , DOI:
2017 A. Ribeiro, M. Blokzijl, R. Levato, M. Castilho, W. Hennink, T. Vermonden, J. Malda
Assessing bioink printability with accessible and quantitative methods.
published pages: , ISSN: , DOI:
2016 M. Castilho, D. Feyen, M. Flandes-Iparraguirre, G. Hochleitner, P. Dalton, J. Groll, T. Vermonden, J. Malda, J. Sluijter
Melt electrospinning writing of poly-hydroxymethylglycolide-co-ε-caprolactone-based scaffolds for cardiac tissue engineering.
published pages: , ISSN: , DOI:
2017 Irina A.D. Mancini, Rafael A. Vindas Bolaños, Harold Brommer, Miguel Castilho, Alexandro Ribeiro, Johannes P.A.M. van Loon, Anneloes Mensinga, Mattie H.P. van Rijen, Jos Malda, René van Weeren
Fixation of Hydrogel Constructs for Cartilage Repair in the Equine Model: A Challenging Issue
published pages: 804-814, ISSN: 1937-3384, DOI: 10.1089/ten.TEC.2017.0200
Tissue Engineering Part C: Methods 23/11 2019-06-06
2017 Mylène de Ruijter, Andrei Hrynevich, Jodie N. Haigh, Gernot Hochleitner, Miguel Castilho, Jürgen Groll, Jos Malda, Paul D. Dalton
Out-of-Plane 3D-Printed Microfibers Improve the Shear Properties of Hydrogel Composites
published pages: 1702773, ISSN: 1613-6810, DOI: 10.1002/smll.201702773
Small 2019-06-06
2016 Vivian H. M. Mouser, Riccardo Levato, Lawrence J. Bonassar, Darryl D. D’Lima, Daniel A. Grande, Travis J. Klein, Daniel B. F. Saris, Marcy Zenobi-Wong, Debby Gawlitta, Jos Malda
Three-Dimensional Bioprinting and Its Potential in the Field of Articular Cartilage Regeneration
published pages: 327-340, ISSN: 1947-6035, DOI: 10.1177/1947603516665445
CARTILAGE 8/4 2019-06-06
2017 R. Levato, V. Mouser, W. Dhert, D. Gawlitta, R. van Weeren, J. Malda
Bioink development and role of cell-bioink interaction for the biofabrication.
published pages: , ISSN: , DOI:
2017 P. Diloksumpan, M. Castilho, T. Vermonden, R. van Weeren, J. Malda, R. Levato
Reinforcing hydrogel-to-ceramic interface in osteochondral constructs by converging additive manufacturing techniques.
published pages: , ISSN: , DOI:
2017 M. de Ruijter, A. Ribeiro, I. Dokter, M. Castilho, J. Malda
Converging MEW and extrusion-based bioprinting enables further mimicking of complex tissue architectures.
published pages: , ISSN: , DOI:
2017 M. Blokzijl, A. Ribeiro, C. Visser, R. Levato, M. Castilho, W. Hennink, T. Vermonden, J. Malda
Approach for standardized evaluation of printability and structural deformation of extruded bioink filaments.
published pages: , ISSN: , DOI:
2016 Ferry P W Melchels, Maarten M Blokzijl, Riccardo Levato, Quentin C Peiffer, Mylène de Ruijter, Wim E Hennink, Tina Vermonden, Jos Malda
Hydrogel-based reinforcement of 3D bioprinted constructs
published pages: 35004, ISSN: 1758-5090, DOI: 10.1088/1758-5090/8/3/035004
Biofabrication 8/3 2019-06-06
2016 Barbara J. Klotz, Debby Gawlitta, Antoine J.W.P. Rosenberg, Jos Malda, Ferry P.W. Melchels
Gelatin-Methacryloyl Hydrogels: Towards Biofabrication-Based Tissue Repair
published pages: 394-407, ISSN: 0167-7799, DOI: 10.1016/j.tibtech.2016.01.002
Trends in Biotechnology 34/5 2019-06-06
2017 R. Levato, I.A. Otto, W.R. Webb, R. Van Weeren, I.M. Khan, J. Malda
Application of different cell populations in hydrogel bioinks for zonal cartilage biofabrication.
published pages: , ISSN: , DOI:
2015 J. Visser, F. Melchels, I. Mancini, H. Brommer, P.Dalton, W.r Dhert, D. Saris, D. Hutmacher, R. van Weeren, J. Malda
Reinforced gelatin hydrogels for cartilage repair: from in vitro testing to implantation in the equine knee joint.
published pages: , ISSN: , DOI:
2016 F.P.W Melchels, K.E.C. Blokland, M. de Ruijter, J. Malda
A dual crosslinking strategy for reinforcing gelatine-methacrylamide hydrogels for tissue repair
published pages: , ISSN: , DOI:
2018 Miguel Castilho, Gernot Hochleitner, Wouter Wilson, Bert van Rietbergen, Paul D. Dalton, Jürgen Groll, Jos Malda, Keita Ito
Mechanical behavior of a soft hydrogel reinforced with three-dimensional printed microfibre scaffolds
published pages: 1245, ISSN: 2045-2322, DOI: 10.1038/s41598-018-19502-y
Scientific Reports 8/1 2019-06-06
2017 V H M Mouser, A Abbadessa, R Levato, W E Hennink, T Vermonden, D Gawlitta, J Malda
Development of a thermosensitive HAMA-containing bio-ink for the fabrication of composite cartilage repair constructs
published pages: 15026, ISSN: 1758-5090, DOI: 10.1088/1758-5090/aa6265
Biofabrication 9/1 2019-06-06
2017 M. de Ruijter, A. Hrynevich, J.N. Haigh, M. Castilho, G. Hochleitner, J. Groll, J Malda, and P.D. Dalton
Fabricatie van microvezels ter versterking van hydrogelen voor regeneratie van kraakbeen.
published pages: , ISSN: , DOI:

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