Opendata, web and dolomites

VisTrans SIGNED

Visualising transport dynamics of transmembrane pumps

Total Cost €

0

EC-Contrib. €

0

Partnership

0

Views

0

 VisTrans project word cloud

Explore the words cloud of the VisTrans project. It provides you a very rough idea of what is the project "VisTrans" about.

strains    conformational    questions    pathogenic    drug    efflux    cycles    cryoem    intermediate    translocation    pump    cell    central    peptides    futile    structure    cycle    mechanism    resistance    dissipate    vivo    threading    co    description    switching    secretion    complemented    protein    tripartite    atp    situ    understand    intermediates    assemblies    vitro    drive    crystallography    isolation    transporters    encompassing    capture    binding    modulated    electrochemical    pumps    regulated    machines    envelope    visualize    diverse    gradients    secondary    macrolide    indicates    regulation    cooperativity    translocate    look    channel    small    energized    resolution    constricted    assembled    molecular    basis    toxins    energy    play    substrates    switch    polypeptides    toxin    prepare    transport    antibiotics    negative    structural    quaternary    cooperative    tertiary    series    virulence    stereochemical    assembly    substrate    bacteria    earlier    hydrolysis    mechanistic    gram    observations    engineer   

Project "VisTrans" data sheet

The following table provides information about the project.

Coordinator
THE CHANCELLOR MASTERS AND SCHOLARSOF THE UNIVERSITY OF CAMBRIDGE 

Organization address
address: TRINITY LANE THE OLD SCHOOLS
city: CAMBRIDGE
postcode: CB2 1TN
website: www.cam.ac.uk

contact info
title: n.a.
name: n.a.
surname: n.a.
function: n.a.
email: n.a.
telephone: n.a.
fax: n.a.

 Coordinator Country United Kingdom [UK]
 Total cost 2˙208˙618 €
 EC max contribution 2˙208˙618 € (100%)
 Programme 1. H2020-EU.1.1. (EXCELLENT SCIENCE - European Research Council (ERC))
 Code Call ERC-2016-ADG
 Funding Scheme ERC-ADG
 Starting year 2017
 Duration (year-month-day) from 2017-09-01   to  2022-08-31

 Partnership

Take a look of project's partnership.

# participants  country  role  EC contrib. [€] 
1    THE CHANCELLOR MASTERS AND SCHOLARSOF THE UNIVERSITY OF CAMBRIDGE UK (CAMBRIDGE) coordinator 2˙208˙618.00

Map

 Project objective

The project will investigate multi-component molecular machines that drive substrates across the cell envelope of bacteria. Some of the machines pump antibiotics or toxins, and so contribute to drug resistance and virulence in pathogenic strains. Questions that will be addressed include what the molecular pumps look like, how they are assembled and regulated, how they capture and translocate substrates, and the stereochemical basis for the cooperative switching of substrate-binding states. Molecular pumps that will be studied include tripartite systems driven by ATP hydrolysis, which play a central role in the efflux of macrolide antibiotics and secretion of toxins in Gram-negative bacteria, and those that use secondary transporters energized by electrochemical gradients. We will build upon our earlier observations to prepare a series of intermediates encompassing the key steps in the transport processes, to visualize tertiary and quaternary structural changes, the pathway of substrates in the efflux pumps, and the threading of toxin polypeptides through the constricted channel in the secretion assembly. The pumps and secretion systems cycle through intermediate states, and these will be studied at high resolution by cryoEM and crystallography to understand how the conformational states switch with strong cooperativity and avoid futile cycles that dissipate energy. Our work indicates that the activity of these transporters can be modulated by small peptides and potential co-factors, and we will address how these work. The project will build on our novel approach to engineer the pump assemblies that enables structural analysis at high resolution in isolation and in situ, and will be complemented with mechanistic analyses in vitro and in vivo. The project will deliver a comprehensive, structure-based description of the mechanism of drug efflux and protein translocation by transport machines and their regulation in diverse pathogenic bacteria.

 Publications

year authors and title journal last update
List of publications.
2018 Dijun Du, Xuan Wang-Kan, Arthur Neuberger, Hendrik W. van Veen, Klaas M. Pos, Laura J. V. Piddock, Ben F. Luisi
Multidrug efflux pumps: structure, function and regulation
published pages: 523-539, ISSN: 1740-1526, DOI: 10.1038/s41579-018-0048-6
Nature Reviews Microbiology 16/9 2020-01-28

Are you the coordinator (or a participant) of this project? Plaese send me more information about the "VISTRANS" project.

For instance: the website url (it has not provided by EU-opendata yet), the logo, a more detailed description of the project (in plain text as a rtf file or a word file), some pictures (as picture files, not embedded into any word file), twitter account, linkedin page, etc.

Send me an  email (fabio@fabiodisconzi.com) and I put them in your project's page as son as possible.

Thanks. And then put a link of this page into your project's website.

The information about "VISTRANS" are provided by the European Opendata Portal: CORDIS opendata.

More projects from the same programme (H2020-EU.1.1.)

HyperBio (2019)

Vis-NIR Hyperspectral imaging for biomaterial quality control

Read More  

KineTic (2020)

New Reagents for Quantifying the Routing and Kinetics of T-cell Activation

Read More  

SLAMseq (2019)

SLAMseq: Temporal resolution in gene expression profiling across multiple platforms

Read More