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EndoSubvert SIGNED

Common mechanisms of host membrane trafficking subversion by intracellular pathogens to rupture bacterial containing vacuoles

Total Cost €

0

EC-Contrib. €

0

Partnership

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 EndoSubvert project word cloud

Explore the words cloud of the EndoSubvert project. It provides you a very rough idea of what is the project "EndoSubvert" about.

analyze    clem    leads    listeria    starting    innovative    containing    strategies    discovery    understudied    pathogen    pathogens    volume    bcv    scaffolds    precise    integrity    host    yield    light    biological    mycobacterium    membrane    workflow    compartments    pathophysiological    localization    invasion    pathogenic    intracellular    lacks    vacuole    induction    bacteria    types    electron    rupture    depending    challenged    flexneri    chooses    cytoplasmic    fundamental    passive    regulation    differential    always    molecular    bound    scanning    ion    complexity    enterica    remodeling    despite    hence    trafficking    cells    rab    active    strategy    uncovered    tuberculosis    cell    model    localize    mechanisms    escape    track    cascades    microscopy    cytoplasm    beam    delineate    shigella    classified    definition    sensing    therapeutic    subversion    bacterial    salmonella    epithelial    monocytogenes    correlative    fluorescence    rational    antimicrobial   

Project "EndoSubvert" data sheet

The following table provides information about the project.

Coordinator
INSTITUT PASTEUR 

Organization address
address: RUE DU DOCTEUR ROUX 25-28
city: PARIS CEDEX 15
postcode: 75724
website: http://www.pasteur.fr

contact info
title: n.a.
name: n.a.
surname: n.a.
function: n.a.
email: n.a.
telephone: n.a.
fax: n.a.

 Coordinator Country France [FR]
 Total cost 2˙000˙000 €
 EC max contribution 2˙000˙000 € (100%)
 Programme 1. H2020-EU.1.1. (EXCELLENT SCIENCE - European Research Council (ERC))
 Code Call ERC-2015-CoG
 Funding Scheme ERC-COG
 Starting year 2017
 Duration (year-month-day) from 2017-01-01   to  2021-12-31

 Partnership

Take a look of project's partnership.

# participants  country  role  EC contrib. [€] 
1    INSTITUT PASTEUR FR (PARIS CEDEX 15) coordinator 2˙000˙000.00

Map

 Project objective

A common strategy of bacterial pathogens is active or passive uptake into host cells. There, they can localize within a bacterial containing vacuole (BCV) or access the host cytoplasm through BCV rupture. Hence, intracellular pathogens are often classified as vacuole-bound or cytoplasmic. Recently, this definition has been challenged by the discovery that many vacuole-bound pathogens, including Mycobacterium tuberculosis and Salmonella enterica, access the host cytoplasm, and by the insight that cytoplasmic bacteria, like Shigella flexneri or Listeria monocytogenes, do not always escape the BCV. Despite this increasing complexity, a precise understanding lacks for why and how a pathogen “chooses” between a BCV or the cytoplasm and yet this is very important: because of differential pathogen sensing in membrane-bound and cytoplasmic compartments, intracellular localization leads to induction of different host responses. Therefore, a comprehensive understanding of the processes controlling BCV integrity is not only essential, but can provide new therapeutic targets. Our previous research has implemented innovative fluorescence microscopy to track the invasion steps of pathogenic bacteria. We have further integrated a large-volume, correlative, light/electron microscopy (CLEM) workflow via focused ion beam scanning electron microscopy. This uncovered the subversion of host Rab cascades by Shigella to rupture its BCV. Starting with the Shigella model of epithelial cell invasion, we will delineate the precise molecular mechanisms controlling BCV integrity in different host cell types. We will analyze (i) the scaffolds of host pathways for membrane remodeling, (ii) their subversion by various pathogens, and (iii) their differential regulation depending on pathophysiological conditions. Together, this will allow development of novel, rational antimicrobial strategies and will yield fundamental insight into understudied cell biological mechanisms of membrane trafficking.

 Publications

year authors and title journal last update
List of publications.
2018 Jakub Voznica, Christophe Gardella, Ilia Belotserkovsky, Alexandre Dufour, Jost Enninga, Virginie Stévenin
Identification of Parameters of Host Cell Vulnerability during Salmonella Infection by Quantitative Image Analysis and Modeling
published pages: , ISSN: 0019-9567, DOI: 10.1128/iai.00644-17
Infection and Immunity 86/1 2019-11-07
2017 Chun Hui Sun, Benjamin Wacquier, Daniel I Aguilar, Nathalie Carayol, Kevin Denis, Sylviane Boucherie, Cesar Valencia‐Gallardo, Ceren Simsek, Christophe Erneux, Alexandre Lehman, Jost Enninga, Laurence Arbibe, Philippe Sansonetti, Geneviève Dupont, Laurent Combettes, Guy Tran Van Nhieu
The Shigella type III effector IpgD recodes Ca 2+ signals during invasion of epithelial cells
published pages: 2567-2580, ISSN: 0261-4189, DOI: 10.15252/embj.201696272
The EMBO Journal 36/17 2019-11-07
2018 Jakub Voznica, Jost Enninga, Virginie Stévenin
High-throughput Microscopic Analysis of Salmonella Invasion of Host Cells
published pages: , ISSN: 2331-8325, DOI: 10.21769/bioprotoc.3017
BIO-PROTOCOL 8/18 2019-11-07
2019 Allon Weiner, Jost Enninga
The Pathogen–Host Interface in Three Dimensions: Correlative FIB/SEM Applications
published pages: 426-439, ISSN: 0966-842X, DOI: 10.1016/j.tim.2018.11.011
Trends in Microbiology 27/5 2019-11-07
2016 Magdalena Gil, Jost Enninga
Tracing a fat or sweet lifestyle - New insights on catabolic paths of intracellular Salmonella
published pages: 655-657, ISSN: 2150-5594, DOI: 10.1080/21505594.2017.1283466
Virulence 8/6 2019-11-07
2018 Marine Oberkampf, Camille Guillerey, Juliette Mouriès, Pierre Rosenbaum, Catherine Fayolle, Alexandre Bobard, Ariel Savina, Eric Ogier-Denis, Jost Enninga, Sebastian Amigorena, Claude Leclerc, Gilles Dadaglio
Mitochondrial reactive oxygen species regulate the induction of CD8+ T cells by plasmacytoid dendritic cells
published pages: , ISSN: 2041-1723, DOI: 10.1038/s41467-018-04686-8
Nature Communications 9/1 2019-11-07
2018 Jennifer Fredlund, José Carlos Santos, Virginie Stévenin, Allon Weiner, Patricia Latour-Lambert, Katya Rechav, Adeline Mallet, Jacomine Krijnse-Locker, Michael Elbaum, Jost Enninga
The entry of Salmonella in a distinct tight compartment revealed at high temporal and ultrastructural resolution
published pages: e12816, ISSN: 1462-5814, DOI: 10.1111/cmi.12816
Cellular Microbiology 20/4 2019-11-07
2017 Sonja Kühn, Noelia Lopez-Montero, Yuen-Yan Chang, Anna Sartori-Rupp, Jost Enninga
Imaging macropinosomes during Shigella infections
published pages: 12-22, ISSN: 1046-2023, DOI: 10.1016/j.ymeth.2017.05.007
Methods 127 2019-11-07

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