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BIOCONT6SS

Inter-bacterial competition mediated by the type VI secretion system (T6SS): promising role in the biocontrol properties of Pseudomonas putida

Total Cost €

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EC-Contrib. €

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Partnership

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 BIOCONT6SS project word cloud

Explore the words cloud of the BIOCONT6SS project. It provides you a very rough idea of what is the project "BIOCONT6SS" about.

h2    bacterial    original    solanacearum    biocontrol    phytopathogens    effectors    strains    agrobacterium    kt2440    families    colonise    elucidation    kill    belong    inter    infected    tumefaciens    protection    assays    characterised    resilient    cells    serious    t6sss    prokaryotic    silico    immunity    genome    pairs    speculate    lack    determined    pathogenesis    active    provides    discovered    environmental    model    phytopathogen    colonisation    h3    nanomachines    existence    ralstonia    agricultural    capacity    play    syringae    pseudomonas    prey    biotechnology    agent    secretion    cognate    functionally    putida    competition    fundamental    plant    components    functional    biochemical    ei    mutants    h1    putative    members    vi    bacteria    mainly    biology    entirely    plants    t6ss    pathogens    root    soil    structural    crop    microorganisms    bacterium    characterise    eukaryotic    revealed    proteins    advantages    characterisation    inject   

Project "BIOCONT6SS" data sheet

The following table provides information about the project.

Coordinator
IMPERIAL COLLEGE OF SCIENCE TECHNOLOGY AND MEDICINE 

Organization address
address: SOUTH KENSINGTON CAMPUS EXHIBITION ROAD
city: LONDON
postcode: SW7 2AZ
website: http://www.imperial.ac.uk/

contact info
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name: n.a.
surname: n.a.
function: n.a.
email: n.a.
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 Coordinator Country United Kingdom [UK]
 Project website http://www.imperial.ac.uk/people/p.bernal
 Total cost 195˙454 €
 EC max contribution 195˙454 € (100%)
 Programme 1. H2020-EU.1.3.2. (Nurturing excellence by means of cross-border and cross-sector mobility)
 Code Call H2020-MSCA-IF-2014
 Funding Scheme MSCA-IF-EF-ST
 Starting year 2016
 Duration (year-month-day) from 2016-09-01   to  2018-08-31

 Partnership

Take a look of project's partnership.

# participants  country  role  EC contrib. [€] 
1    IMPERIAL COLLEGE OF SCIENCE TECHNOLOGY AND MEDICINE UK (LONDON) coordinator 195˙454.00

Map

 Project objective

Bacterial type VI secretion systems (T6SSs) are recently discovered nanomachines used to inject effectors into prokaryotic or eukaryotic cells. Therefore, T6SSs are involved in both inter-bacterial competition and bacterial pathogenesis. In this project, I propose to study the T6SS of Pseudomonas putida a soil bacterium with the capacity to colonise the root of crop plants. The colonisation by this bacterium provides growth advantages to the plant and, importantly, protection against plant-pathogens. This makes P. putida a relevant biocontrol agent. Since T6SS is mainly used by environmental bacteria for inter-bacterial competition, one might speculate that T6SSs play a relevant role in the biocontrol properties of P. putida. An in silico analysis of P. putida KT2440 genome revealed the existence of three putative T6SSs: H1, H2 and H3. I have determined that H1-T6SS is active and can be used to kill the serious phytopathogen Pseudomonas syringae. Mutants in H1-T6SS structural components lack the ability to kill model prey strains. In this project, I propose to use these mutants in competition assays with several microorganisms to further identify H1-T6SS targets, especially other resilient phytopathogens such as Agrobacterium tumefaciens and Ralstonia solanacearum. Furthermore, I will assess the role of T6SSs in the biocontrol properties of P. putida using infected plants. Moreover, I have identified novel H1-T6SSs effectors and their cognate immunity proteins (EI pairs) which belong to EI families without functionally characterised members. I will characterise the identified EI pairs at a functional, biochemical and structural biology level. In addition to the fundamental interest of the characterisation of new T6SSs, elucidation of their role in the biocontrol activity of P. putida is entirely original and has not been addressed thus far. Our findings will be highly relevant for future applications in agricultural biotechnology.

 Publications

year authors and title journal last update
List of publications.
2018 Patricia Bernal, María A. Llamas, Alain Filloux
Type VI secretion systems in plant-associated bacteria
published pages: 1-15, ISSN: 1462-2912, DOI: 10.1111/1462-2920.13956
Environmental Microbiology 20/1 2019-04-03
2017 Patricia Bernal, Luke P Allsopp, Alain Filloux, María A Llamas
The Pseudomonas putida T6SS is a plant warden against phytopathogens
published pages: 972-987, ISSN: 1751-7362, DOI: 10.1038/ismej.2016.169
The ISME Journal 11/4 2019-04-03

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