Opendata, web and dolomites

MADMpancreas SIGNED

Using the precision mouse genetic tool MADM to elucidate the role of EGFR in directing beta cell differentiation and pancreatic morphogenesis

Total Cost €

0

EC-Contrib. €

0

Partnership

0

Views

0

 MADMpancreas project word cloud

Explore the words cloud of the MADMpancreas project. It provides you a very rough idea of what is the project "MADMpancreas" about.

delamination    precision    genes    fate    directed    augment    egfr    grade    lineage    considerably    constitute    producing    deal    establishing    differential    investigators    found    initiate    putatively    madmouse    behaviors    hpscs    answers    successful    edge    expressing    insulin    remained    activate    host    situ    epithelium    expertise    commitment    single    cell    quantitatively    group    outcomes    ligand    networks    ductal    elusive    concomitantly    betacellulin    bipotent    pps    disruption    culture    markers    mouse    quantitative    differentiation    label    training    morphogenesis    diminishes    cellular    altered    meet    adapt    individual    transcriptional    manipulate    precursors    mosaic    beta    pancreatic    inherent    activation    double    signaling    questions    upregulation    seeking    progenitors    endocrine    cutting    genetic    elucidate    regulated    madm    seemingly    ngn3    difficulty    cells    career    apicobasal    events    stochastic    polarity    hesc    commit    bi    primitive    pancreas    genetics    levels    tool    therapeutic    potent    differences    endocrinogenesis    ask    observing   

Project "MADMpancreas" data sheet

The following table provides information about the project.

Coordinator
KOBENHAVNS UNIVERSITET 

Organization address
address: NORREGADE 10
city: KOBENHAVN
postcode: 1165
website: www.ku.dk

contact info
title: n.a.
name: n.a.
surname: n.a.
function: n.a.
email: n.a.
telephone: n.a.
fax: n.a.

 Coordinator Country Denmark [DK]
 Total cost 207˙312 €
 EC max contribution 207˙312 € (100%)
 Programme 1. H2020-EU.1.3.2. (Nurturing excellence by means of cross-border and cross-sector mobility)
 Code Call H2020-MSCA-IF-2018
 Funding Scheme MSCA-IF-EF-ST
 Starting year 2019
 Duration (year-month-day) from 2019-06-01   to  2021-05-31

 Partnership

Take a look of project's partnership.

# participants  country  role  EC contrib. [€] 
1    KOBENHAVNS UNIVERSITET DK (KOBENHAVN) coordinator 207˙312.00

Map

 Project objective

Insulin producing beta cells are derived from bipotent pancreatic progenitors (bi-PPs) that constitute the primitive ductal epithelium. While we now know a great deal about the transcriptional networks required for endocrinogenesis and commitment to the beta lineage, considerably less is understood about the cellular events that initiate delamination and activate differentiation networks. The host group recently found that ligand-specific Egfr signaling is essential for these processes. Signaling by the highly potent Egfr ligand betacellulin diminishes apicobasal polarity leading to upregulation of Ngn3, delamination, and beta cell differentiation. The overall aim of this proposal is to elucidate the role of Egfr signaling in the beta cell differentiation process. I will ask: Is the seemingly stochastic differentiation of bi-PPs regulated by inherent differential Egfr signaling? Do quantitative differences in this single pathway lead to different cellular outcomes? How does the targeted loss of Egfr in Ngn3-expressing endocrine precursors affect their differentiation? What are the genes regulated by Egfr pathway activation that putatively commit Ngn3 progenitors to a beta cell fate? And how does the disruption of endocrine differentiation affect ductal morphogenesis? Answers to these questions have remained elusive because of the difficulty of observing individual cell behaviors in situ in the developing pancreas. To meet this challenge, I will adapt the cutting edge mouse genetic tool Mosaic Analysis with Double Markers (MADM) to quantitatively manipulate Egfr levels in bi-PPs and to concomitantly label altered cells. The successful implementation of MADMouse will deliver findings of immediate interest to investigators seeking to improve the directed differentiation of therapeutic grade beta cells from hPSCs. I will augment my expertise in precision mouse genetics with training in hESC culture enhancing my career goal of establishing my own research group.

Are you the coordinator (or a participant) of this project? Plaese send me more information about the "MADMPANCREAS" project.

For instance: the website url (it has not provided by EU-opendata yet), the logo, a more detailed description of the project (in plain text as a rtf file or a word file), some pictures (as picture files, not embedded into any word file), twitter account, linkedin page, etc.

Send me an  email (fabio@fabiodisconzi.com) and I put them in your project's page as son as possible.

Thanks. And then put a link of this page into your project's website.

The information about "MADMPANCREAS" are provided by the European Opendata Portal: CORDIS opendata.

More projects from the same programme (H2020-EU.1.3.2.)

MingleIFT (2020)

Multi-color and single-molecule fluorescence imaging of intraflagellar transport in the phasmid chemosensory cilia of C. Elegans

Read More  

EPIC (2019)

Evolution of Planktonic Gastropod Calcification

Read More  

NaWaTL (2020)

Narrative, Writing, and the Teotihuacan Language: Exploring Language History Through Phylogenetics, Epigraphy and Iconography

Read More