Opendata, web and dolomites

PHAGOSCOPY SIGNED

PHAGOSCOPY: Dissecting cell-autonomous immunity with ex vivo electron cryo-microscopy

Total Cost €

0

EC-Contrib. €

0

Partnership

0

Views

0

 PHAGOSCOPY project word cloud

Explore the words cloud of the PHAGOSCOPY project. It provides you a very rough idea of what is the project "PHAGOSCOPY" about.

microscopy    killing    microbial    vivo    binding    barrier    laboratory    elucidate    native    pathogens    structure    immune    immunity    assembled    assemblies    despite    form    molecule    conformational    oligomerization    innate    changing    dynamically    inhibited    dynamic    derive    regulated    membranes    invading    combat    ruptures    antimicrobial    causing    our    composition    unanswered    gbp    cryo    day    membrane    gbps    little    fundamental    applicable    pronged    em    prevents    structural    employ    cells    pathogen    reconstitution    mechanistic    cell    ex    biology    mechanisms    guanylate    host    effector    off    supramolecular    once    avert    seeking    lacking    reshape    mobilized    structurally    inside    function    structures    phagosomes    proteins    first    fights    encounter    integrative    rearrange    interactions    disease    fluorescence    eliminated    model    formidable    phagosome    central    questions    effectors    shelter    visualize    rupture    environments    lysis    autonomous    physically    provides    molecular    single    imaging    broadly   

Project "PHAGOSCOPY" data sheet

The following table provides information about the project.

Coordinator
TECHNISCHE UNIVERSITEIT DELFT 

Organization address
address: STEVINWEG 1
city: DELFT
postcode: 2628 CN
website: www.tudelft.nl

contact info
title: n.a.
name: n.a.
surname: n.a.
function: n.a.
email: n.a.
telephone: n.a.
fax: n.a.

 Coordinator Country Netherlands [NL]
 Total cost 1˙438˙510 €
 EC max contribution 1˙438˙510 € (100%)
 Programme 1. H2020-EU.1.1. (EXCELLENT SCIENCE - European Research Council (ERC))
 Code Call ERC-2019-STG
 Funding Scheme ERC-STG
 Starting year 2020
 Duration (year-month-day) from 2020-02-01   to  2025-01-31

 Partnership

Take a look of project's partnership.

# participants  country  role  EC contrib. [€] 
1    TECHNISCHE UNIVERSITEIT DELFT NL (DELFT) coordinator 1˙438˙510.00

Map

 Project objective

Our immune system provides a formidable barrier to the many microbial pathogens that we encounter every day. Yet, many pathogens have the ability to avert this barrier by invading the host cell and seeking shelter inside a phagosome whose membrane physically prevents the pathogen from being recognized and eliminated. Cell-autonomous immunity is a part of the innate immune system that fights off such pathogens. Among the antimicrobial effectors mobilized by this immune response are the Guanylate-Binding Proteins (GBPs). GBPs form dynamic supramolecular assemblies that promote lysis of phagosomes and, thus, killing of pathogens. Despite their central importance, we know very little about the molecular mechanisms of GBPs. Two fundamental questions are: (1) What is the structure and composition of GBP assemblies on membranes?, and (2) Once assembled, how do the GBPs structurally rearrange to reshape and rupture the phagosome's membrane? These questions remain unanswered because structural biology has been lacking methods for determining dynamically changing structures of proteins that are assembled in complex environments such as phagosomes. Here, I propose to take a two-pronged approach to address these questions: first, I will use cryo-EM and (single-molecule) fluorescence microscopy to elucidate the interactions and conformational changes involved in GBP oligomerization on model membranes. Second, I will visualize this pathway on native phagosomes using a recently developed ex vivo reconstitution system unique to my laboratory. By determining how GBP assemblies form on phagosome membranes, how they reshape the membrane so that it ruptures, and how these processes can be regulated and inhibited, I will derive a mechanistic model of a key effector function that cells employ to combat disease-causing pathogens. More broadly, my study will establish a novel approach for integrative imaging that will be applicable to a wide range of dynamic molecular assemblies in cells.

Are you the coordinator (or a participant) of this project? Plaese send me more information about the "PHAGOSCOPY" project.

For instance: the website url (it has not provided by EU-opendata yet), the logo, a more detailed description of the project (in plain text as a rtf file or a word file), some pictures (as picture files, not embedded into any word file), twitter account, linkedin page, etc.

Send me an  email (fabio@fabiodisconzi.com) and I put them in your project's page as son as possible.

Thanks. And then put a link of this page into your project's website.

The information about "PHAGOSCOPY" are provided by the European Opendata Portal: CORDIS opendata.

More projects from the same programme (H2020-EU.1.1.)

NanoPD_P (2020)

High throughput multiplexed trace-analyte screening for diagnostics applications

Read More  

sociOlfa (2020)

Learning from social scents: from territory to identity

Read More  

CN Identity (2019)

Comprehensive anatomical, genetic and functional identification of cerebellar nuclei neurons and their roles in sensorimotor tasks

Read More