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Inter-bacterial competition mediated by the type VI secretion system (T6SS): promising role in the biocontrol properties of Pseudomonas putida

Total Cost €


EC-Contrib. €






 BIOCONT6SS project word cloud

Explore the words cloud of the BIOCONT6SS project. It provides you a very rough idea of what is the project "BIOCONT6SS" about.

effectors    functionally    putida    root    t6ss    immunity    pairs    assays    pathogenesis    components    plant    h3    prey    original    t6sss    prokaryotic    ei    characterise    biochemical    elucidation    agrobacterium    biology    h2    biocontrol    lack    cognate    members    soil    mutants    secretion    colonise    revealed    protection    determined    inter    structural    proteins    bacterial    model    belong    speculate    pathogens    h1    functional    kt2440    families    genome    discovered    bacteria    plants    tumefaciens    resilient    ralstonia    entirely    putative    phytopathogen    competition    fundamental    advantages    strains    nanomachines    phytopathogens    characterisation    colonisation    agent    pseudomonas    serious    environmental    solanacearum    infected    provides    play    microorganisms    agricultural    kill    syringae    eukaryotic    bacterium    existence    silico    crop    biotechnology    cells    characterised    vi    mainly    capacity    active    inject   

Project "BIOCONT6SS" data sheet

The following table provides information about the project.


Organization address
city: LONDON
postcode: SW7 2AZ

contact info
title: n.a.
name: n.a.
surname: n.a.
function: n.a.
email: n.a.
telephone: n.a.
fax: n.a.

 Coordinator Country United Kingdom [UK]
 Project website
 Total cost 195˙454 €
 EC max contribution 195˙454 € (100%)
 Programme 1. H2020-EU.1.3.2. (Nurturing excellence by means of cross-border and cross-sector mobility)
 Code Call H2020-MSCA-IF-2014
 Funding Scheme MSCA-IF-EF-ST
 Starting year 2016
 Duration (year-month-day) from 2016-09-01   to  2018-08-31


Take a look of project's partnership.

# participants  country  role  EC contrib. [€] 


 Project objective

Bacterial type VI secretion systems (T6SSs) are recently discovered nanomachines used to inject effectors into prokaryotic or eukaryotic cells. Therefore, T6SSs are involved in both inter-bacterial competition and bacterial pathogenesis. In this project, I propose to study the T6SS of Pseudomonas putida a soil bacterium with the capacity to colonise the root of crop plants. The colonisation by this bacterium provides growth advantages to the plant and, importantly, protection against plant-pathogens. This makes P. putida a relevant biocontrol agent. Since T6SS is mainly used by environmental bacteria for inter-bacterial competition, one might speculate that T6SSs play a relevant role in the biocontrol properties of P. putida. An in silico analysis of P. putida KT2440 genome revealed the existence of three putative T6SSs: H1, H2 and H3. I have determined that H1-T6SS is active and can be used to kill the serious phytopathogen Pseudomonas syringae. Mutants in H1-T6SS structural components lack the ability to kill model prey strains. In this project, I propose to use these mutants in competition assays with several microorganisms to further identify H1-T6SS targets, especially other resilient phytopathogens such as Agrobacterium tumefaciens and Ralstonia solanacearum. Furthermore, I will assess the role of T6SSs in the biocontrol properties of P. putida using infected plants. Moreover, I have identified novel H1-T6SSs effectors and their cognate immunity proteins (EI pairs) which belong to EI families without functionally characterised members. I will characterise the identified EI pairs at a functional, biochemical and structural biology level. In addition to the fundamental interest of the characterisation of new T6SSs, elucidation of their role in the biocontrol activity of P. putida is entirely original and has not been addressed thus far. Our findings will be highly relevant for future applications in agricultural biotechnology.


year authors and title journal last update
List of publications.
2018 Patricia Bernal, María A. Llamas, Alain Filloux
Type VI secretion systems in plant-associated bacteria
published pages: 1-15, ISSN: 1462-2912, DOI: 10.1111/1462-2920.13956
Environmental Microbiology 20/1 2019-04-03
2017 Patricia Bernal, Luke P Allsopp, Alain Filloux, María A Llamas
The Pseudomonas putida T6SS is a plant warden against phytopathogens
published pages: 972-987, ISSN: 1751-7362, DOI: 10.1038/ismej.2016.169
The ISME Journal 11/4 2019-04-03

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