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EndoSubvert SIGNED

Common mechanisms of host membrane trafficking subversion by intracellular pathogens to rupture bacterial containing vacuoles

Total Cost €

0

EC-Contrib. €

0

Partnership

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 EndoSubvert project word cloud

Explore the words cloud of the EndoSubvert project. It provides you a very rough idea of what is the project "EndoSubvert" about.

clem    cells    compartments    depending    analyze    bacterial    remodeling    cell    fluorescence    definition    monocytogenes    track    trafficking    localization    pathogens    strategy    intracellular    regulation    delineate    antimicrobial    containing    integrity    bcv    model    active    differential    cytoplasmic    innovative    despite    starting    correlative    listeria    bacteria    biological    vacuole    shigella    precise    sensing    cytoplasm    cascades    pathophysiological    leads    tuberculosis    invasion    therapeutic    localize    chooses    scaffolds    complexity    escape    rupture    lacks    discovery    strategies    workflow    hence    salmonella    subversion    fundamental    uncovered    pathogen    microscopy    passive    volume    pathogenic    beam    rational    types    light    scanning    membrane    molecular    induction    understudied    host    electron    epithelial    rab    mechanisms    yield    flexneri    ion    always    mycobacterium    classified    enterica    bound    challenged   

Project "EndoSubvert" data sheet

The following table provides information about the project.

Coordinator
INSTITUT PASTEUR 

Organization address
address: RUE DU DOCTEUR ROUX 25-28
city: PARIS CEDEX 15
postcode: 75724
website: http://www.pasteur.fr

contact info
title: n.a.
name: n.a.
surname: n.a.
function: n.a.
email: n.a.
telephone: n.a.
fax: n.a.

 Coordinator Country France [FR]
 Total cost 2˙000˙000 €
 EC max contribution 2˙000˙000 € (100%)
 Programme 1. H2020-EU.1.1. (EXCELLENT SCIENCE - European Research Council (ERC))
 Code Call ERC-2015-CoG
 Funding Scheme ERC-COG
 Starting year 2017
 Duration (year-month-day) from 2017-01-01   to  2021-12-31

 Partnership

Take a look of project's partnership.

# participants  country  role  EC contrib. [€] 
1    INSTITUT PASTEUR FR (PARIS CEDEX 15) coordinator 2˙000˙000.00

Map

 Project objective

A common strategy of bacterial pathogens is active or passive uptake into host cells. There, they can localize within a bacterial containing vacuole (BCV) or access the host cytoplasm through BCV rupture. Hence, intracellular pathogens are often classified as vacuole-bound or cytoplasmic. Recently, this definition has been challenged by the discovery that many vacuole-bound pathogens, including Mycobacterium tuberculosis and Salmonella enterica, access the host cytoplasm, and by the insight that cytoplasmic bacteria, like Shigella flexneri or Listeria monocytogenes, do not always escape the BCV. Despite this increasing complexity, a precise understanding lacks for why and how a pathogen “chooses” between a BCV or the cytoplasm and yet this is very important: because of differential pathogen sensing in membrane-bound and cytoplasmic compartments, intracellular localization leads to induction of different host responses. Therefore, a comprehensive understanding of the processes controlling BCV integrity is not only essential, but can provide new therapeutic targets. Our previous research has implemented innovative fluorescence microscopy to track the invasion steps of pathogenic bacteria. We have further integrated a large-volume, correlative, light/electron microscopy (CLEM) workflow via focused ion beam scanning electron microscopy. This uncovered the subversion of host Rab cascades by Shigella to rupture its BCV. Starting with the Shigella model of epithelial cell invasion, we will delineate the precise molecular mechanisms controlling BCV integrity in different host cell types. We will analyze (i) the scaffolds of host pathways for membrane remodeling, (ii) their subversion by various pathogens, and (iii) their differential regulation depending on pathophysiological conditions. Together, this will allow development of novel, rational antimicrobial strategies and will yield fundamental insight into understudied cell biological mechanisms of membrane trafficking.

 Publications

year authors and title journal last update
List of publications.
2018 Jakub Voznica, Christophe Gardella, Ilia Belotserkovsky, Alexandre Dufour, Jost Enninga, Virginie Stévenin
Identification of Parameters of Host Cell Vulnerability during Salmonella Infection by Quantitative Image Analysis and Modeling
published pages: , ISSN: 0019-9567, DOI: 10.1128/iai.00644-17
Infection and Immunity 86/1 2019-11-07
2017 Chun Hui Sun, Benjamin Wacquier, Daniel I Aguilar, Nathalie Carayol, Kevin Denis, Sylviane Boucherie, Cesar Valencia‐Gallardo, Ceren Simsek, Christophe Erneux, Alexandre Lehman, Jost Enninga, Laurence Arbibe, Philippe Sansonetti, Geneviève Dupont, Laurent Combettes, Guy Tran Van Nhieu
The Shigella type III effector IpgD recodes Ca 2+ signals during invasion of epithelial cells
published pages: 2567-2580, ISSN: 0261-4189, DOI: 10.15252/embj.201696272
The EMBO Journal 36/17 2019-11-07
2018 Jakub Voznica, Jost Enninga, Virginie Stévenin
High-throughput Microscopic Analysis of Salmonella Invasion of Host Cells
published pages: , ISSN: 2331-8325, DOI: 10.21769/bioprotoc.3017
BIO-PROTOCOL 8/18 2019-11-07
2019 Allon Weiner, Jost Enninga
The Pathogen–Host Interface in Three Dimensions: Correlative FIB/SEM Applications
published pages: 426-439, ISSN: 0966-842X, DOI: 10.1016/j.tim.2018.11.011
Trends in Microbiology 27/5 2019-11-07
2016 Magdalena Gil, Jost Enninga
Tracing a fat or sweet lifestyle - New insights on catabolic paths of intracellular Salmonella
published pages: 655-657, ISSN: 2150-5594, DOI: 10.1080/21505594.2017.1283466
Virulence 8/6 2019-11-07
2018 Marine Oberkampf, Camille Guillerey, Juliette Mouriès, Pierre Rosenbaum, Catherine Fayolle, Alexandre Bobard, Ariel Savina, Eric Ogier-Denis, Jost Enninga, Sebastian Amigorena, Claude Leclerc, Gilles Dadaglio
Mitochondrial reactive oxygen species regulate the induction of CD8+ T cells by plasmacytoid dendritic cells
published pages: , ISSN: 2041-1723, DOI: 10.1038/s41467-018-04686-8
Nature Communications 9/1 2019-11-07
2018 Jennifer Fredlund, José Carlos Santos, Virginie Stévenin, Allon Weiner, Patricia Latour-Lambert, Katya Rechav, Adeline Mallet, Jacomine Krijnse-Locker, Michael Elbaum, Jost Enninga
The entry of Salmonella in a distinct tight compartment revealed at high temporal and ultrastructural resolution
published pages: e12816, ISSN: 1462-5814, DOI: 10.1111/cmi.12816
Cellular Microbiology 20/4 2019-11-07
2017 Sonja Kühn, Noelia Lopez-Montero, Yuen-Yan Chang, Anna Sartori-Rupp, Jost Enninga
Imaging macropinosomes during Shigella infections
published pages: 12-22, ISSN: 1046-2023, DOI: 10.1016/j.ymeth.2017.05.007
Methods 127 2019-11-07

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