EU H2020 Project "MELBA (MEMBRANE PROTEINS OF ELECTROACTIVE BACTERIA PROBED AT LIPID LAYERS ONTO..)": description, partecipants, costs and EC-fundings

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MELBA project word cloud

Explore the words cloud of the MELBA project. It provides you a very rough idea of what is the project "MELBA" about.

gue membrane characterization rennes leb task3 extraction techniques outcome incorporated international electrophores materials profile months onto platform published deposited bacteria negative gram ph optimize engagement technologies electrochemical understand acquire charge purification transport wp1 electrochemistry coupling layers task1 public researcher protonic solid papers versatile wp2 electronic electrodes host proton nova surface conferences ur1 create artificial compete electroactive oral scheduled interdisciplinary transfer france science biochemistry secondment universidade task4 probing communications modified extracted protein de bioelectrochemistry actions egrave carbon expertise extend portugal expression six permanent microbiology positions ambition academic proteins covalently efficient task2 microbial dr unl extracellular universit lisboa lipid enter grants modification eacute formal electron responsive melba

Project "MELBA" data sheet

The following table provides information about the project.

Coordinator	UNIVERSITE DE RENNES I Organization address address: RUE DU THABOR 2 city: RENNES CEDEX postcode: 35065 website: www.univ-rennes1.fr contact info title: n.a. name: n.a. surname: n.a. function: n.a. email: n.a. telephone: n.a. fax: n.a.
Coordinator Country	France [FR]
Project website	https://www.univ-rennes1.fr/actualites/03102017/concevoir-une-plateforme-electrochimique-polyvalente-pour-sonder-les-proteines
Total cost	185˙076 €
EC max contribution	185˙076 € (100%)
Programme	1. H2020-EU.1.3.2. (Nurturing excellence by means of cross-border and cross-sector mobility)
Code Call	H2020-MSCA-IF-2016
Funding Scheme	MSCA-IF-EF-ST
Starting year	2017
Duration (year-month-day)	from 2017-04-01 to 2019-07-22

#	participants	country	role	EC contrib. [€]
1	UNIVERSITE DE RENNES I	FR (RENNES CEDEX)	coordinator	185˙076.00

UNIVERSITE DE RENNES I

FR (RENNES CEDEX)

coordinator

185˙076.00

Project objective

MELBA aims at developing an efficient and versatile electrochemical platform for probing the charge transfer properties, electronic or protonic, of membrane proteins extracted from Gram negative electroactive bacteria. This 2 years research project will be conducted at the Université de Rennes 1, UR1 France, including a six months Secondment at Universidade Nova de Lisboa, UNL Portugal. Carbon electrodes will be covalently modified with pH-responsive electrophores through surface modification techniques, WP1 Task1. Lipid layers will be deposited onto the modified electrodes to create an artificial membrane as a host for extracted membrane proteins, WP1 Task2. The experienced researcher Dr. Lebègue will learn protein expression, extraction, purification and characterization during the six months Secondment at UNL, WP2 Task3. Finally extracted proteins will be incorporated into the artificial membrane deposited onto the modified electrodes and their electronic and/or protonic charge transfer properties will be characterized, WP2 Task4. The ambition of MELBA is to understand the coupling of extracellular electron transfer with proton transport in Gram (-) electroactive bacteria. The outcome of MELBA will be useful to optimize potential microbial electrochemical technologies based on electroactive bacteria and will be applied to the characterization of the charge transfer properties of any membrane protein. Within MELBA, 3 oral communications will be given at international conferences, 5 high impact papers will be published in the field of bioelectrochemistry and 5 formal public engagement actions have been scheduled. MELBA will allow Dr. Lebègue to develop and extend her expertise in electrochemistry, materials science and surface modification to microbiology and biochemistry. Dr. Lebègue will enter the field of microbial electrochemistry and acquire a unique and solid interdisciplinary profile, required to compete for permanent academic positions and grants in Europe.

Publications

List of publications.
year	authors and title	journal	last update
2019	Estelle LebÃ¨gue, Nazua L. Costa, Bruno M. Fonseca, Ricardo O. Louro, FrÃ©dÃ©ric BarriÃ¨re Electrochemical properties of pH-dependent flavocytochrome c3 from Shewanella putrefaciens adsorbed onto unmodified and catechol-modified edge plane pyrolytic graphite electrode published pages: 113232, ISSN: 1572-6657, DOI: 10.1016/j.jelechem.2019.113232	Journal of Electroanalytical Chemistry 847	2019-11-13
2018	Estelle LebÃ¨gue, Hassiba Smida, Thomas Flinois, VÃ©ronique ViÃ©, Corinne Lagrost, FrÃ©dÃ©ric BarriÃ¨re An optimal surface concentration of pure cardiolipin deposited onto glassy carbon electrode promoting the direct electron transfer of cytochrome- c published pages: 286-292, ISSN: 1572-6657, DOI: 10.1016/j.jelechem.2017.12.024	Journal of Electroanalytical Chemistry 808	2019-11-13
2018	Hassiba Smida, Estelle LebÃ¨gue, Jean-FranÃ§ois Bergamini, FrÃ©dÃ©ric BarriÃ¨re, Corinne Lagrost Reductive electrografting of in situ produced diazopyridinium cations: Tailoring the interface between carbon electrodes and electroactive bacterial films published pages: 157-165, ISSN: 1567-5394, DOI: 10.1016/j.bioelechem.2017.12.006	Bioelectrochemistry 120	2019-11-13
2018	Estelle LebÃ¨gue, Ricardo O. Louro, FrÃ©dÃ©ric BarriÃ¨re Electrochemical Detection of pH-Responsive Grafted Catechol and Immobilized Cytochrome c onto Lipid Deposit-Modified Glassy Carbon Surface published pages: 9035-9042, ISSN: 2470-1343, DOI: 10.1021/acsomega.8b01425	ACS Omega 3/8	2019-11-13

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