Opendata, web and dolomites


Design and Engineering Next-Generation Nanopore Devices for Bioplymer Analysis

Total Cost €


EC-Contrib. €






Project "DeE-Nano" data sheet

The following table provides information about the project.


Organization address
address: Broerstraat 5
postcode: 9712CP

contact info
title: n.a.
name: n.a.
surname: n.a.
function: n.a.
email: n.a.
telephone: n.a.
fax: n.a.

 Coordinator Country Netherlands [NL]
 Total cost 1˙999˙970 €
 EC max contribution 1˙999˙970 € (100%)
 Programme 1. H2020-EU.1.1. (EXCELLENT SCIENCE - European Research Council (ERC))
 Code Call ERC-2016-COG
 Funding Scheme ERC-COG
 Starting year 2017
 Duration (year-month-day) from 2017-07-01   to  2022-06-30


Take a look of project's partnership.

# participants  country  role  EC contrib. [€] 


 Project objective

Nanopores have emerged in the past few years as a promising analytical technique. The basic concept of nanopore sensing is to apply a potential across individual nanoscale pores and observe the disruption of the ionic flow caused by single molecules entering the pore. Ionic currents through protein pores have been successful at recognizing tiny differences in molecules in solution. Most notably, arrays of thousands of nanopores integrated in low-cost and portable devices are now capable of sequencing DNA at the single-molecule level. The main challenge of nanopore sensing is the inability of controlling the protein pore diameter and geometry, which determines the signal and enables selectivity based on physical size.

The aim of this proposal is to design a new generation of protein nanopores that will take on the next grand challenge in nanopore sensing, that is the sequence identification of single proteins.

In order to sequence proteins, the designed nanopores must: unfold a target protein, control the speed of its transit across the nanopore and recognize individual amino acids. Our approach is to design a transmembrane molecular machine that will unfold target proteins and feed the linearize polypeptide through the nanopore where single amino acids will be recognized by modulations of the nanopore current. The specific objectives are: i) Develop chemical and biotechnological methods to design synthetic protein-based pores ii) To precisely attach the unfolding machine to a nanopore iii) To genetically engineer the nanopore for optimal amino acid recognition

Our nanopore devices will be used to develop the first technology to sequence single proteins. Compared to the state of the art ‘shotgun proteomics’, the nanopore approach will allow long polypeptide reads, recognition of low-abundance proteins, including biomarkers linked to diseases, and real-time monitoring with minimal cost, time and sample preparation.


year authors and title journal last update
List of publications.
2019 Laura Restrepo-Pérez, Chun Heung Wong, Giovanni Maglia, Cees Dekker, Chirlmin Joo
Label-Free Detection of Post-translational Modifications with a Nanopore
published pages: 7957-7964, ISSN: 1530-6984, DOI: 10.1021/acs.nanolett.9b03134
Nano Letters 19/11 2020-04-01

Are you the coordinator (or a participant) of this project? Plaese send me more information about the "DEE-NANO" project.

For instance: the website url (it has not provided by EU-opendata yet), the logo, a more detailed description of the project (in plain text as a rtf file or a word file), some pictures (as picture files, not embedded into any word file), twitter account, linkedin page, etc.

Send me an  email ( and I put them in your project's page as son as possible.

Thanks. And then put a link of this page into your project's website.

The information about "DEE-NANO" are provided by the European Opendata Portal: CORDIS opendata.

More projects from the same programme (H2020-EU.1.1.)

DINAMIX (2019)

Real-time diffusion NMR analysis of mixtures

Read More  

EAST (2020)

Using Evolutionary Algorithms to Understand and Secure Web/Enterprise Systems

Read More  

EVEREST (2020)

Extracellular Vesicles for Bone Regeneration – alternatives to Stem-cell Therapy

Read More