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VisTrans SIGNED

Visualising transport dynamics of transmembrane pumps

Total Cost €

0

EC-Contrib. €

0

Partnership

0

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 VisTrans project word cloud

Explore the words cloud of the VisTrans project. It provides you a very rough idea of what is the project "VisTrans" about.

pumps    understand    dissipate    atp    strains    diverse    drive    tripartite    situ    intermediates    visualize    mechanistic    cooperative    assemblies    gram    switch    encompassing    pathogenic    transport    regulation    machines    toxins    assembled    translocation    description    envelope    indicates    cell    virulence    molecular    central    quaternary    hydrolysis    conformational    basis    cycles    structural    energy    look    assembly    energized    series    binding    drug    bacteria    observations    gradients    secondary    stereochemical    secretion    electrochemical    channel    efflux    futile    protein    negative    questions    vivo    crystallography    complemented    threading    isolation    structure    pump    engineer    substrate    switching    cycle    macrolide    co    tertiary    regulated    modulated    antibiotics    peptides    intermediate    earlier    substrates    vitro    transporters    constricted    capture    small    prepare    play    resistance    mechanism    cooperativity    polypeptides    cryoem    translocate    toxin    resolution   

Project "VisTrans" data sheet

The following table provides information about the project.

Coordinator
THE CHANCELLOR MASTERS AND SCHOLARSOF THE UNIVERSITY OF CAMBRIDGE 

Organization address
address: TRINITY LANE THE OLD SCHOOLS
city: CAMBRIDGE
postcode: CB2 1TN
website: www.cam.ac.uk

contact info
title: n.a.
name: n.a.
surname: n.a.
function: n.a.
email: n.a.
telephone: n.a.
fax: n.a.

 Coordinator Country United Kingdom [UK]
 Total cost 2˙208˙618 €
 EC max contribution 2˙208˙618 € (100%)
 Programme 1. H2020-EU.1.1. (EXCELLENT SCIENCE - European Research Council (ERC))
 Code Call ERC-2016-ADG
 Funding Scheme ERC-ADG
 Starting year 2017
 Duration (year-month-day) from 2017-09-01   to  2022-08-31

 Partnership

Take a look of project's partnership.

# participants  country  role  EC contrib. [€] 
1    THE CHANCELLOR MASTERS AND SCHOLARSOF THE UNIVERSITY OF CAMBRIDGE UK (CAMBRIDGE) coordinator 2˙208˙618.00

Map

 Project objective

The project will investigate multi-component molecular machines that drive substrates across the cell envelope of bacteria. Some of the machines pump antibiotics or toxins, and so contribute to drug resistance and virulence in pathogenic strains. Questions that will be addressed include what the molecular pumps look like, how they are assembled and regulated, how they capture and translocate substrates, and the stereochemical basis for the cooperative switching of substrate-binding states. Molecular pumps that will be studied include tripartite systems driven by ATP hydrolysis, which play a central role in the efflux of macrolide antibiotics and secretion of toxins in Gram-negative bacteria, and those that use secondary transporters energized by electrochemical gradients. We will build upon our earlier observations to prepare a series of intermediates encompassing the key steps in the transport processes, to visualize tertiary and quaternary structural changes, the pathway of substrates in the efflux pumps, and the threading of toxin polypeptides through the constricted channel in the secretion assembly. The pumps and secretion systems cycle through intermediate states, and these will be studied at high resolution by cryoEM and crystallography to understand how the conformational states switch with strong cooperativity and avoid futile cycles that dissipate energy. Our work indicates that the activity of these transporters can be modulated by small peptides and potential co-factors, and we will address how these work. The project will build on our novel approach to engineer the pump assemblies that enables structural analysis at high resolution in isolation and in situ, and will be complemented with mechanistic analyses in vitro and in vivo. The project will deliver a comprehensive, structure-based description of the mechanism of drug efflux and protein translocation by transport machines and their regulation in diverse pathogenic bacteria.

 Publications

year authors and title journal last update
List of publications.
2018 Dijun Du, Xuan Wang-Kan, Arthur Neuberger, Hendrik W. van Veen, Klaas M. Pos, Laura J. V. Piddock, Ben F. Luisi
Multidrug efflux pumps: structure, function and regulation
published pages: 523-539, ISSN: 1740-1526, DOI: 10.1038/s41579-018-0048-6
Nature Reviews Microbiology 16/9 2020-01-28

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