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CMDNAUP SIGNED

Composition and Mechanism of the DNA-uptake Pilus of Vibrio cholerae

Total Cost €

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EC-Contrib. €

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Partnership

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 CMDNAUP project word cloud

Explore the words cloud of the CMDNAUP project. It provides you a very rough idea of what is the project "CMDNAUP" about.

pathogens    sophisticated    pandemic    cell    human    roles    assembly    polymeric    subtilis    nanomachine    maximum    infrastructures    implying    gram    fellow    disease    action    notably    underlying    blokesch    play    visualise    zooplankton    biological    functions    dna    prospective    combine    outer    mechanisms    antibiotic    vibrio    conserved    works    chance    environment    determined    positive    hgt    answer    cholera    tfp    membrane    bacterial    resistance    naturally    encoding    models    initiates    bacterium    components    rapid    visualised    bacteria    motility    molecular    failed    epfl    pioneered    attachment    actually    bacillus    cholerae    evolution    pathogen    genetic    negative    nothing    mainly    surface    chitinous    extensions    spread    composition    competence    horizontal    transfer    competent    mode    exoskeletons    follow    pilus    training    purified    aquatic    form    causes    had    pili    elucidate    ubiquitous    innovative    minimal    almost    genes    proteins    mechanism    lab    question    gene    free    virulence    machinery    natural    forms   

Project "CMDNAUP" data sheet

The following table provides information about the project.

Coordinator
ECOLE POLYTECHNIQUE FEDERALE DE LAUSANNE 

Organization address
address: BATIMENT CE 3316 STATION 1
city: LAUSANNE
postcode: 1015
website: www.epfl.ch

contact info
title: n.a.
name: n.a.
surname: n.a.
function: n.a.
email: n.a.
telephone: n.a.
fax: n.a.

 Coordinator Country Switzerland [CH]
 Project website https://blokesch-lab.epfl.ch
 Total cost 175˙419 €
 EC max contribution 175˙419 € (100%)
 Programme 1. H2020-EU.1.3.2. (Nurturing excellence by means of cross-border and cross-sector mobility)
 Code Call H2020-MSCA-IF-2015
 Funding Scheme MSCA-IF-EF-ST
 Starting year 2016
 Duration (year-month-day) from 2016-09-01   to  2018-08-31

 Partnership

Take a look of project's partnership.

# participants  country  role  EC contrib. [€] 
1    ECOLE POLYTECHNIQUE FEDERALE DE LAUSANNE CH (LAUSANNE) coordinator 175˙419.00

Map

 Project objective

Horizontal gene transfer (HGT) allows rapid bacterial evolution including the spread of genes encoding antibiotic resistance and virulence factors. The Gram-negative bacterium Vibrio cholerae is an important human pathogen that causes the pandemic disease Cholera. In its natural aquatic environment growth on the chitinous exoskeletons of zooplankton initiates the development of ‘Natural Competence’, a widespread and key form of HGT that allows bacteria to take up free DNA from the environment. DNA uptake involves a sophisticated nanomachine known as a Type IV Pilus (TFP), which forms polymeric extensions from the cell surface, are ubiquitous throughout bacteria and play a wide range of other roles such as surface motility and attachment. Notably, this machinery is conserved in other naturally competent bacteria including in several important human pathogens, implying a common mode of action. Work on this machinery has mainly been done in Gram-positive models like Bacillus subtilis but had failed to visualise an uptake pilus. Recently, the Blokesch lab visualised a DNA uptake pilus extending from the outer membrane of V. cholerae and determined the minimal known components needed for its assembly. However, we still know almost nothing about how this machinery actually works to bring DNA into the cell. To answer this important question and elucidate the underlying molecular mechanisms we will follow two main objectives. 1. We will determine the composition of purified DNA-uptake pili and investigate the functions of the identified proteins. 2. We will combine innovative genetic and cell-biological approaches to investigate the mechanism of DNA uptake. The Blokesch lab at EPFL has pioneered genetic and cell biological methods for studying competence in V. cholerae and has state-of-the-art equipment and infrastructures that offer the prospective fellow the maximum chance of success and the best-possible training through the research.

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The information about "CMDNAUP" are provided by the European Opendata Portal: CORDIS opendata.

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