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illumizymes SIGNED

Illuminating aptamers and ribozymes for biomolecular tagging and fluorogen activation

Total Cost €

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EC-Contrib. €

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Partnership

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 illumizymes project word cloud

Explore the words cloud of the illumizymes project. It provides you a very rough idea of what is the project "illumizymes" about.

chromophores    metabolism    building    blocks    enrichment    posttranscriptional    systematic    vivo    visualization    reactions    functional    molecular    extra    inspired    noncoding    diverse    aptamers    vitro    select    flexibility    rnas    living    cells    cellular    relative    disciplines    sequences    small    ligand    naturally    proteins    activate    imaging    bio    poorly    repertoire    tools    probes    surprising    despite    attach    genome    health    complexity    binding    disease    evolve    restricting    catalytic    bonds    ribozymes    unknown    fluorophores    regulatory    types    enlighten    encodable    covalent    modifications    limited    brightness    prominent    add    permeable    consist    evolution    interrogation    rna    transcribed    toxic    fold    linear    photostability    conformational    biopolymers    dimensional    actual    endowed    orthogonal    structures    genetically    microscopy    fluorescence    roles    layer    composition    human    switching    fraction    manipulation    cell    latent    stable    selex    labels    exponential    tags    selective    illumizymes    ligands    labeling    introduce    functions    methodological    translated    color    affinity    fixed    simplicity    characterization    regulators   

Project "illumizymes" data sheet

The following table provides information about the project.

Coordinator
JULIUS-MAXIMILIANS-UNIVERSITAT WURZBURG 

Organization address
address: SANDERRING 2
city: WUERZBURG
postcode: 97070
website: http://www.uni-wuerzburg.de

contact info
title: n.a.
name: n.a.
surname: n.a.
function: n.a.
email: n.a.
telephone: n.a.
fax: n.a.

 Coordinator Country Germany [DE]
 Total cost 2˙061˙250 €
 EC max contribution 2˙061˙250 € (100%)
 Programme 1. H2020-EU.1.1. (EXCELLENT SCIENCE - European Research Council (ERC))
 Code Call ERC-2015-CoG
 Funding Scheme ERC-COG
 Starting year 2016
 Duration (year-month-day) from 2016-07-01   to  2021-06-30

 Partnership

Take a look of project's partnership.

# participants  country  role  EC contrib. [€] 
1    JULIUS-MAXIMILIANS-UNIVERSITAT WURZBURG DE (WUERZBURG) coordinator 1˙977˙500.00
2    GEORG-AUGUST-UNIVERSITAT GOTTINGENSTIFTUNG OFFENTLICHEN RECHTS DE (GOTTINGEN) participant 83˙750.00

Map

 Project objective

RNAs are linear biopolymers that consist of only four types of building blocks, but can fold into complex three-dimensional structures that are endowed with selective, high-affinity ligand-binding abilities known as aptamers, and catalytic activities known as ribozymes. Genome projects have brought the surprising insight that a large part of the human genome is transcribed into RNAs, but only a very small fraction is translated into proteins. The actual number of noncoding RNAs with specific functions is currently unknown, but many are considered as prominent regulators of cellular functions. Posttranscriptional modifications of RNA add an extra layer of complexity, but their regulatory roles in RNA metabolism are only poorly understood. Despite the relative simplicity in molecular composition, the available methodological repertoire for manipulation, interrogation and visualization of RNA is rather limited. This project aims to solve the challenge of RNA labeling in both fixed and living cells, using aptamers and ribozymes for RNA imaging and functional characterization. We introduce the term illumizymes for novel tools that attach bio-orthogonal tags and fluorophores to specific RNA sequences, in vitro and in vivo. Ribozymes and aptamers for small, stable, and specific labels will be identified by in vitro selection and systematic evolution of ligands by exponential enrichment (SELEX) to activate the fluorescence of latent chromophores by restricting their conformational flexibility and/or formation of covalent bonds by new bio-orthogonal reactions. Using naturally inspired, cell-permeable and non-toxic ligands, we will specifically select for increased brightness and photostability, and evolve illumizymes into color-switching probes for RNA microscopy. The new genetically encodable RNA devices will find widespread applications in diverse disciplines to enlighten our understanding of cellular RNA functions in health and disease.

 Publications

year authors and title journal last update
List of publications.
2019 Christian Steinmetzger, Irene Bessi, Ann-Kathrin Lenz, Claudia Höbartner
Structure–fluorescence activation relationships of a large Stokes shift fluorogenic RNA aptamer
published pages: 2019, 47, 11538-, ISSN: 0305-1048, DOI: 10.1093/nar/gkz1084
Nucleic Acids Research 2020-01-30
2018 Maksim V. Sednev, Volodymyr Mykhailiuk, Priyanka Choudhury, Julia Halang, Katherine E. Sloan, Markus T. Bohnsack, Claudia Höbartner
N 6 -Methyladenosine-Sensitive RNA-Cleaving Deoxyribozymes
published pages: 15117-15121, ISSN: 1433-7851, DOI: 10.1002/anie.201808745
Angewandte Chemie International Edition 57/46 2019-02-25
2017 Tucker J. Carrocci, Lea Lohe, Matthew J. Ashton, Claudia Höbartner, Aaron A. Hoskins
Debranchase-resistant labeling of RNA using the 10DM24 deoxyribozyme and fluorescent modified nucleotides
published pages: 11992-11995, ISSN: 1359-7345, DOI: 10.1039/c7cc06703h
Chemical Communications 53/88 2019-02-25
2018 Christian Steinmetzger, Navaneethan Palanisamy, Kiran R. Gore, Claudia Höbartner
A Multicolor Large Stokes Shift Fluorogen-Activating RNA Aptamer with Cationic Chromophores
published pages: 5, ISSN: 0947-6539, DOI: 10.1002/chem.201805882
Chemistry - A European Journal weekly 2019-02-25

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