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illumizymes SIGNED

Illuminating aptamers and ribozymes for biomolecular tagging and fluorogen activation

Total Cost €

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EC-Contrib. €

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Partnership

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 illumizymes project word cloud

Explore the words cloud of the illumizymes project. It provides you a very rough idea of what is the project "illumizymes" about.

selective    restricting    proteins    vivo    repertoire    types    affinity    fixed    consist    small    enlighten    regulatory    interrogation    complexity    enrichment    switching    bio    translated    extra    limited    labels    probes    diverse    disciplines    encodable    imaging    visualization    layer    endowed    chromophores    inspired    toxic    reactions    noncoding    color    simplicity    molecular    vitro    living    add    transcribed    ligand    health    covalent    fluorophores    tools    rna    blocks    conformational    exponential    introduce    cell    actual    select    activate    human    brightness    fold    linear    fraction    regulators    disease    metabolism    cells    systematic    characterization    orthogonal    sequences    unknown    surprising    building    poorly    tags    genetically    binding    ligands    genome    evolve    dimensional    composition    biopolymers    ribozymes    illumizymes    structures    fluorescence    photostability    manipulation    cellular    despite    bonds    flexibility    labeling    stable    functional    modifications    attach    prominent    functions    permeable    microscopy    rnas    aptamers    evolution    relative    selex    posttranscriptional    latent    methodological    roles    naturally    catalytic   

Project "illumizymes" data sheet

The following table provides information about the project.

Coordinator
JULIUS-MAXIMILIANS-UNIVERSITAT WURZBURG 

Organization address
address: SANDERRING 2
city: WUERZBURG
postcode: 97070
website: http://www.uni-wuerzburg.de

contact info
title: n.a.
name: n.a.
surname: n.a.
function: n.a.
email: n.a.
telephone: n.a.
fax: n.a.

 Coordinator Country Germany [DE]
 Total cost 2˙061˙250 €
 EC max contribution 2˙061˙250 € (100%)
 Programme 1. H2020-EU.1.1. (EXCELLENT SCIENCE - European Research Council (ERC))
 Code Call ERC-2015-CoG
 Funding Scheme ERC-COG
 Starting year 2016
 Duration (year-month-day) from 2016-07-01   to  2021-06-30

 Partnership

Take a look of project's partnership.

# participants  country  role  EC contrib. [€] 
1    JULIUS-MAXIMILIANS-UNIVERSITAT WURZBURG DE (WUERZBURG) coordinator 1˙977˙500.00
2    GEORG-AUGUST-UNIVERSITAT GOTTINGENSTIFTUNG OFFENTLICHEN RECHTS DE (GOTTINGEN) participant 83˙750.00

Map

Leaflet | Map data © OpenStreetMap contributors, CC-BY-SA, Imagery © Mapbox

 Project objective

RNAs are linear biopolymers that consist of only four types of building blocks, but can fold into complex three-dimensional structures that are endowed with selective, high-affinity ligand-binding abilities known as aptamers, and catalytic activities known as ribozymes. Genome projects have brought the surprising insight that a large part of the human genome is transcribed into RNAs, but only a very small fraction is translated into proteins. The actual number of noncoding RNAs with specific functions is currently unknown, but many are considered as prominent regulators of cellular functions. Posttranscriptional modifications of RNA add an extra layer of complexity, but their regulatory roles in RNA metabolism are only poorly understood. Despite the relative simplicity in molecular composition, the available methodological repertoire for manipulation, interrogation and visualization of RNA is rather limited. This project aims to solve the challenge of RNA labeling in both fixed and living cells, using aptamers and ribozymes for RNA imaging and functional characterization. We introduce the term illumizymes for novel tools that attach bio-orthogonal tags and fluorophores to specific RNA sequences, in vitro and in vivo. Ribozymes and aptamers for small, stable, and specific labels will be identified by in vitro selection and systematic evolution of ligands by exponential enrichment (SELEX) to activate the fluorescence of latent chromophores by restricting their conformational flexibility and/or formation of covalent bonds by new bio-orthogonal reactions. Using naturally inspired, cell-permeable and non-toxic ligands, we will specifically select for increased brightness and photostability, and evolve illumizymes into color-switching probes for RNA microscopy. The new genetically encodable RNA devices will find widespread applications in diverse disciplines to enlighten our understanding of cellular RNA functions in health and disease.

 Publications

year authors and title journal last update
List of publications.
2019 Christian Steinmetzger, Irene Bessi, Ann-Kathrin Lenz, Claudia Höbartner
Structure–fluorescence activation relationships of a large Stokes shift fluorogenic RNA aptamer
published pages: 2019, 47, 11538-, ISSN: 0305-1048, DOI: 10.1093/nar/gkz1084
Nucleic Acids Research 2020-01-30
2018 Maksim V. Sednev, Volodymyr Mykhailiuk, Priyanka Choudhury, Julia Halang, Katherine E. Sloan, Markus T. Bohnsack, Claudia Höbartner
N 6 -Methyladenosine-Sensitive RNA-Cleaving Deoxyribozymes
published pages: 15117-15121, ISSN: 1433-7851, DOI: 10.1002/anie.201808745
Angewandte Chemie International Edition 57/46 2019-02-25
2017 Tucker J. Carrocci, Lea Lohe, Matthew J. Ashton, Claudia Höbartner, Aaron A. Hoskins
Debranchase-resistant labeling of RNA using the 10DM24 deoxyribozyme and fluorescent modified nucleotides
published pages: 11992-11995, ISSN: 1359-7345, DOI: 10.1039/c7cc06703h
Chemical Communications 53/88 2019-02-25
2018 Christian Steinmetzger, Navaneethan Palanisamy, Kiran R. Gore, Claudia Höbartner
A Multicolor Large Stokes Shift Fluorogen-Activating RNA Aptamer with Cationic Chromophores
published pages: 5, ISSN: 0947-6539, DOI: 10.1002/chem.201805882
Chemistry - A European Journal weekly 2019-02-25

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