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BIOCONT6SS

Inter-bacterial competition mediated by the type VI secretion system (T6SS): promising role in the biocontrol properties of Pseudomonas putida

Total Cost €

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EC-Contrib. €

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Partnership

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 BIOCONT6SS project word cloud

Explore the words cloud of the BIOCONT6SS project. It provides you a very rough idea of what is the project "BIOCONT6SS" about.

fundamental    biology    active    pseudomonas    effectors    serious    functionally    immunity    putida    eukaryotic    kt2440    silico    original    bacteria    nanomachines    provides    lack    existence    entirely    protection    phytopathogen    speculate    kill    h2    crop    play    components    elucidation    structural    biochemical    colonise    characterise    determined    model    biotechnology    pathogenesis    families    plants    colonisation    agricultural    characterised    h1    agent    genome    vi    plant    infected    putative    t6sss    resilient    mainly    competition    discovered    mutants    root    inter    solanacearum    phytopathogens    characterisation    secretion    soil    bacterial    pathogens    pairs    cognate    h3    functional    revealed    belong    biocontrol    ralstonia    prey    ei    strains    t6ss    prokaryotic    inject    advantages    tumefaciens    syringae    microorganisms    capacity    proteins    bacterium    assays    environmental    agrobacterium    members    cells   

Project "BIOCONT6SS" data sheet

The following table provides information about the project.

Coordinator
IMPERIAL COLLEGE OF SCIENCE TECHNOLOGY AND MEDICINE 

Organization address
address: SOUTH KENSINGTON CAMPUS EXHIBITION ROAD
city: LONDON
postcode: SW7 2AZ
website: http://www.imperial.ac.uk/

contact info
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surname: n.a.
function: n.a.
email: n.a.
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 Coordinator Country United Kingdom [UK]
 Project website http://www.imperial.ac.uk/people/p.bernal
 Total cost 195˙454 €
 EC max contribution 195˙454 € (100%)
 Programme 1. H2020-EU.1.3.2. (Nurturing excellence by means of cross-border and cross-sector mobility)
 Code Call H2020-MSCA-IF-2014
 Funding Scheme MSCA-IF-EF-ST
 Starting year 2016
 Duration (year-month-day) from 2016-09-01   to  2018-08-31

 Partnership

Take a look of project's partnership.

# participants  country  role  EC contrib. [€] 
1    IMPERIAL COLLEGE OF SCIENCE TECHNOLOGY AND MEDICINE UK (LONDON) coordinator 195˙454.00

Map

 Project objective

Bacterial type VI secretion systems (T6SSs) are recently discovered nanomachines used to inject effectors into prokaryotic or eukaryotic cells. Therefore, T6SSs are involved in both inter-bacterial competition and bacterial pathogenesis. In this project, I propose to study the T6SS of Pseudomonas putida a soil bacterium with the capacity to colonise the root of crop plants. The colonisation by this bacterium provides growth advantages to the plant and, importantly, protection against plant-pathogens. This makes P. putida a relevant biocontrol agent. Since T6SS is mainly used by environmental bacteria for inter-bacterial competition, one might speculate that T6SSs play a relevant role in the biocontrol properties of P. putida. An in silico analysis of P. putida KT2440 genome revealed the existence of three putative T6SSs: H1, H2 and H3. I have determined that H1-T6SS is active and can be used to kill the serious phytopathogen Pseudomonas syringae. Mutants in H1-T6SS structural components lack the ability to kill model prey strains. In this project, I propose to use these mutants in competition assays with several microorganisms to further identify H1-T6SS targets, especially other resilient phytopathogens such as Agrobacterium tumefaciens and Ralstonia solanacearum. Furthermore, I will assess the role of T6SSs in the biocontrol properties of P. putida using infected plants. Moreover, I have identified novel H1-T6SSs effectors and their cognate immunity proteins (EI pairs) which belong to EI families without functionally characterised members. I will characterise the identified EI pairs at a functional, biochemical and structural biology level. In addition to the fundamental interest of the characterisation of new T6SSs, elucidation of their role in the biocontrol activity of P. putida is entirely original and has not been addressed thus far. Our findings will be highly relevant for future applications in agricultural biotechnology.

 Publications

year authors and title journal last update
List of publications.
2018 Patricia Bernal, María A. Llamas, Alain Filloux
Type VI secretion systems in plant-associated bacteria
published pages: 1-15, ISSN: 1462-2912, DOI: 10.1111/1462-2920.13956
Environmental Microbiology 20/1 2019-04-03
2017 Patricia Bernal, Luke P Allsopp, Alain Filloux, María A Llamas
The Pseudomonas putida T6SS is a plant warden against phytopathogens
published pages: 972-987, ISSN: 1751-7362, DOI: 10.1038/ismej.2016.169
The ISME Journal 11/4 2019-04-03

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