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MuCellWall

Seed mucilage envelope as a new structural model in cell wall research

Total Cost €

0

EC-Contrib. €

0

Partnership

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 Outcomes and results

 MuCellWall project word cloud

Explore the words cloud of the MuCellWall project. It provides you a very rough idea of what is the project "MuCellWall" about.

antibodies    divers    host    fraction    detect    possibilities    hemicelluloses    arabidopsis    dynamic    microstructural    fibrillar    divided    kiel    treatment    artemisia    microscopes    loose    biochemical    pectins    lepidium    exactly    sem    despite    cells    special    organization    polysaccharides    arranged    opening    modern    sativum    restricted    place    seeds    enzymes    location    material    direct    usually    give    mostly    destroy    incomplete    visualize    university    mscs    mutants    complexes    quite    components    msc    leucodens    representative    invasive    gold    interdisciplinary    laboratory    wall    excellent    structural    mucilage    thaliana    created    particles    chemicals    glycoproteins    plant    precise    model    examined    cellulosic    overcome    cell    wild    realistic    attached    secreting    structure    models    visualization    techniques    spatial    extracellular    lcsm    creation    genetic    matrix    network    extensive    combinations   

Project "MuCellWall" data sheet

The following table provides information about the project.

Coordinator		 CHRISTIAN-ALBRECHTS-UNIVERSITAET ZU KIEL 

Organization address
address: OLSHAUSENSTRASSE 40
city: KIEL
postcode: 24118
website: http://www.uni-kiel.de

contact info
title: n.a.
name: n.a.
surname: n.a.
function: n.a.
email: n.a.
telephone: n.a.
fax: n.a.
 Coordinator Country Germany [DE]
 Total cost 171˙460 €
 EC max contribution 171˙460 € (100%)
 Programme 1. H2020-EU.1.3.2. (Nurturing excellence by means of cross-border and cross-sector mobility)
 Code Call H2020-MSCA-IF-2015
 Funding Scheme MSCA-IF-EF-ST
 Starting year 2016
 Duration (year-month-day) from 2016-09-01   to  2018-08-31

 Partnership

Take a look of project's partnership.

# participants  country  role  EC contrib. [€] 
1    CHRISTIAN-ALBRECHTS-UNIVERSITAET ZU KIEL DE (KIEL) coordinator 171˙460.00

Map

 Project objective

The plant cell wall is dynamic, complexes structure composed of polysaccharides divided into fibrillar (cellulosic) fraction and extracellular matrix (hemicelluloses and pectins). All the components are arranged in a network structure characteristic of the cell wall. The proposed, general spatial models of the cell wall based mostly on various biochemical and/or microstructural analysis, which have usually been invasive by treatment with enzymes or chemicals, which can destroy the cell wall organization and allow to visualize a selected components. Despite of extensive research our knowledge about the cell wall is still incomplete and restricted mostly to the biochemical or genetic studies. The main aim of this project will be the determination of the cell wall components i.e. polysaccharides, glycoproteins, and the determination their spatial distribution in a special type of cell wall of the mucilage secreting cells (MSCs). The obtained results will allow creation a direct spatial cell wall model. The used material e.g. seeds of Arabidopsis thaliana mutants and wild type, Artemisia leucodens or Lepidium sativum will be an excellent and so far not examined study model due to characteristic loose spatial structure of the MSC cell wall. The use of modern techniques (in a quite new combinations) e.g. antibodies with attached gold particles and precise visualization in divers microscopes (e.g. SEM, LCSM) available at the host laboratory (Kiel University) will allow to overcome invasive procedures and let to detect the exactly location of the components. The realistic, representative cell wall spatial model will be created allowing for better understanding the different processes taking place within the cell wall. The project will be implemented at an interdisciplinary level opening a new possibilities for various cell wall studies. The project will give a new insight into the cell wall structural studies and lead to significant advances in this field.

 Publications

year authors and title journal last update
List of publications.
2017 Agnieszka Kreitschitz, Stanislav N. Gorb
How does the cell wall ‘stick’ in the mucilage? A detailed microstructural analysis of the seed coat mucilaginous cell wall
published pages: 9-22, ISSN: 0367-2530, DOI: 10.1016/j.flora.2017.02.010 		Flora 229 2019-04-14

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