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MELBA

MEMBRANE PROTEINS OF ELECTROACTIVE BACTERIA PROBED AT LIPID LAYERS ONTO MODIFIED ELECTRODES

Total Cost €

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EC-Contrib. €

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Partnership

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 Outcomes and results

 MELBA project word cloud

Explore the words cloud of the MELBA project. It provides you a very rough idea of what is the project "MELBA" about.

researcher    incorporated    profile    eacute    negative    science    universidade    electrochemistry    technologies    extraction    layers    gram    bacteria    ph    communications    expertise    modified    probing    melba    solid    international    charge    lipid    versatile    bioelectrochemistry    electron    ur1    acquire    lisboa    outcome    de    grants    carbon    efficient    electroactive    france    protein    academic    positions    wp2    expression    oral    unl    understand    deposited    permanent    purification    create    universit    extracellular    months    optimize    six    host    nova    protonic    transfer    responsive    leb    covalently    task2    electrochemical    task3    materials    interdisciplinary    gue    artificial    wp1    modification    enter    formal    proteins    microbiology    public    egrave    task1    compete    biochemistry    characterization    ambition    transport    scheduled    membrane    published    surface    conferences    secondment    rennes    proton    techniques    electrophores    extend    extracted    onto    electronic    electrodes    coupling    task4    dr    portugal    papers    actions    platform    engagement    microbial   

Project "MELBA" data sheet

The following table provides information about the project.

Coordinator		 UNIVERSITE DE RENNES I 

Organization address
address: RUE DU THABOR 2
city: RENNES CEDEX
postcode: 35065
website: www.univ-rennes1.fr

contact info
title: n.a.
name: n.a.
surname: n.a.
function: n.a.
email: n.a.
telephone: n.a.
fax: n.a.
 Coordinator Country France [FR]
 Project website https://www.univ-rennes1.fr/actualites/03102017/concevoir-une-plateforme-electrochimique-polyvalente-pour-sonder-les-proteines
 Total cost 185˙076 €
 EC max contribution 185˙076 € (100%)
 Programme 1. H2020-EU.1.3.2. (Nurturing excellence by means of cross-border and cross-sector mobility)
 Code Call H2020-MSCA-IF-2016
 Funding Scheme MSCA-IF-EF-ST
 Starting year 2017
 Duration (year-month-day) from 2017-04-01   to  2019-07-22

 Partnership

Take a look of project's partnership.

# participants  country  role  EC contrib. [€] 
1    UNIVERSITE DE RENNES I FR (RENNES CEDEX) coordinator 185˙076.00

Map

 Project objective

MELBA aims at developing an efficient and versatile electrochemical platform for probing the charge transfer properties, electronic or protonic, of membrane proteins extracted from Gram negative electroactive bacteria. This 2 years research project will be conducted at the Université de Rennes 1, UR1 France, including a six months Secondment at Universidade Nova de Lisboa, UNL Portugal. Carbon electrodes will be covalently modified with pH-responsive electrophores through surface modification techniques, WP1 Task1. Lipid layers will be deposited onto the modified electrodes to create an artificial membrane as a host for extracted membrane proteins, WP1 Task2. The experienced researcher Dr. Lebègue will learn protein expression, extraction, purification and characterization during the six months Secondment at UNL, WP2 Task3. Finally extracted proteins will be incorporated into the artificial membrane deposited onto the modified electrodes and their electronic and/or protonic charge transfer properties will be characterized, WP2 Task4. The ambition of MELBA is to understand the coupling of extracellular electron transfer with proton transport in Gram (-) electroactive bacteria. The outcome of MELBA will be useful to optimize potential microbial electrochemical technologies based on electroactive bacteria and will be applied to the characterization of the charge transfer properties of any membrane protein. Within MELBA, 3 oral communications will be given at international conferences, 5 high impact papers will be published in the field of bioelectrochemistry and 5 formal public engagement actions have been scheduled. MELBA will allow Dr. Lebègue to develop and extend her expertise in electrochemistry, materials science and surface modification to microbiology and biochemistry. Dr. Lebègue will enter the field of microbial electrochemistry and acquire a unique and solid interdisciplinary profile, required to compete for permanent academic positions and grants in Europe.

 Publications

year authors and title journal last update
List of publications.
2019 Estelle Lebègue, Nazua L. Costa, Bruno M. Fonseca, Ricardo O. Louro, Frédéric Barrière
Electrochemical properties of pH-dependent flavocytochrome c3 from Shewanella putrefaciens adsorbed onto unmodified and catechol-modified edge plane pyrolytic graphite electrode
published pages: 113232, ISSN: 1572-6657, DOI: 10.1016/j.jelechem.2019.113232 		Journal of Electroanalytical Chemistry 847 2019-11-13
2018 Estelle Lebègue, Hassiba Smida, Thomas Flinois, Véronique Vié, Corinne Lagrost, Frédéric Barrière
An optimal surface concentration of pure cardiolipin deposited onto glassy carbon electrode promoting the direct electron transfer of cytochrome- c
published pages: 286-292, ISSN: 1572-6657, DOI: 10.1016/j.jelechem.2017.12.024 		Journal of Electroanalytical Chemistry 808 2019-11-13
2018 Hassiba Smida, Estelle Lebègue, Jean-François Bergamini, Frédéric Barrière, Corinne Lagrost
Reductive electrografting of in situ produced diazopyridinium cations: Tailoring the interface between carbon electrodes and electroactive bacterial films
published pages: 157-165, ISSN: 1567-5394, DOI: 10.1016/j.bioelechem.2017.12.006 		Bioelectrochemistry 120 2019-11-13
2018 Estelle Lebègue, Ricardo O. Louro, Frédéric Barrière
Electrochemical Detection of pH-Responsive Grafted Catechol and Immobilized Cytochrome c onto Lipid Deposit-Modified Glassy Carbon Surface
published pages: 9035-9042, ISSN: 2470-1343, DOI: 10.1021/acsomega.8b01425 		ACS Omega 3/8 2019-11-13

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