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FASTER SIGNED

Faster magic-angle spinning leads to a resolution revolution in biological solid-state NMR

Total Cost €

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EC-Contrib. €

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Partnership

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 FASTER project word cloud

Explore the words cloud of the FASTER project. It provides you a very rough idea of what is the project "FASTER" about.

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Project "FASTER" data sheet

The following table provides information about the project.

Coordinator
EIDGENOESSISCHE TECHNISCHE HOCHSCHULE ZUERICH 

Organization address
address: Raemistrasse 101
city: ZUERICH
postcode: 8092
website: https://www.ethz.ch/de.html

contact info
title: n.a.
name: n.a.
surname: n.a.
function: n.a.
email: n.a.
telephone: n.a.
fax: n.a.

 Coordinator Country Switzerland [CH]
 Total cost 2˙173˙375 €
 EC max contribution 2˙173˙375 € (100%)
 Programme 1. H2020-EU.1.1. (EXCELLENT SCIENCE - European Research Council (ERC))
 Code Call ERC-2016-ADG
 Funding Scheme ERC-ADG
 Starting year 2017
 Duration (year-month-day) from 2017-10-01   to  2022-09-30

 Partnership

Take a look of project's partnership.

# participants  country  role  EC contrib. [€] 
1    EIDGENOESSISCHE TECHNISCHE HOCHSCHULE ZUERICH CH (ZUERICH) coordinator 2˙173˙375.00

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 Project objective

Solid-state NMR has recently made a significant impact on structural biology by providing atomic-resolution structures of several, previously uncharacterized proteins. A particularly relevant example is the Amyloid-beta (Aβ) peptide linked to Alzheimer’s disease where we determined the atomic-resolution structure of Aβ(1-42) and of the Osaka mutant of Aβ(1-40). A spectral resolution revolution is now in reach that will enable solid-state NMR to address new frontiers in structural biology. The applications mentioned above are based on 13C-detected spectroscopy. Proton-detected experiments, although clearly more sensitive thanks to the high gyromagnetic ratio of 1H, have found few applications so far, due to the poor resolution of 1H spectra caused by the 1H-1H dipolar interaction. The proton resolution can be enhanced by employing faster rotation of the sample, i.e. higher MAS (magic-angle spinning) frequencies. Presently accessible MAS frequencies are already faster than the ones of any other man-made object. A significant improvement is still attainable in our view. Increasing the MAS frequency to 200-250 kHz will improve the spectral quality to favorably compare with solution NMR for larger proteins, including fully protonated systems. In addition, the amount of sample required is reduced by almost two orders of magnitude, to approx. 100 μg, compared to the about 10 mg needed in 13C-detected experiments. This removes an important bottleneck in sample-preparation. The resolution and sensitivity gain will allow the structural characterization of e.g. disease-relevant amyloids or membrane proteins with higher precision. Moreover, this approach will enable the investigation of complex systems, which presently elude structural characterization. The resolution revolution brought about by fast spinning shall thus represent a breakthrough since it will open new horizons for solving urgent biological and medical questions.

 Publications

year authors and title journal last update
List of publications.
2019 Albert A. Smith, Matthias Ernst, Sereina Riniker, Beat H. Meier
Localized and Collective Motions in HET‐s(218‐289) Fibrils from Combined NMR Relaxation and MD Simulation
published pages: 9383-9388, ISSN: 1433-7851, DOI: 10.1002/anie.201901929
Angewandte Chemie International Edition 58/28 2020-04-24
2019 Lauriane Lecoq, Maarten Schledorn, Shishan Wang, Susanne Smith-Penzel, Alexander A. Malär, Morgane Callon, Michael Nassal, Beat H. Meier, Anja Böckmann
100 kHz MAS Proton-Detected NMR Spectroscopy of Hepatitis B Virus Capsids
published pages: , ISSN: 2296-889X, DOI: 10.3389/fmolb.2019.00058
Frontiers in Molecular Biosciences 6 2020-04-24
2019 Susanne Penzel, Andres Oss, Mai-Liis Org, Ago Samoson, Anja Böckmann, Matthias Ernst, Beat H. Meier
Spinning faster: protein NMR at MAS frequencies up to 126 kHz
published pages: 19-29, ISSN: 0925-2738, DOI: 10.1007/s10858-018-0219-9
Journal of Biomolecular NMR 73/1-2 2019-06-11
2018 Daniel Stöppler, Alex Macpherson, Susanne Smith-Penzel, Nicolas Basse, Fabien Lecomte, Hervé Deboves, Richard D. Taylor, Tim Norman, John Porter, Lorna C. Waters, Marta Westwood, Ben Cossins, Katharine Cain, James White, Robert Griffin, Christine Prosser, Sebastian Kelm, Amy H. Sullivan, David Fox, Mark D. Carr, Alistair Henry, Richard Taylor, Beat H. Meier, Hartmut Oschkinat, Alastair D. Lawson
Insight into small molecule binding to the neonatal Fc receptor by X-ray crystallography and 100 kHz magic-angle-spinning NMR
published pages: e2006192, ISSN: 1545-7885, DOI: 10.1371/journal.pbio.2006192
PLOS Biology 16/5 2019-04-26
2018 Albert A. Smith, Matthias Ernst, Beat H. Meier
Optimized “detectors” for dynamics analysis in solid-state NMR
published pages: 45104, ISSN: 0021-9606, DOI: 10.1063/1.5013316
The Journal of Chemical Physics 148/4 2019-04-26
2019 Thomas Wiegand, Riccardo Cadalbert, Denis Lacabanne, Joanna Timmins, Laurent Terradot, Anja Böckmann, Beat H. Meier
The conformational changes coupling ATP hydrolysis and translocation in a bacterial DnaB helicase
published pages: 31, ISSN: 2041-1723, DOI: 10.1038/s41467-018-07968-3
Nature Communications 10/1 2019-04-26

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