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illumizymes SIGNED

Illuminating aptamers and ribozymes for biomolecular tagging and fluorogen activation

Total Cost €


EC-Contrib. €






 illumizymes project word cloud

Explore the words cloud of the illumizymes project. It provides you a very rough idea of what is the project "illumizymes" about.

microscopy    naturally    actual    living    fluorophores    tools    photostability    repertoire    proteins    translated    enrichment    characterization    orthogonal    evolve    binding    cellular    extra    molecular    poorly    rnas    introduce    vitro    fluorescence    ribozymes    systematic    disciplines    health    human    consist    diverse    relative    genome    types    complexity    regulators    catalytic    bio    functions    chromophores    noncoding    labels    despite    unknown    blocks    attach    prominent    brightness    genetically    endowed    modifications    exponential    illumizymes    ligands    cell    layer    color    stable    sequences    enlighten    cells    functional    encodable    building    latent    interrogation    rna    methodological    select    limited    aptamers    affinity    add    biopolymers    covalent    fold    transcribed    toxic    simplicity    tags    disease    small    visualization    structures    switching    metabolism    fixed    dimensional    fraction    composition    ligand    linear    vivo    selective    flexibility    labeling    permeable    probes    evolution    conformational    manipulation    activate    posttranscriptional    inspired    surprising    selex    restricting    regulatory    bonds    roles    reactions    imaging   

Project "illumizymes" data sheet

The following table provides information about the project.


Organization address
address: SANDERRING 2
postcode: 97070

contact info
title: n.a.
name: n.a.
surname: n.a.
function: n.a.
email: n.a.
telephone: n.a.
fax: n.a.

 Coordinator Country Germany [DE]
 Total cost 2˙061˙250 €
 EC max contribution 2˙061˙250 € (100%)
 Programme 1. H2020-EU.1.1. (EXCELLENT SCIENCE - European Research Council (ERC))
 Code Call ERC-2015-CoG
 Funding Scheme ERC-COG
 Starting year 2016
 Duration (year-month-day) from 2016-07-01   to  2021-06-30


Take a look of project's partnership.

# participants  country  role  EC contrib. [€] 


 Project objective

RNAs are linear biopolymers that consist of only four types of building blocks, but can fold into complex three-dimensional structures that are endowed with selective, high-affinity ligand-binding abilities known as aptamers, and catalytic activities known as ribozymes. Genome projects have brought the surprising insight that a large part of the human genome is transcribed into RNAs, but only a very small fraction is translated into proteins. The actual number of noncoding RNAs with specific functions is currently unknown, but many are considered as prominent regulators of cellular functions. Posttranscriptional modifications of RNA add an extra layer of complexity, but their regulatory roles in RNA metabolism are only poorly understood. Despite the relative simplicity in molecular composition, the available methodological repertoire for manipulation, interrogation and visualization of RNA is rather limited. This project aims to solve the challenge of RNA labeling in both fixed and living cells, using aptamers and ribozymes for RNA imaging and functional characterization. We introduce the term illumizymes for novel tools that attach bio-orthogonal tags and fluorophores to specific RNA sequences, in vitro and in vivo. Ribozymes and aptamers for small, stable, and specific labels will be identified by in vitro selection and systematic evolution of ligands by exponential enrichment (SELEX) to activate the fluorescence of latent chromophores by restricting their conformational flexibility and/or formation of covalent bonds by new bio-orthogonal reactions. Using naturally inspired, cell-permeable and non-toxic ligands, we will specifically select for increased brightness and photostability, and evolve illumizymes into color-switching probes for RNA microscopy. The new genetically encodable RNA devices will find widespread applications in diverse disciplines to enlighten our understanding of cellular RNA functions in health and disease.


year authors and title journal last update
List of publications.
2019 Christian Steinmetzger, Irene Bessi, Ann-Kathrin Lenz, Claudia Höbartner
Structure–fluorescence activation relationships of a large Stokes shift fluorogenic RNA aptamer
published pages: 2019, 47, 11538-, ISSN: 0305-1048, DOI: 10.1093/nar/gkz1084
Nucleic Acids Research 2020-01-30
2018 Maksim V. Sednev, Volodymyr Mykhailiuk, Priyanka Choudhury, Julia Halang, Katherine E. Sloan, Markus T. Bohnsack, Claudia Höbartner
N 6 -Methyladenosine-Sensitive RNA-Cleaving Deoxyribozymes
published pages: 15117-15121, ISSN: 1433-7851, DOI: 10.1002/anie.201808745
Angewandte Chemie International Edition 57/46 2019-02-25
2017 Tucker J. Carrocci, Lea Lohe, Matthew J. Ashton, Claudia Höbartner, Aaron A. Hoskins
Debranchase-resistant labeling of RNA using the 10DM24 deoxyribozyme and fluorescent modified nucleotides
published pages: 11992-11995, ISSN: 1359-7345, DOI: 10.1039/c7cc06703h
Chemical Communications 53/88 2019-02-25
2018 Christian Steinmetzger, Navaneethan Palanisamy, Kiran R. Gore, Claudia Höbartner
A Multicolor Large Stokes Shift Fluorogen-Activating RNA Aptamer with Cationic Chromophores
published pages: 5, ISSN: 0947-6539, DOI: 10.1002/chem.201805882
Chemistry - A European Journal weekly 2019-02-25

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