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Nod2Root SIGNED

Keeping nodules in check: Interplay of rhizobial and host factors controlling nodule morphogenesis and identity in legume plants.

Total Cost €

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EC-Contrib. €

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Partnership

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 Nod2Root project word cloud

Explore the words cloud of the Nod2Root project. It provides you a very rough idea of what is the project "Nod2Root" about.

bacteria    former    reporter    bradyrhizobium    roots    genetics    metabolic    stress    nodule    nodulation    metabolomics    biology    morphogenetic    soybean    pointing    determinate    economically    persistence    altogether    metabolites    nodules    cellular    insights    light    plants    takes    ecfg    interestingly    wp2    diazoefficiens    genes    regulation    medicago    transcriptomics    truncatula    specialized    cells    underlying    organs    found    hosts    molecular    organogenesis    signalling    sigma    decipher    root    combination    mutants    rhizobial    fixation    identity    cell    model    elicited    plant    wp1    fusions    attempts    recognition    orthologs    unravelled    formed    repressing    group    legumes    pisum    mutant    unravel    conversion    determinants    integrity    gene    place    legume    mechanisms    wp3    networks    origin    deep    symbiotic    delta    nitrogen    maintained    checkpoint    ongoing    ectopic    tip    bop    host    nbcl    sativum    noot    lacking    combine    rhizobia    coch    engineer   

Project "Nod2Root" data sheet

The following table provides information about the project.

Coordinator
EIDGENOESSISCHE TECHNISCHE HOCHSCHULE ZUERICH 

Organization address
address: Raemistrasse 101
city: ZUERICH
postcode: 8092
website: https://www.ethz.ch/de.html

contact info
title: n.a.
name: n.a.
surname: n.a.
function: n.a.
email: n.a.
telephone: n.a.
fax: n.a.

 Coordinator Country Switzerland [CH]
 Project website http://www.micro.biol.ethz.ch/research/vorholt/fischer.html
 Total cost 187˙419 €
 EC max contribution 187˙419 € (100%)
 Programme 1. H2020-EU.1.3.2. (Nurturing excellence by means of cross-border and cross-sector mobility)
 Code Call H2020-MSCA-IF-2016
 Funding Scheme MSCA-IF-EF-ST
 Starting year 2017
 Duration (year-month-day) from 2017-03-01   to  2019-02-28

 Partnership

Take a look of project's partnership.

# participants  country  role  EC contrib. [€] 
1    EIDGENOESSISCHE TECHNISCHE HOCHSCHULE ZUERICH CH (ZUERICH) coordinator 187˙419.00

Map

 Project objective

Symbiotic nitrogen fixation by rhizobia takes place in specialized organs of legume host plants, the root nodules. While rather deep molecular insights into plant-rhizobia recognition, early nodule organogenesis, regulation of nodulation and nitrogen fixation are available, much less is known about how nodule integrity is maintained and the origin of the underlying morphogenetic program. Recently, NOOT BOP COCH LIKE (NBCL) genes of Medicago truncatula and Pisum sativum were found to ensure nodule integrity by repressing ectopic root formation (applicant's former project). Interestingly, soybean nodules elicited by a Bradyrhizobium diazoefficiens mutant lacking the general stress response sigma factor σEcfG also formed ectopic roots (host's project), pointing to a bacteria-plant signalling system that is crucial for nodule persistence and integrity. Here, we propose to decipher the molecular determinants that maintain determinate nodule identity using the B. diazoefficiens – soybean model. We will combine plant molecular genetics, cell biology with transcriptomics and metabolomics to unravel cells, genes and metabolic networks that contribute to the checkpoint system ensuring nodule integrity. In work package 1 (WP1), the role of three soybean NBCL orthologs will be unravelled. In WP2, the nodule-to-root conversion characteristic for B. diazoefficiens ΔecfG mutants will be studied at the cellular level using root tip-specific reporter fusions. In WP3, a combination of metabolomics and transcriptomics will be applied to identify metabolites and gene networks involved in the nodule-to-root conversion elicited by ΔecfG and nbcl mutants. Altogether, results of this project will provide insight into determinants and mechanisms used by the economically important group of soybean plants to maintain the integrity of specialized, root-derived organs, an aspect that is relevant also in the light of ongoing attempts to engineer non-legumes into rhizobial hosts.

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